Circular RNA circ_0004277 Inhibits Acute Myeloid Leukemia Progression Through MicroRNA-134-5p / Single stranded DNA binding protein 2

Liu, Yao; Chen, Xi; Liu, Jingyang; Jin, Yinglan; Wang, Wei

doi:10.1080/21655979.2022.2059609

Cited by 18 publications

(12 citation statements)

References 32 publications

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“…A previous study showed that high expression of miR-134-5p promotes the relapse of early-stage lung adenocarcinoma, leading to poor survival 26 . Other studies have shown that miR-134-5p suppresses the aggressiveness and growth of AML cells 27 . However, the role of miR-134-5p in HCC remains elusive.…”

Section: Discussionmentioning

confidence: 93%

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Zhou

Chen

et al. 2022

Preprint

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Background Hepatocellular carcinoma (HCC) ranks the sixth most common cancer in the number of malignancies worldwide. Accumulating evidence indicated that circular RNAs (circRNAs) play vital roles in the progression of several malignancies. Nonetheless, the molecular mechanism and role of hsa_circ_0006421 in HCC are yet unclear. The present study aimed to verify the molecular mechanism and effects of hsa_circ_0006421 in HCC. Methods A total of 34 paired HCC tissues and paraneoplastic tissues surgically resected from patients were collected, and the expression of hsa_circ_0006421 in HCC tissues and the correlation with clinicopathological characteristics were analyzed. Then, CCK-8, colony formation, cell migration assay, transwell invasion assay, and Annexin-V/PI staining were used to assess the effects of hsa_circ_0006421 on the growth, migration, invasion, and apoptosis of HCC cells. Next, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect the expression of miR-134-5p, CELF2, and hsa_circ_0006421. Finally, the targeting interactions of miR-134-5p and hsa_circ_0006421, CELF2 and miR-134-5p were explored using the dual-luciferase reporter assay. Results Hsa_circ_0006421 was diminished in HCC tissues, and its downregulation was related to cirrhosis history. Hsa_circ_0006421 knockdown promoted HCC cell growth, migration, and invasion and inhibited apoptosis, whereas overexpression had opposite effects. Moreover, hsa_circ_0006421 served as the competing endogenous RNA of miR-134-5p. Subsequently, a reciprocal correlation between CELF2 and miR-134-5p was established. Hsa_circ_0006421 positively regulated the protein level of CELF2 in HCC. Conclusion Hsa_circ_0006421 inhibits liver cancer by regulating miR-134-5p/CELF2 axis.

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Section: Discussionmentioning

confidence: 93%

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Zhou

Chen

et al. 2022

Preprint

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“…CircPLXNB2 is a valuable predictor of prognosis in patients with AML (Lin, Wang, Bian, Sun, Guo, Kong, Zhao, Guo, Li, Wu, Wang, Wang and Li, 2021). Further, Liu et al (2022) found that expression of hsa_circ_0004277 can be restored after chemotherapy in patients with AML, suggesting that its up-regulation is associated with successful treatment; hence, hsa_circ_0004277 is a potential diagnostic marker and treatment target in AML. Further, circRNF220 can distinguish AML from ALL and other hematological malignancies, while high circRNF220 expression is an unfavorable prognostic marker of recurrence, due to its role in sequestration of miR-30a, which increases MYSM1 and IER2 expression and is implicated in AML relapse (Liu et al, 2021).…”

Section: Discussionmentioning

confidence: 98%

Predicting the influence of Circ_0059706 expression on prognosis in patients with acute myeloid leukemia using classical statistics and machine learning

Wen

et al. 2022

Front. Genet.

