CircRNA_000864 Upregulates B-cell Translocation Gene 2 Expression and Represses Migration and Invasion in Pancreatic Cancer Cells by Binding to miR-361-3p

Abstract

BackgroundPancreatic cancer is a fatal disease with a very poor prognosis due to its characteristic insidious symptoms, early metastasis, and chemoresistance. Circular RNAs (circRNAs) are involved in the development of pancreatic cancer.AimHence, the aim of this study is to elucidate the mechanism of circRNA_000864 in regulating BTG2 expression in pancreatic cancer by binding to miR-361-3p.MethodsCircRNA_000864, miR-361-3p, and BTG2 expression patterns in the pancreatic cancer tissues were detected by RT-qPCR. Correlation among circRNA_000864, miR-361-3p, and BTG2 was evaluated by RNA-pull down assay, RNA Immunoprecipitation assay, and dual-luciferase reporter gene assay. After ectopic expression and depletion experiments, 5-ethynyl-2′-deoxyuridine assay, Transwell assay, and flow cytometry were employed to assess the cell proliferation, migration and invasion, cell cycle, and apoptosis. Xenotransplantation of nude mice was conducted to detect the effects of circRNA_000864, miR-361-3p, and BTG2 on tumor growth.ResultsCircRNA_000864 and BTG2 were poorly expressed, and miR-361-3p was highly expressed in the pancreatic cancer tissues. CircRNA_000864 bound to miR-361-3p could target BTG2. Cell proliferation, migration, and invasion were inhibited, and the cell cycle arrest and apoptosis were stimulated after overexpression of circRNA_000864 or BTG2 or downregulation of miR-361-3p. Overexpression of circRNA_000864 or downregulation of miR-361-3p also decreased the tumor growth in vivo.ConclusionsConjointly, our findings elicited that the overexpression of circRNA_000864 could promote BTG2 expression to inhibit pancreatic cancer development by binding to miR-361-3p, which represents an appealing therapeutic target for the treatment of pancreatic cancer.

CircRNF13 Promotes the Malignant Progression of Pancreatic Cancer through Targeting miR-139-5p/IGF1R Axis

“…Previous studies have identified mounting types of circRNAs playing irreplaceable roles in carcinogenesis [23][24][25]. demonstrated that circRNA_000864 upregulated B-cell translocation gene 2 expression and repressed migration and invasion in PC cells by binding to miR-361-3p [30].…”

Circular RNA CircPVT1 Inhibits 5-Fluorouracil Chemosensitivity by Regulating Ferroptosis Through MiR-30a-5p/FZD3 Axis in Esophageal Cancer Cells

“…Jian et al found that circ_001680 affected the proliferation and migration of colorectal cancer and mediated its chemoresistance by regulating BMI1 through miR-340 (26). In addition, some other reports also found circRNAs modulated chemoresistance in pancreatic cancer, lung cancer, and osteosarcoma (27)(28)(29). However, only a few studies investigated the role of circRNAs in regulating chemoresistance in ESCC.…”

CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway

“…In cervical cancer, circNEIL3 promotes tumorigenesis through the regulation of KLF12 by acting as a ceRNA of miR-137 [ 5 ]. Additionally, studies have demonstrated that cyclic circRNA_000864 absorbs miR-361, relieves the inhibitory effects on the target gene BTG2 in the pancreatic cancer, and promoted tumor proliferation and tumorigenesis [ 6 ]. Moreover, circLARP4 inhibited the development and metastasis of gastric cancer cells by targeting the miR-424/LATS1 axis [ 8 ].…”