CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8

Abstract

Background/Aims: Circular RNAs (circRNAs) play a significant role in the development and progression of various human cancers. However, the expression and function of circRNAs in cervical cancer (CC) have rarely been explored. The aim of this study was to investigate the biological function of circRNA8924 in CC and elucidate the possible molecular mechanism involved. Methods: Quantitative polymerase chain reaction was used to determine mRNA expression of circRNA8924, miR-518d-5p/519-5p and CBX8 in CC tissues and cells. CBX8 protein expression was measured by Western blotting. The CCK-8 assay was used to evaluate cell proliferation, and the transwell assay to determine cell migration and invasion. The luciferase reporter assay was used to determine the direct regulation of miR-518d-5p/519-5p and circRNA8924 or CBX8 Results: The study demonstrated that the expression level of circRNA8924 in CC was significantly higher than that in the adjacent normal tissues (P < 0.001), and that it was also associated with tumor size, FIGO staging and myometrial invasion. The knockdown of circRNA8924 significantly inhibited the proliferation, migration and invasion of CC cells SiHa and HeLa. The expression level of miR-518d-5p/519-5p was negatively correlated with circRNA8924, and circRNA8924 regulated CBX8 by competitively binding to miR-518d-5p/519-5p. Conclusions: CircRNA8924 is highly expressed in CC tissue and can be considered a competitive endogenous RNA of the miR-518d-5p/519-5p family to promote the malignant biological behavior of CC cells. It is suggested that it may serve as a new biomarker for CC diagnosis and disease progression and provide potential targets for targeted therapy.

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

“…CSX8 is known as a member of CBX family and exerts oncogenic functions in some cancers. Liu et al reported that CBX8 could be regulated by miRNAs (miR-518d-5p/519-5p) at post-transcriptional level [25]. In hepatocellular carcinoma, CBX8 exhibits oncogenic properties by affecting AKT/β-catenin pathway [26].…”

Silibinin Induces G2/M Cell Cycle Arrest by Activating Drp1-Dependent Mitochondrial Fission in Cervical Cancer

“…Persistent infection with high-risk human papillomavirus (HPV) has been verified as the major risk factor in cervical cancer progression (Liu et al, 2018). More than 118 different types of HPV has been validated and the type of HPV 16 and 18 attributed to three quarters incidence rates (Crosbie et al, 2013).…”

<p>Hsa_circ_103973 Acts as a Sponge of miR-335 to Promote Cervical Cancer Progression</p>

“…For instance, circ_8924 was reported by Li W to promote the CC cell proliferation, migration and invasion by sponging miR-518e-5p family and regulating the expression of CBX8. 20 Moreover, results from Xianchao Kong el al showed that circ101996 might act as an oncogene of CC through increasing the expression of TPX2 through miR-8075. 21 In this study, a circRNA microarray analysis was conducted on two CC-associated circRNA GSE datasets (GSE102686 and GSE113696) downloaded from the GEO database (www.ncbi.nlm.nih.gov/geo) to screen specific circRNAs that might involve in the pathogenesis of CC.…”