circRNA_0001006 predicts prognosis and regulates cellular processes of triple-negative breast cancer via miR-424-5p

Abstract

Background circular RNAs (circRNAs) have been considered novel biomarker candidates for human cancers, such as triple-negative breast cancer (TNBC). circ_0001006 was identified as a differentially expressed circRNA in metastatic breast cancer, but its significance and function in TNBC were unclear. The significance of circ_0001006 in TNBC was assessed and exploring its potential molecular mechanism to provide a therapeutic target for TNBC. Results circ_0001006 showed significant upregulation in TNBC and close association with patients’ histological grade, Ki67 level, and TNM stage. Upregulated circ_0001006 could predict a worse prognosis and high risk of TNBC patients. In TNBC cells, silencing circ_0001006 suppressed cell proliferation, migration, and invasion. In mechanism, circ_0001006 could negatively regulate miR-424-5p, which mediated the inhibition of cellular processes by circ_0001006 knockdown. Conclusions Upregulated circ_0001006 in TNBC served as a poor prognosis predictor and tumor promoter via negatively regulating miR-424-5p.

Synergistic Immunoregulation: harnessing CircRNAs and PiRNAs to Amplify PD-1/PD-L1 Inhibition Therapy

“…51 Briefly, circRNAs form a closed loop structure, devoid of the typical 5' cap or 3' poly-A tail found in linear RNAs. 52 This circularity arises through a process called back-splicing, wherein a downstream splice site joins with an upstream splice site within the same RNA molecule. 52 Their closed-loop configuration renders them less susceptible to degradation by exonucleases, enzymes that typically target linear RNAs by accessing their exposed ends.…”

“…52 This circularity arises through a process called back-splicing, wherein a downstream splice site joins with an upstream splice site within the same RNA molecule. 52 Their closed-loop configuration renders them less susceptible to degradation by exonucleases, enzymes that typically target linear RNAs by accessing their exposed ends. Thus, circRNAs possess higher stability compared to their linear counterparts.…”

“…Thus, circRNAs possess higher stability compared to their linear counterparts. [50][51][52] Recent evidence has gradually uncovered the multifaceted roles of circRNAs, such as regulating gene expression through interactions with microRNAs (miRNAs) and proteins, sequestrating RNAbinding proteins, and serving as templates for translation. 17,53 Moreover, the circular architecture of circRNAs underpins their unique stability and functionality.…”