CircRNAs in lung cancer - Biogenesis, function and clinical implication

Wang, Chengdi; Tan, Shuangyan; Li, Jingwei; Liu, Wenrong; Peng, Yong; Liu, Weimin

doi:10.1016/j.canlet.2020.08.013

Cited by 109 publications

(81 citation statements)

References 94 publications

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“…However, functional characterization of circRNAs is required prior to clinical applications. 13 Recent studies have reported different roles of circ-MYBL2 in different cancers. However, the involvement of circ-MYBL2 in NSCLC is unknown.…”

Section: Introductionmentioning

confidence: 99%

A Cytoplasm-Enriched circRNA circ-MYBL2 is Downregulated in Non-Small Cell Lung Cancer and Sponges Oncogenic miR-28 to Regulate Cancer Cell Proliferation and Apoptosis

Mao

Wang

2021

CMAR

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Background Recent studies have reported different roles of circRNA circ-MYBL2 in different cancers. However, the involvement of circ-MYBL2 in non-small cell lung cancer (NSCLC) is unknown. This study was carried out to explore the role of circ-MYBL2 in NSCLC. Methods The expression of circ-MYBL2 and miR-28 was detected by RT-qPCR. A 5-year follow-up study was performed for survival analysis. Nuclear fractionation assay was used for subcellular localization analysis. RNA pull-down assay was performed to detect the interaction between circ-MYBL2 and miR-28. The role of circ-MYBL2 and miR-28 in regulating the expression of each other was evaluated by overexpression assay. BrdU incorporation assay and cell apoptosis assay were performed to investigate the role of circ-MYBL2 and miR-28 in cell proliferation and apoptosis. Results NSCLC tissues exhibited significantly higher expression levels of miR-28 and lower expression levels of circ-MYBL2. Close correlations between circ-MYBL2 and miR-28 and patients’ survival were observed. Circ-MYBL2, which was found to be mainly enriched in cytoplasm, directly interacted with miR-28. Although circ-MYBL2 and miR-28 showed no regulatory role in the expression of each other, circ-MYBL2 suppressed the effects of miR-28 on cell proliferation and apoptosis. Conclusion Circ-MYBL2 is enriched in cytoplasm, and it sponges oncogenic miR-28 to suppress cancer cell proliferation in NSCLC and promote cell apoptosis.

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Section: Introductionmentioning

confidence: 99%

A Cytoplasm-Enriched circRNA circ-MYBL2 is Downregulated in Non-Small Cell Lung Cancer and Sponges Oncogenic miR-28 to Regulate Cancer Cell Proliferation and Apoptosis

Mao

Wang

2021

CMAR

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“…The role of circRNA in lung cancer has been widely studied, but as a biomarker its mechanism of action still need to be further clarified ( 34 , 35 ). Zhu et al ( 36 ) first used microarray chip technology to screen circRNA in lung adenocarcinoma tissue and found that the expression of nearly 60 circRNA molecules is changed.…”

Section: Discussionmentioning

confidence: 99%

circCD151 promotes GLI2 expression by regulating miR‑30d‑5p and enhancing proliferation, invasion and stemness of lung cancer

