Circular RNA circCCNB1 inhibits the migration and invasion of nasopharyngeal carcinoma through binding and stabilizing TJP1 mRNA

Zhao, Mengyao; Wang, Yian; Tan, Fenghua; Liu, Lingyun; Hou, Xiangchan; Tang, Le; Mo, Yongzhen; Wang, Yumin; Yan, Qijia; Gong, Zhaojian; Li, Zheng; Liao, Qianjin; Guo, Can; Huang, Hongdong; Zeng, Xi; Li, Guiyuan; Zeng, Zhaoyang; Xiong, Wei; Wang, Fuyan

doi:10.1007/s11427-021-2089-8

Cited by 14 publications

(8 citation statements)

References 55 publications

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“…RNA interaction has recently gained popularity as a research topic. Currently, circRNA have also been shown to possesses the ability to interact with speci c mRNA and affected them stability, which in turn regulates tumorigenesis and development [33,34]. However, the important role of circRNA that bind to speci c mRNA in HCC is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

A novel circulating RNA circELMOD3 functions as a tumor suppressor in hepatocellular carcinoma through TRIM13/P53 signal axis

Lai

Zeng

Liu

et al. 2022

Preprint

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Background Circular RNAs (circRNAs) have been identified as key regulatory factors in tumor development based on accumulating studies. However, the underlying molecular mechanisms of the circulating circRNAs in hepatocellular carcinoma (HCC) haven't t been fully elucidated. Methods Five pairs of HCC and adjacent normal tissues were processed using RNA-sequencing to determine the differential expressed circRNAs. The expression levels of genes and proteins were detected by qPCR and Western blotting, respectively. CCK-8, EdU, Flow cytometry, wound healing assay, Transwell assays, and xenograft mouse model were performed to investigate the biological function of circELMOD3 both in vitro and in vivo. Fluorescence in situ hybridization (FISH), RNA antisense purification (RAP) and dual luciferase reporter assay were carried out to verify the interaction between circELMOD3, miR-6864-5P and TRIM13. Results CircELMOD3 was downregulated in plasma and tissues from HCC patients and was related to their clinicopathological characteristics. Significantly, plasma circELMOD3 was shown to be a highly sensitive and non-invasive biomarker to distinguish HCC from healthy controls (AUC = 0.908). Functionally, circELMOD3 prevented HCC cells from proliferating and caused them to undergo apoptosis both in vitro and in vivo. Mechanistically, circELMOD3 increased the expression of TRIM13 by acting as a sponge for miR-6864-5P. In addition, overexpression of circELMOD3 lead to enhanced stability and higher expression level of TRIM13 mRNA, to which it directly binds, and in turn activated the P53 signaling pathway. Conclusion CircELMOD3 plays a tumor suppressor role in HCC via TRIM13/P53 signaling axis, which can serve as a potential target for early diagnosis and treatment of HCC patient.

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Section: Discussionmentioning

confidence: 99%

A novel circulating RNA circELMOD3 functions as a tumor suppressor in hepatocellular carcinoma through TRIM13/P53 signal axis

Lai

Zeng

Liu

et al. 2022

Preprint

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“…[18] CircCCNB1 was manifested to inhibit cell invasion and migration abilities of nasopharyngeal carcinoma. [19] Circ_100395 suppressed cell development and aerobic glycolysis in papillary thyroid cancer. [20] F Subsequently, the interaction between circ_PRDM5 and miR-25-3p in BC cells was affirmed.…”

Section: Circ_prdm5 Inhibited Bc Cell Malignant Progression By Target...mentioning

confidence: 99%

Circ_PRDM5/miR‐25‐3p/ANKRD46 axis is associated with cell malignant behaviors in subjects with breast cancer evaluated by ultrasound

Sun

et al. 2023

J Biochem & Molecular Tox

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Circular RNAs (circRNAs) are key RNA molecules in cancer biology. CircRNA PR/SET domain 5 (circ_PRDM5, hsa_circ_0005654) was downregulated in breast cancer (BC) tissues. This study is designed to investigate the functional mechanism of circ_PRDM5 in BC. Ultrasound examinations were performed to evaluate BC patients and normal individuals. Circ_PRDM5, miR‐25‐3p, and Ankyrin repeat domain 46 (ANKRD46) level detection was carried out by reverse transcription‐quantitative polymerase chain reaction. 3‐(4, 5‐dimethylthiazol‐2‐y1)‐2, 5‐diphenyl tetrazolium bromide (MTT) assay was used for cell viability examination. Cell proliferation was evaluated by ethynyl‐2′‐deoxyuridine assay and colony formation assay. The protein levels were examined using western blot. Cell migration and invasion abilities were assessed via transwell assay. Target interaction was analyzed via dual‐luciferase reporter assay. The role of circ_PRDM5 in vivo was explored via xenograft tumor assay. Circ_PRDM5 expression was downregulated in BC tissues and cells. Overexpression of circ_PRDM5 suppressed proliferation and motility but enhanced apoptosis of BC cells. Circ_PRDM5 served as a sponge of miR‐25‐3p. Circ_PRDM5 impeded BC cell malignant development via sponging miR‐25‐3p. Circ_PRDM5 induced ANKRD46 upregulation by targeting miR‐25‐3p. Inhibition of miR‐25‐3p retarded BC progression by increasing the ANKRD46 level. Circ_PRDM5 repressed BC tumorigenesis in vivo through mediating the miR‐25‐3p/ANKRD46 axis. This study evidenced that circ_PRDM5 inhibited cell progression and tumor growth in BC via interacting with mir‐25‐3p/ANKRD46 network.

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“…In addition to circRNA-000543, circ-CCNB1 has also been shown to be associated with EMT [ 109 ]. Circ-CCNB1 inhibits migration and invasion of nasopharyngeal carcinoma by promoting binding between NF90 and TJP1 mRNA, stabilizing TJP1 mRNA, and enhancing tight junctions between tumor cells.…”

Section: Research Progress Of Circrnas Related To Tumor Radioresistancementioning

confidence: 99%

CircRNAs in Tumor Radioresistance

Gao

Lin

et al. 2022

Biomolecules

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Circular RNAs (circRNAs) are endogenous, non-coding RNAs, which are derived from host genes that are present in several species and can be involved in the progression of various diseases. circRNAs’ leading role is to act as RNA sponges. In recent years, the other roles of circRNAs have been discovered, such as regulating transcription and translation, regulating host genes, and even being translated into proteins. As some tumor cells are no longer radiosensitive, tumor radioresistance has since become a challenge in treating tumors. In recent years, circRNAs are differentially expressed in tumor cells and can be used as biological markers of tumors. In addition, circRNAs can regulate the radiosensitivity of tumors. Here, we list the mechanisms of circRNAs in glioma, nasopharyngeal carcinoma, and non-small cell lung cancer; further, these studies also provide new ideas for the purposes of eliminating radioresistance in tumors.

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Circular RNA circCCNB1 inhibits the migration and invasion of nasopharyngeal carcinoma through binding and stabilizing TJP1 mRNA

Cited by 14 publications

References 55 publications

A novel circulating RNA circELMOD3 functions as a tumor suppressor in hepatocellular carcinoma through TRIM13/P53 signal axis

A novel circulating RNA circELMOD3 functions as a tumor suppressor in hepatocellular carcinoma through TRIM13/P53 signal axis

Circ_PRDM5/miR‐25‐3p/ANKRD46 axis is associated with cell malignant behaviors in subjects with breast cancer evaluated by ultrasound

CircRNAs in Tumor Radioresistance

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