CircTUBGCP3 facilitates the tumorigenesis of lung adenocarcinoma by sponging miR-885-3p

Abstract

Background Circular RNAs (circRNAs) act pivotal roles in the progression of multiple malignancies. However, the underlying mechanisms by which hsa_circ_0007031 (circTUBGCP3) contributes to lung adenocarcinoma (LAC) remain largely unknown. Methods The association of circTUBGCP3 expression with clinicopathological characteristics and prognosis in patients with LAC was determined by RT-qPCR and fluorescence in situ hybridization. The in vitro functional experiments as well as a subcutaneous tumorigenesis model were executed to estimate the role of circTUBGCP3 in LAC cells. The interaction between circTUBGCP3 and miR-885-3p was confirmed by RNA immunoprecipitation, luciferase gene report and RT-qPCR assays. The effects of circTUBGCP3 on miR-885-3p-mediated Wnt10b/β-catenin signaling were evaluated by Western blot. Results The upregulation of circTUBGCP3 or downregulation of miR-885-3p was associated with the pathological stage and poor survival in patients with LAC. Restored expression of circTUBGCP3 facilitated the growth and invasion of LAC cells, but knockdown of circTUBGCP3 harbored the opposite effects. In mechanism, circTUBGCP3 could act as a sponge of miR-885-3p, which suppressed the cell proliferation and colony formation and attenuated the tumor-promoting effects of circTUBGCP3. Wnt10b as a target of miR-885-3p could be upregulated be circTUBGCP3 and indicate poor survival in patient with LAC. Conclusions Our findings demonstrated that circTUBGCP3 promoted LAC progression by sponging miR-885-3p, and might represent a prognostic factor for LAC.

The role of WNT10B in physiology and disease: A 10-year update

“…Knocking down circTUBGPC3 inhibited WNT10B expression while inhibiting miR-885-3p upregulated WNT10B in NCI-H460 cells. Overall, this study concluded that circTUBGPC3 promotes lung cancer progression by inhibiting miR-885-3p, thus allowing for the overexpression of WNT10B ( Yang Y. et al, 2021 ). Only one paper has investigated WNT10B in the progression of lung cancer, and further work should be carried out to elucidate the actions of WNT10B in normal lung cells, lung fibrosis, and lung cancer.…”

“…Furthermore, Yang et al (2021) reported that WNT10B was regulated by non-coding RNAs. Their study identified an overexpression of the circular RNA circTUBGPC3 in lung adenocarcinoma (LAC) patients ( Table 1 ), and high expression of circTUBGPC3 correlated with worse overall survival.…”

Role of circular RNAs in lung cancer

“…Another circRNA associated with LUAD is circTUBGCP3. Upregulation of this circRNA, or downregulation of miR-885-3p, was associated with pathological stage and poor survival in LUAD patients ( Yang et al, 2021 ). Mechanistically, sponging of miR-885-3p by circTUBGCP3 prevents the miRNA from suppressing cell proliferation and attenuated the tumor-promoting effects of circTUBGGCP3.…”

Non-Coding RNAs as Key Regulators in Lung Cancer

“…The vast majority of prognostic marker investigations in LC are based on the analysis of circRNA expression in tissues. A significant number of studies have been conducted on LUAD [ 195 , 196 , 197 , 198 , 199 ].…”

“…circTUBGCP3 can act as a “sponge” for miR-885-3p, the role of which is to suppress cell proliferation and weaken the tumour-promoting effect of circTUBGCP3. Wnt10b, a target of miR-885-3p, may be upregulated by circTUBGCP3 and indicate poor survival in LC patients and points to the poor survival of patients with LC [ 198 ].…”