CircRNA in ocular neovascular diseases: Fundamental mechanism and clinical potential

Zhang, Wenxin; He, Yuxi; Zhang, Yan

doi:10.1016/j.phrs.2023.106946

Cited by 10 publications

(1 citation statement)

References 181 publications

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“…Circular RNAs (circRNAs) are a special kind of noncoding RNA with a closed loop structure and extremely stable expression (10–12). The important regulatory effect of circRNA on cell biological functions in human disease has been confirmed (13–16). For example, it was found that circ-HIPK2 could promote myocardial oxidative injury by increasing cell apoptosis and autophagy (17).…”

Section: Introductionmentioning

confidence: 91%

Circvma21-Related Pathway Alleviates Lipopolysaccharide-Induced Hk-2 Cell Injury

Li,

Fu,

Zeng

et al. 2024

Shock

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Background It is reported that circVMA21 has an inhibition effect on sepsis-induced acute kidney injury (AKI). Therefore, the underlying molecular mechanisms of circVMA21 in AKI are worthy of further investigation. Material and methods Lipopolysaccharide (LPS) was used to induce HK2 cell injury. CircVMA21, miR-337-3p and ZEB2 expression was tested by qRT-PCR. Cell growth was detected by CCK8 assay, EdU assay and flow cytometry. Protein levels were examined by western blot. The levels of inflammatory factors and oxidative stress markers were measured to evaluate cell inflammatory response and oxidative stress. RNA relationship as verified by dual-luciferase reporter assay, RIP assay and RNA pull-down assay. Results CircVMA21 had decreased expression in AKI patients. Overexpressed circVMA21 alleviated LPS-induced HK2 cell inflammation, apoptosis, and oxidative stress. Moreover, circVMA21 sponged miR-337-3p, and miR-337-3p targeted ZEB2. The inhibitory effect of circVMA21 on LPS-induced HK2 cell injury was reversed by miR-337-3p overexpression, and ZEB2 overexpression abolished the promotion effect of miR-337-3p on LPS-induced HK2 cell injury. Conclusions CircVMA21 could inhibit LPS-induced HK2 cell injury via miR-337-3p/ZEB2 axis.

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Section: Introductionmentioning

confidence: 91%