circTUBGCP5 promotes liver cancer progression and glycolysis by up-regulating the expression of ACSL4

Abstract

A large number of researches have shown that circular RNA (circRNA) is new hope for the diagnosis or treatment of tumors, including liver cancer (LCa). However, it remains largely unclear the role of circRNA in the progression of LCa and its molecular mechanism. GSE164803 microarray dataset was applied to identify dysregulated circRNAs in LCa and noncancerous tissues. CircTUBGCP5 (hsa_circ_0034049) was selected for further research. Biological functions of circTUBGCP5 were investigated by EdU, colony formation, flow cytometry, glucose consumption and lactate production assay, and in vivo tumorigenesis. RNA pull-down assay and dual-luciferase reporter assay were used to investigate the interaction between circTUBGCP5, miR-144-3p, and ACSL4. We demonstrated that circTUBGCP5 was significantly up-regulated in LCa tissues and cells. CircTUBGCP5 promoted LCa cell proliferation, anti-apoptotic ability, glycolysis, and tumorigenesis at least partially by sponging miR-144-3p to regulate ACSL4 protein level. In conclusion, circTUBGCP5 is a forceful contributor to malignant behaviors and glycolysis of LCa via modulating the circTUBGCP5/miR-144-3p/ACSL4 axis, which has provided a target for the diagnosis and treatment of LCa patients.

Exploring the impact of circRNAs on cancer glycolysis: Insights into tumor progression and therapeutic strategies

“… [ 110 ] HCC circ_0000517 Promotes Glycolysis By regulating miR-326 and IGF1R, circ_0000517 knockdown inhibited the development of HCC in both in vitro and in vivo. [ 75 ] Liver cancer circTUBGCP5 Promotes Glycolysis circ-TUBGCP5 significantly influences the aggressive characteristics and glycolysis of liver cancer by regulating the circThe TUBGCP5/miR-144-3p/ACSL4 axis [ 331 ] GC circRPS19 Promotes glycolysis Through the miR-125a-5p/USP7 axis, circRPS19 stabilized the HK2 protein, causing aerobic glycolysis in GC cells and accelerating the growth of GC. [ 155 ] GC circ_0067514 Inhibits glycolysis circ_0067514 inhibited the progression of GC and glycolysis by affecting the miR-654-3p/LATS2 pathway.…”