CircRNA PLOD2 enhances ovarian cancer propagation by controlling miR-378

Abstract

It has been confirmed that circular RNA participates in tumorgenesis through a variety of ways, so it may be used as a molecular marker for tumor diagnosis and treatment. In this study, the expression of circ-LOPD2 in ovarian cancer tissues and cell lines was detected by qRT-PCR and Western blot. The dual luciferase report was used to verify the target of circ-LOPD2, and the silencing and overexpression of circ-CSPP1 in cell lines was used to explore its relationship with miRNA-378. The cell proliferation was detected by CCK8 method, and the expression level of miRNA-378 was detected by qRT-PCR. The results showed that circ-LOPD2 was highly expressed in ovarian cancer (OC) tissues, circ-LOPD2 expression levels were higher in OVCAR3 and A2780, and circ-LOPD2 expression levels in CAOV3 were lower. After silencing circ-LOPD2, the growth ability of OVCAR3 and A2780 cells decreased, while overexpression of circ-LOPD2 led to the opposite result. We also found that miR-378 is a target of circ-LOPD2. Silencing circ-LOPD2 will increase the expression of miR-378, and overexpression of circ-LOPD2 will decrease the expression of miR-378. In summary, our results show that circ-LOPD2 as a miR-378 sponge promotes the proliferation of ovarian cancer cells, which may in turn promote the development of OC.

Circular RNA circPLOD2 regulates pericyte function by targeting the transcription factor KLF4

“…We first looked at potential miRNA involvement. However, contrary to a previous report 17 , we observed neither a generally increased density of miRNA target sites nor an enrichment for any particular miRNA ( Figure S3B ). Moreover, in publicly available AGO2 pulldown data 18 , we only found two peaks of AGO2 binding in circPLOD2, presumably harboring two miRNAs, however, their predicted targets showed no directed regulation in circPLOD2-depleted pericytes ( Figure S3C ).…”

“…Moreover, in publicly available AGO2 pulldown data 18 , we only found two peaks of AGO2 binding in circPLOD2, presumably harboring two miRNAs, however, their predicted targets showed no directed regulation in circPLOD2-depleted pericytes ( Figure S3C ). Finally, target sites of the previously described circPLOD2-interacting miRNA miR-378 17 were neither predicted nor detected in the AGO2 pulldowns. Based on these observations, we concluded that circPLOD2 most likely does not interfere with miRNA regulation.…”

“…Genetic inactivation of KLF4 in perivascular cells furthermore decreased formation of a pre-metastatic niche and metastasis 32 . Given that the circRNA host gene PLOD2 is essential for the invasion and metastasis and that circPLOD2 was shown to augment proliferation of ovarian cancer cells 33 , one might speculate that silencing of circPLOD2 could reduce KLF4 expression to limit cancer progression. Together, these data show that further studies are necessary to elicit the role of KLF4 in perivascular cells, particularly in pericytes.…”

Depicting the Implication of miR-378a in Cancers

“…circ-LOPD2 expression is high in both OC tissue and cells and is responsible for promoting OC cell proliferation. 63 Several studies have revealed that circ-RNAs worked as molecule sponges of miR-378a to regulate cancer development.…”

Research progress of the netrins and their receptors in cancer

“…Ovarian cancer (OC) is the second leading cause of mortality among women with gynaecological cancers worldwide. 74 Previous research has discovered that NTN1 is overexpressed in the vast majority of malignant ovarian tumours but not in benign tumours. Moreover, the correlation between high NTN1 expression and tumour stage and grade suggests that NTN1 may perform an oncogene role in OC.…”