CircTMCO3 Promotes Gastric Cancer Progression by Regulating miR-577/RAB14 Axis

Abstract

Background Growing evidence indicated that circRNAs played major roles in the progression of human cancer. Nevertheless, the molecular mechanism and effects of circTMCO3 in GC are still unclear. Methods First, qRT-PCR was used to evaluate the levels of circTMCO3 from GC tissues, GC cells, normal tissues and gastric epithelial cells. Then, the GC cells were transfected to analyze the proliferation, migration and invasion of GC cells by MTT, colony formation and transwell assays. Next, the expressions of miR-577 and RAB14 in GC tissues and cells were examined by qRT-PCR following transfection. The target interaction of circTMCO3-miR-577 and miR-577-RAB14 was explored by the dual-luciferase reporter and RNA pull-down assays. In the end, the growth and viability of GC cells were detected by MTT, colony formation and transwell assays, respectively, following the transfection of GC cells. Results In this research, we found circTMCO3 expressions are significantly up-regulated in GC tissues and cells compared with the normal tissues and gastric epithelial cells. We discovered that the knockdown of circTMCO3 remarkably inhibits the proliferation, migration and invasion of GC cells. Besides, through the prediction of binding sites between circTMCO3, miR-577 and RAB14, we discovered miR-577 is a target of circTMCO3 while RAB14 is a target gene of miR-577. Finally, the results demonstrate the overexpression of miR-577 and the silence of RAB14 could inhibit the effects of circTMCO3 on proliferation, migration and invasion in GC cells. Conclusion circTMCO3 accelerated the growth and migration of GC cells by regulating miR-577/RAB14 axis.

Bioinformatics Analysis of the Mechanisms of Diabetic Nephropathy via Novel Biomarkers and Competing Endogenous RNA Network

“…Moreover, miR-374a-3p may regulate acute kidney injury by targeting TLR4/NF-κB pathway to reduce the rate of apoptosis and the generation of inflammatory cytokines ( 38 ). Most studies suggested that miR-577 and miR-944 are more commonly expressed in cancers, such as ovarian cancer ( 39 ), gastric cancer ( 40 ), and colorectal cancer ( 41 ), and little is known regarding in T2DM. Nevertheless, recent studies have shown that these genes also participate in fasting glucose control in diabetes via activating Akt signaling pathways and increasing insulin secretion ( 42 , 43 ).…”

Chronic stress promotes NSCLC progression through circMBOAT2 upregulation mediated by CTCF

“…circRNAs have been proven to play important roles in the occurrence and development of many diseases, such as cancer and depression(8-10). circRNAs have been found to be present in the blood, saliva, urine and cerebrospinal uid of tumor patients (11,12), and dysregulated circRNAs can act as oncogenes or tumor suppressors and may contribute to the development and progression of various cancers (13)(14)(15). Thus, can be used as a molecular marker for cancer diagnosis and treatment.…”

Circ_0058058 Drives the Malignant Phenotypes and Immune Evasion of Pancreatic Cancer by the MicroRNA-557–Dependent Regulation of PDL1

“…For instance, Bai et al 19 showed that circ_0007334 contributed to tumor cell growth and angiogenesis by mediating the microRNA-557-5p (miR-557)/KLF12 axis in colorectal cancer. CircTMCO3 accelerated the growth and migration of gastric cancer cells by regulating miR-557/RAB14 axis 20 . In PC, it has been revealed that miR-557 represses PC process via targeting the SLC7A11/PI3K/AKT pathway axis 21 .…”

“…CircTMCO3 accelerated the growth and migration of gastric cancer cells by regulating miR-557/RAB14 axis. 20 In PC, it has been revealed that miR-557 represses PC process via targeting the SLC7A11/PI3K/AKT pathway axis. 21 However, mechanisms mediated by miR-557 in PC are complex and need further investigation.…”