Circular RNA circ-LDLRAD3 serves as an oncogene to promote non-small cell lung cancer progression by upregulating SLC1A5 through sponging miR-137

Xue, Min; Hong, Weijun; Jiang, Jun; Zhao, Fang; Gao, Xiwen

doi:10.1080/15476286.2020.1789819

Cited by 35 publications

(32 citation statements)

References 38 publications

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“…The human fetal osteoblastic cell line (hFOB1. 19), human osteosarcoma cell lines (KHOS and U2OS), and 293T cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Under the humidified air atmosphere containing 5% CO 2 at 37°C, cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Several reports have exhibited that the abnormal expression of miRNAs is associated with drug resistance in many types of cancer, including osteosarcoma [ 16 – 18 ]. MicroRNA-137 (miR-137) displayed abnormally low expression in human cancers, such as lung cancer [ 19 ], hepatocellular carcinoma [ 20 ], and osteosarcoma [ 21 ]. Importantly, the relevant literature reported that miR-137 was decreased in DXR-resistant osteosarcoma cell lines, and the overexpression of miR-137 could weaken the DXR resistance of osteosarcoma cells [ 22 ], suggesting the pivotal role of miR-137 in DXR resistance in osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137

Huang

Wang

2021

BioMed Research International

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Background. Chemoresistance is a major obstacle to the treatment of osteosarcoma patients. Circular RNA (circRNA) circPVT1 has been reported to be related to the doxorubicin (DXR) resistance in osteosarcoma. This study is designed to explore the role and mechanism of circPVT1 in the DXR resistance of osteosarcoma. Methods. circPVT1, microRNA-137 (miR-137), and TP53-regulated inhibitor of apoptosis 1 (TRIAP1) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The protein levels of ATP-binding cassette, subfamily C, member 1 (ABCC1), multidrug resistance-associated protein 1 (MRP-1), cleaved- (c-) caspase-3, B-cell lymphoma-2 (Bcl-2), and TRIAP1 were examined by a western blot assay. Cell viability, proliferation, and apoptosis were detected by cell counting kit-8 (CCK-8), colony formation, and flow cytometry assays, severally. The binding relationship between miR-137 and circPVT1 or TRIAP1 was predicted by starbase 3.0 and then verified by dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. The biological role of circPVT1 in osteosarcoma tumor growth and drug resistance was examined by the xenograft tumor model in vivo. Results. circPVT1 and TRIAP1 were highly expressed, and miR-137 was decreased in DXR-resistant osteosarcoma tissues and cells. Moreover, circPVT1 knockdown could boost DXR sensitivity by inhibiting DXR-caused proliferation and DXR-induced apoptosis in DXR-resistant osteosarcoma cells in vitro. The mechanical analysis discovered that circPVT1 acted as a sponge of miR-137 to regulate TRIAP1 expression. circPVT1 silencing increased the drug sensitivity of osteosarcoma in vivo. Conclusion. circPVT1 boosted DXR resistance of osteosarcoma cells partly by regulating the miR-137/TRIAP1 axis, hinting a promising therapeutic target for the osteosarcoma treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137

Huang

Wang

2021

BioMed Research International

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“…Several circRNAs are upregulated in NSCLC, with oncogenic roles to positively regulate in vitro EMT processes. For instance, circ-LDLRAD3 promoted proliferation and EMT in NSCLC cells by downregulating miR-137, and subsequently upregulating glutamine transporter solute carrier family A1 member 5 (SLC1A5) ( 87 ). Notably, SLC1A5 was identified as participating in NSCLC progression and regulation, and similarly, its inactivation inhibited NSCLC cell viability ( 88 ).…”

Section: The Role Of Circrnas In Lung Cancermentioning

confidence: 99%

Circular RNAs in Lung Cancer: Recent Advances and Future Perspectives

Chen

Zhang

et al. 2021

Front. Oncol.

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Globally, lung cancer is the most commonly diagnosed cancer and carries with it the greatest mortality rate, with 5-year survival rates varying from 4–17% depending on stage and geographical differences. For decades, researchers have studied disease mechanisms, occurrence rates and disease development, however, the mechanisms underlying disease progression are not yet fully elucidated, thus an increased understanding of disease pathogenesis is key to developing new strategies towards specific disease diagnoses and targeted treatments. Circular RNAs (circRNAs) are a class of non-coding RNA widely expressed in eukaryotic cells, and participate in various biological processes implicated in human disease. Recent studies have indicated that circRNAs both positively and negatively regulate lung cancer cell proliferation, migration, invasion and apoptosis. Additionally, circRNAs could be promising biomarkers and targets for lung cancer therapies. This review systematically highlights recent advances in circRNA regulatory roles in lung cancer, and sheds light on their use as potential biomarkers and treatment targets for this disease.

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“…With respect to epigenetic regulation, a circular RNA, circ-LDLRAD3, has been found to upregulate SLC1A5 expression to promote lung cancer cell progression (37). Further, the epigenetic silencing of microRNA-137 in lung cancer enhances SLC1A5 expression to promote cancer cell growth (38).…”

Section: Construction Of the Ubiquitin-proteasome System (Ups) Regulatory Network Based On The Ubiquitinated Ferroptosis-related Proteinsmentioning

confidence: 99%

Identification of ubiquitinated substrate proteins and their gene expression patterns in lung adenocarcinoma

Xu¹,

Lu²,

Zhao³

et al. 2021

Ann Transl Med

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Background: Lung cancer is a malignant disease with the highest cancer-related mortality rate. In lung adenocarcinoma (LUAD), protein ubiquitination can regulate multiple biological processes. A LUAD ubiquitylome analysis has not yet been reported. Methods:We used for the first time ion mobility into liquid chromatography-mass spectrometry to perform accurate and reliable ubiquitylome and proteomic analysis of clinical LUAD and normal tissues and combined it with transcriptome data obtained from public databases. Ubiquitinated protein substrates and their gene expression pattern landscapes in LUAD were identified using bioinformatics methods.Results: Our data revealed a ubiquitination landscape in LUAD and identified characteristic protein ubiquitination motifs. We found that the ubiquitinated peptide motifs in LUAD were completely different from those of previously published lung squamous cell carcinoma (LUSC). Moreover, we identified two gene expression patterns of ubiquitinated proteins and revealed that survival differences between these patterns may be correlated with the tumor immune infiltrating microenvironment. Finally, we constructed a prognostic predictive model to quantify the relationship between expression patterns and survival. We found a relationship between the patient-applied model score and multiple drug sensitivity. Therefore, our model can serve as a guide for LUAD clinical treatment.Conclusions: Our work addresses the lack of ubiquitylome studies in LUAD and provides new perspectives for subsequent research and clinical treatment.

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Circular RNA circ-LDLRAD3 serves as an oncogene to promote non-small cell lung cancer progression by upregulating SLC1A5 through sponging miR-137

Cited by 35 publications

References 38 publications

Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137

Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137

Circular RNAs in Lung Cancer: Recent Advances and Future Perspectives

Identification of ubiquitinated substrate proteins and their gene expression patterns in lung adenocarcinoma

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