circRNA_0000285 knockdown suppresses viability and promotes apoptosis of cervical cancer cells by sponging microRNA-654-3p

Zhang, Sisi; Xu, Yuping; Zheng, Qingyu

doi:10.1080/21655979.2022.2037870

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…These findings support hsa_circ_0000285's oncogenic role in HCC progression and are consistent with prior findings in other malignancies. 11,12,32,33 Accumulating research have confirmed that miR-582-3p exerts anti-tumor influence in HCC. [34][35][36] Elevated miR-582-3p levels in HCC cells has been documented to suppress migration, invasion, and proliferation.…”

Section: Discussionmentioning

confidence: 98%

“…Interestingly, previous research has suggested that the expression of hsa_circ_0000285 varies in different cancer types. For example, it was reported to be downregulated in bladder cancer tissues 10 , whereas it was upregulated in osteosarcoma cell lines, 11 tissues/cell lines, 12,32 and thyroid cancer cell lines. 33 Moreover, hsa_circ_0000285 was predominantly situated within the HCC cell cytoplasm and was not degraded by RNase R. Furthermore, hsa_circ_0000285 silencing inhibited tumor formation by repressing proliferation and migration of HCC cells and promoting apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…These findings support hsa_circ_0000285’s oncogenic role in HCC progression and are consistent with prior findings in other malignancies. 11,12,32,33…”

Section: Discussionmentioning

confidence: 99%

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Hsa_circ_0000285 knockdown inhibits the progression of hepatocellular carcinoma by sponging miR-582-3p to regulate CCNB2 expression

et al. 2022

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Aim Hsa_circ_0000285, a novel circular RNA, has been proven to extensively take part in the pathogenesis of numerous tumors. In hepatocellular carcinoma (HCC), very little is known about hsa_circ_0000285 until now. Hence, this research aims to determine hsa_circ_0000285’s functional role and underlying mechanisms in HCC. Methods The expressions of miR-582-3p, hsa_circ_000028, and cyclin B2 (CCNB2) among the HCC cells and tumor samples were determined by performing western blotting and qRT-PCR analyses. The impacts of hsa_circ_000028 on the proliferative and migratory abilities of HCC cells were examined through the execution of CCK-8 and wound-healing assays. Meanwhile, the expressions of the proteins Bcl-2 and Bax were detected via western blotting. Tumor xenograft models were established to examine how hsa_circ_000028 functions during the mediation of HCC tumor growth in vivo. RNA immunoprecipitation and luciferase reporter experiments were performed for the validation of the interactions of miR-582-3p, hsa_circ_000028, and CCNB2 with each other. Results Elevated hsa_circ_0000285 and CCNB2 expressions, and a decreased miR-582-3p expression were observed among the HCC cell lines and tumors. Hsa_circ_0000285 bound to miR-582-3p competitively to improve CCNB2 levels. Silencing of hsa_circ_0000285 promoted apoptosis and repressed proliferation and migration among HCC cells. Moreover, silencing hsa_circ_0000285 also impeded the growth of HCC tumors in vivo. Inhibiting hsa_circ_0000285 or CCNB2 reversed the miR-582-3p-knockdown-mediated promotion of malignant HCC cell phenotypes. Conclusion Our study has demonstrated that hsa_circ_0000285 fosters the development of malignant HCC cells phenotypes through the modulation of the miR-582-3p/CCNB2 axis. Thus, these results suggest that hsa_circ_0000285 is a prospective target for HCC treatment.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Hsa_circ_0000285 knockdown inhibits the progression of hepatocellular carcinoma by sponging miR-582-3p to regulate CCNB2 expression

et al. 2022

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“…GLRA2, the glycine receptor subunit, is a specific target of miR-654-3p [ 66 ]. Several research articles pointed to the involvement of miR-654-3p in tumorigenesis across various contexts [ 101 , 102 ]. However, in epilepsy research, there are only two studies regarding miR-654-3p [ 45 , 59 ].…”

Section: Candidate C-mirnas With Biomarker Potential In Tle Patientsmentioning

confidence: 99%

Circulating miRNAs as Novel Clinical Biomarkers in Temporal Lobe Epilepsy

Guarnieri,

Amodio,

Bosco

et al. 2024

ncRNA

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Temporal lobe epilepsy (TLE) represents the most common form of refractory focal epilepsy. The identification of innovative clinical biomarkers capable of categorizing patients with TLE, allowing for improved treatment and outcomes, still represents an unmet need. Circulating microRNAs (c-miRNAs) are short non-coding RNAs detectable in body fluids, which play crucial roles in the regulation of gene expression. Their characteristics, including extracellular stability, detectability through non-invasive methods, and responsiveness to pathological changes and/or therapeutic interventions, make them promising candidate biomarkers in various disease settings. Recent research has investigated c-miRNAs in various bodily fluids, including serum, plasma, and cerebrospinal fluid, of TLE patients. Despite some discrepancies in methodologies, cohort composition, and normalization strategies, a common dysregulated signature of c-miRNAs has emerged across different studies, providing the basis for using c-miRNAs as novel biomarkers for TLE patient management.

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“…SiRNA-induced knockdown of circ_0000285 increased miR-654-3p expression, diminishing cell viability, increasing cell apoptosis, and suppressing the CC progression. Thereby, circ_0000285 down-modulation can desirably be in line with CC deceleration [ 118 ].…”

Section: How Circular Rnas Control Cervical Cancer Progression? a Foc...mentioning

confidence: 99%

Circular RNAs and cervical cancer: friends or foes? A landscape on circRNA-mediated regulation of key signaling pathways involved in the onset and progression of HPV-related cervical neoplasms

Heydarnia,

Dorostgou,

Hedayati

et al. 2024

Cell Commun Signal

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Cervical cancer (CC) is a common gynecologic malignancy, accounting for a significant proportion of women death worldwide. Human papillomavirus (HPV) infection is one of the major etiological causes leading to CC onset; however, genetic, and epigenetic factors are also responsible for disease expansion. Circular RNAs (circRNAs), which are known as a particular subset of non-coding RNA (ncRNA) superfamily, with covalently closed loop structures, have been reported to be involved in the progression of diverse diseases, especially neoplasms. In this framework, abnormally expressed circRNAs are in strong correlation with CC pathogenesis through regulating substantial signaling pathways. Also, these RNA molecules can be considered as promising biomarkers and therapeutic targets for CC diagnosis/prognosis and treatment, respectively. Herein, we first review key molecular mechanisms, including Wnt/β-catenin, MAPK, and PI3K/Akt/mTOR signaling pathways, as well as angiogenesis and metastasis, by which circRNAs interfere with CC development. Then, diagnostic, prognostic, and therapeutic potentials of these ncRNA molecules will be highlighted in depth.

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circRNA_0000285 knockdown suppresses viability and promotes apoptosis of cervical cancer cells by sponging microRNA-654-3p

Cited by 8 publications

References 43 publications

Hsa_circ_0000285 knockdown inhibits the progression of hepatocellular carcinoma by sponging miR-582-3p to regulate CCNB2 expression

Hsa_circ_0000285 knockdown inhibits the progression of hepatocellular carcinoma by sponging miR-582-3p to regulate CCNB2 expression

Circulating miRNAs as Novel Clinical Biomarkers in Temporal Lobe Epilepsy

Circular RNAs and cervical cancer: friends or foes? A landscape on circRNA-mediated regulation of key signaling pathways involved in the onset and progression of HPV-related cervical neoplasms

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