Circular RNA circ_0073181 contributes to tumorigenesis by regulating protein tyrosine phosphatase receptor type E via miR-548p in hepatocellular carcinoma

Abstract

Hepatocellular carcinoma (HCC) is a major world public problem in the world, with high morbidity and mortality rates. Circular RNA (circRNA) circ_0073181 has been reported to be related to HCC development. However, the mechanism of circ_0073181 in HCC is far from being addressed. Circ_0073181, microRNA-548p (miR-548p) and protein tyrosine phosphatase receptor type E (PTPRE) level were detected by real-time quantitative PCR (RT-qPCR). Cell proliferation, migration, invasion and apoptosis were detected by Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine, wound healing, transwell and flow cytometry assay. Protein levels of proliferating cell nuclear antigen, Bcl-2 related X protein (Bax) and PTPRE were examined by western blot assay. The binding relationship between miR-548p and circ_0073181 or PTPRE was predicted by circular RNA interactome and targetScan and then verified by a dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. The biologic role of circ_0073181 on HCC tumor growth was examined by the xenograft tumor model in vivo. Circ_0073181 and PTPRE were upregulated, and miR-548p was decreased in HCC tissues and cells. Furthermore, circ_0073181 knockdown could boost proliferation, migration, invasion and repress apoptosis of HCC cells in vitro. The mechanical analysis suggested that circ_0073181 could regulate PTPRE expression by sponging miR-548p. In addition, circ_0073181 knockdown suppressed cell growth of HCC in vivo. Circ_0073181 silencing could inhibit HCC cell growth and metastasis partly by regulating the miR-548p/ PTPRE axis, providing a promising therapeutic target for the HCC treatment.

Hepatocellular Carcinoma: Up-regulated Circular RNAs Which Mediate Efficacy in PreclinicalIn VivoModels

“…Circ0073181 up-regulates receptor tyrosine phosphatase epsilon (PTPRE). Circ0073181 (Figure 1) was up-regulated in tissues of HCC patients and corresponding cell lines (54). Knockdown of circ0073181 suppressed proliferation, migration, invasion and promoted apoptosis of HCC cell lines in vitro and cell growth and metastasis in nude mice.…”

“…Knockdown of circ0073181 suppressed proliferation, migration, invasion and promoted apoptosis of HCC cell lines in vitro and cell growth and metastasis in nude mice. Circ0073181 sponged miR-548p and up-regulated PTPRE (54). The latter is a transmembrane protein which is highly expressed in brain, testes, lymph nodes and lungs (55).…”

MicroRNA-1205 Suppresses Hepatocellular Carcinoma Cell Proliferation via a CSNK2B/CDK4 Axis

“…is also essential during the progression of HCC. [11][12][13] MiR-1205, a miRNA originally identified in a genomically unstable region of human chromosome 8q24, 14 has been reported to be abnormally expressed in multiple tumors (prostate cancer, colorectal cancer, glioma, etc. ), resulting in the inhibition or progression of these tumors.…”