2021

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To investigate the changes of circular (circ)rna circcd151 expression in lung cancer tissues and cells and its effects on proliferation, migration and invasion of lung cancer cells. The relative expression levels of circcd151 in lung cancer tissues and lung cancer cells (a549 and nci-H292) were determined by reverse transcription-quantitative Pcr. The effects of silencing or upregulation of circcd151 on the activity and clonal forming ability of a549 and nci-H292 cells were detected by ccK-8 and cloning formation experiments. Transwell invasion assay detected the effects of silencing or upregulation of circcd151 on the migration and invasion ability of a549 and nci-H292 cells. The regulatory effect of circcd151 on mir-30d-5p was detected by dual luciferase reporter gene. The relative expression level of circcd151 in lung cancer tissues was significantly higher compared with that in adjacent tissues. The relative expression level of circcd151 in A549 and NCI-H292 cells was significantly higher compared with that in human lung epithelial cells. in a549 and nci-H292 cells, silencing circcd151 decreased cell activity and clonal formation ability and invasion ability was also significantly decreased. circcd151 was upregulated in a549 and nci-H292 cells and the activity and clonal formation ability of a549 and NCI-H292 cells were significantly increased and the invasion ability was also significantly increased. double luciferase reporter assay confirmed the ceRNA regulatory mechanism of circcd151/mir-30d-5p/Gli2. in the present study, in vivo and in vitro functional studies demonstrated that circcd151 may promote the proliferation, invasion and cell stemness of lung cancer cells. Further molecular mechanism studies demonstrated that circcd151 could promote the malignant proliferation of lung adenocarcinoma by targeting mir-30d-5p and upregulating Gli2 expression. From the perspective of circrna, the present study will provide new clues to the pathogenesis and prognostic judgment of lung adenocarcinoma and provide a new target for clinical treatment.

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Section: Introductionmentioning

confidence: 99%

“…Lung cancer is one of the most prevalent malignant neoplasms with highest mortality (1). According to its histological classification, lung cancer consists of smallcell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) (1). SCLC, accounting for around 13-15% of lung cancer, contributes to an extremely poor prognosis for patients, with approximate 5-year overall survival (OS) of 5% for its extensive stage (2).…”

Section: Introductionmentioning

confidence: 99%

Artificial intelligence-assisted decision making for prognosis and drug efficacy prediction in lung cancer patients: a narrative review

Li¹,

Wu²,

Zhao³

et al. 2021

J Thorac Dis

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In this review, we aim to present frontier studies in patients with lung cancer as it related to artificial intelligence (AI)-assisted decision-making and summarize the latest advances, challenges and future trend in this field.Background: Despite increasing survival rate in cancer patients over the last decades, lung cancer remains one of the leading causes of death worldwide. The early diagnosis, accurate evaluation and individualized treatment are vital approaches to improve the survival rate of patients with lung cancer. Thus, decision making based on these approaches requires accuracy and efficiency beyond manpower. Recent advances in AI and precision medicine have provided a fertile environment for the development of AI-based models. These models have the potential to assist radiologists and oncologists in detecting lung cancer, predicting prognosis and developing personalized treatment plans for better outcomes of the patients.Methods: We searched literature from 2000 through July 31 th , 2021 in Medline/PubMed, the Web of Science, the Cochrane Library, ACM Digital Library, INSPEC and EMBASE. Key words such as "artificial intelligence", "AI", "deep learning", "lung cancer", "NSCLC", "SCLC" were combined to identify related literatures. These literatures were then selected by two independent authors. Articles chosen by only one author will be examined by another author to determine whether this article was relative and valuable. The selected literatures were read by all authors and discussed to draw reliable conclusions. Conclusions: AI, especially for those based on deep learning and radiomics, is capable of assisting clinical decision making from many aspects, for its quantitatively interpretation of patients' information and its potential to deal with the dynamics, individual differences and heterogeneity of lung cancer. Hopefully, remaining problems such as insufficient data and poor interpretability may be solved to put AI-based models into clinical practice.

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CircRNAs in lung cancer - Biogenesis, function and clinical implication

Cited by 109 publications

References 94 publications

A Cytoplasm-Enriched circRNA circ-MYBL2 is Downregulated in Non-Small Cell Lung Cancer and Sponges Oncogenic miR-28 to Regulate Cancer Cell Proliferation and Apoptosis

A Cytoplasm-Enriched circRNA circ-MYBL2 is Downregulated in Non-Small Cell Lung Cancer and Sponges Oncogenic miR-28 to Regulate Cancer Cell Proliferation and Apoptosis

circCD151 promotes GLI2 expression by regulating miR‑30d‑5p and enhancing proliferation, invasion and stemness of lung cancer

Artificial intelligence-assisted decision making for prognosis and drug efficacy prediction in lung cancer patients: a narrative review

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