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Background: Various circular RNA (circRNA) molecules are abnormally expressed in acute myeloid leukemia (AML), and associated with disease occurrence and development, as well as patient prognosis. The roles of circ_0059706, a circRNA derived from ID1, in AML remain largely unclear.Results: Here, we reported circ_0059706 expression in de novo AML and its association with prognosis. We found that circ_0059706 expression was significantly lower in AML patients than in controls (p < 0.001). Survival analysis of patients with AML divided into two groups according to high and low circ_0059706 expression showed that overall survival (OS) of patients with high circ_0059706 expression was significantly longer than that of those with low expression (p < 0.05). Further, female patients with AML and those aged >60 years old in the high circ_0059706 expression group had longer OS than male patients and those younger than 60 years. Multiple regression analysis showed that circ_0059706 was an independent factor-affecting prognosis of all patients with AML. To evaluate the prospects for application of circ_0059706 in machine learning predictions, we developed seven types of algorithm. The gradient boosting (GB) model exhibited higher performance in prediction of 1-year prognosis and 3-year prognosis, with AUROC 0.796 and 0.847. We analyzed the importance of variables and found that circ_0059706 expression level was the first important variables among all 26 factors included in the GB algorithm, suggesting the importance of circ_0059706 in prediction model. Further, overexpression of circ_0059706 inhibited cell growth and increased apoptosis of leukemia cells in vitro.Conclusion: These results provide evidence that high expression of circ_0059706 is propitious for patient prognosis and suggest circ_0059706 as a potential new biomarker for diagnosis and prognosis evaluation in AML, with high predictive value and good prospects for application in machine learning algorithms.

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“…A previous study showed that high expression of miR-134-5p promotes the relapse of early-stage lung adenocarcinoma, leading to poor survival [26]. Other studies also indicated that miR-134-5p suppresses the aggressiveness and growth of AML cells [27]. However, the role of miR-134-5p in HCC remains elusive.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Deng

Zhou

Chen

et al. 2022

Preprint

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Background: Hepatocellular carcinoma (HCC) has ranked sixth most common cancer in number of malignancies in the worldwide. There is plenty of evidence indicated that circular RNAs (circRNAs) exert vitally important roles in the progression of many malignancies. Nevertheless, the molecular mechanism and role of hsa_circ_0006421 in HCC are still unclear. The aim of our research is to verify the molecular mechanism and effects of hsa_circ_0006421 in HCC.Methods: First, we collected 34 paired HCC tissues and paraneoplastic tissues surgically resected from patients and analyzed the expression of hsa_circ_0006421 in HCC tissues and its relationship with clinicopathological characteristics. Then, CCK8, colony formation, cell migration assay, transwell invasion assay and Annexin-V/PI staining were used to assess the effects of hsa_circ_0006421 on the growth, migration, invasion and apoptosis of HCC cells. Next, quantitative real-time PCR (qRT-PCR) and Western blots were used to detect the expression of miR-134-5p, CELF2 and hsa_circ_0006421. In the end, the targeting interactions of miR-134-5p and hsa_circ_0006421，CELF2 and miR-134-5p were explored by the dual-luciferase reporter assay.Results: Hsa_circ_0006421 was diminished in HCC tissues and its down-regulation was related to cirrhosis history. Knocking down hsa_circ_0006421 promoted HCC cells growth, migration, invasion and inhibited apoptosis, whereas overexpressing hsa_circ_0006421 had the opposite effects. Moreover, hsa_circ_0006421 served as the competing endogenous RNA of miR-134-5p. Subsequently, there was a reciprocal relationship between CELF2 and miR-134-5p. Hsa_circ_0006421 could positively regulate the protein level of CELF2 in HCC. Conclusion: hsa_circ_0006421 inhibits liver cancer by regulating miR-134-5p/CELF2 axis.

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Circular RNA circ_0004277 Inhibits Acute Myeloid Leukemia Progression Through MicroRNA-134-5p / Single stranded DNA binding protein 2

Cited by 18 publications

References 32 publications

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

Predicting the influence of Circ_0059706 expression on prognosis in patients with acute myeloid leukemia using classical statistics and machine learning

Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway

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