Circular RNA circPRKDC promotes tumorigenesis of gastric cancer via modulating insulin receptor substrate 2 (IRS2) and mediating microRNA-493-5p

Liu, Zhipeng; Wang, Yangang; Ding, Yuanhua

doi:10.1080/21655979.2021.1981798

Cited by 8 publications

(3 citation statements)

References 42 publications

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“…The tissue expression profile analysis showed that the expression of bbu-miR-493-5p was significantly higher in buffalo skeletal muscle than in other organs = (heart, liver, spleen, lung), suggesting that it plays an important role in buffalo skeletal muscle development and is worth researching. miR-493-5p has been widely used as a tumor suppressor in various cancers, such as hepatocellular carcinoma [20,21], cervical cancer [22], renal cell carcinoma [23], gastric cancer [24], and so on. In recent years, miR-493-5p has also been found to be associated with skeletal muscle growth and development.…”

Section: Discussionmentioning

confidence: 99%

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Zhang,

Zhong,

Yao

et al. 2024

Animals

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In recent years, the meat and dairy value of buffaloes has become a major concern in buffalo breeding, and the improvement of buffalo beef quality is key to protecting buffalo germplasm resources and solving the problem of beef supply. MiRNAs play a significant role in regulating muscle development. However, the precise mechanism by which they regulate the development of buffalo skeletal muscles remains largely unexplored. In this study, we examined miRNA expression profiles in buffalo myoblasts during the proliferation and differentiation stages. A total of 177 differentially expressed miRNAs were identified, out of which 88 were up-regulated and 89 down-regulated. We focused on a novel miRNA, named bbu-miR-493-5p, that was significantly differentially expressed during the proliferation and differentiation of buffalo myoblasts and highly expressed in muscle tissues. The RNA-FISH results showed that bbu-miR-493-5p was primarily located in the cytoplasm to encourage buffalo myoblasts’ proliferation and differentiation. In conclusion, our study lays the groundwork for future research into the regulatory role of miRNAs in the growth of buffalo muscle.

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Section: Discussionmentioning

confidence: 99%

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Zhang,

Zhong,

Yao

et al. 2024

Animals

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“…Several studies have shown that miR‐493‐5p has antitumor functions in various cancer types. Although miR‐493‐5p has been reported to function in a suppressive manner in various cancer types, 27 , 28 , 29 it has not been reported in cholangiocarcinomas, including ICC. The expression patterns and biological mechanisms of miR‐493‐5p in ICC progression have not yet been fully elucidated.…”

Section: Discussionmentioning

confidence: 99%

Cancer‐associated fibroblasts promote tumor cell growth via miR‐493‐5p in intrahepatic cholangiocarcinoma

et al. 2022

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The association between tumor microenvironment (TME) and cancer‐associated fibroblasts (CAFs) in intrahepatic cholangiocarcinoma (ICC) progression is poorly understood. This study aimed to reveal whether specific microRNAs (miRNAs) in extracellular vesicles (EVs) derived from CAFs were involved in ICC progression. Conditioned medium (CM) and EVs in the CM of CAFs and normal fibroblasts (NFs) derived from ICC specimens were used to investigate the effects on tumor cell lines. miRNA microarray assay was used to examine the miRNAs of EVs derived from CAFs and NFs in ICC, and the effects of miR‐493‐5p on tumor cell lines were examined. Additionally, databases were used to identify miR‐493‐5p targets, and the relationship between prognosis of ICC patients and cocaine‐ and amphetamine‐regulated transcript propeptide (CARTPT), one of the targets of miR‐493‐5p, expression in ICC tissues was retrospectively analyzed. Compared with NF‐derived CM and EVs, CAF‐derived CM and EVs promoted cell lines in proliferation, scratch, migration, and invasion assays. miRNA microarray analysis revealed that miR‐493‐5p was significantly increased in CAF‐derived EVs compared to NF‐derived EVs. Tumor cell lines transfected with miR‐493‐5p were promoted in proliferation and scratch assays. Immunohistochemical staining was performed on 76 ICC specimens; both overall and recurrence‐free survival rates were significantly worse in the CARTPT‐negative group. Univariate and multivariate analyses showed that low CARTPT expression was an independent poor prognostic factor for overall and recurrence‐free survival. Overall, our data suggest that CAFs in the ICC TME suppress CARTPT in tumor cells and promote tumor cells via miR‐493‐5p in EVs.

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“…It has been observed that the expression and function of the IRS may vary in the different types of cancer. For example, IRS-1 has been associated with proliferation, growth, and anti-apoptosis, whereas IRS-2 has been linked to metastasis, motility, and invasion [ 22 , 23 , 24 , 25 , 26 ]. IRS proteins participate in canonical pathways, the phosphorylation of which is induced by the insulin receptor.…”

Section: Introductionmentioning

confidence: 99%

The Insulin Receptor Substrate 2 Mediates the Action of Insulin on HeLa Cell Migration via the PI3K/Akt Signaling Pathway

Báez

Romero²,

Chihu-Amparan

et al. 2023

CIMB

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Insulin signaling plays an important role in the development and progression of cancer since it is involved in proliferation and migration processes. It has been shown that the A isoform of the insulin receptor (IR-A) is often overexpressed, and its stimulation induces changes in the expression of the insulin receptor substrates (IRS-1 and IRS-2), which are expressed differently in the different types of cancer. We study the participation of the insulin substrates IRS-1 and IRS-2 in the insulin signaling pathway in response to insulin and their involvement in the proliferation and migration of the cervical cancer cell line. Our results showed that under basal conditions, the IR-A isoform was predominantly expressed. Stimulation of HeLa cells with 50 nM insulin led to the phosphorylation of IR-A, showing a statistically significant increase at 30 min (p ≤ 0.05). Stimulation of HeLa cells with insulin induces PI3K and AKT phosphorylation through the activation of IRS2, but not IRS1. While PI3K reached the highest level at 30 min after treatment (p ≤ 0.05), AKT had the highest levels from 15 min (p ≤ 0.05) and remained constant for 6 h. ERK1 and ERK2 expression was also observed, but only ERK2 was phosphorylated in a time-dependent manner, reaching a maximum peak 5 min after insulin stimulation. Although no effect on cell proliferation was observed, insulin stimulation of HeLa cells markedly promoted cell migration.

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Circular RNA circPRKDC promotes tumorigenesis of gastric cancer via modulating insulin receptor substrate 2 (IRS2) and mediating microRNA-493-5p

Cited by 8 publications

References 42 publications

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation

Cancer‐associated fibroblasts promote tumor cell growth via miR‐493‐5p in intrahepatic cholangiocarcinoma

The Insulin Receptor Substrate 2 Mediates the Action of Insulin on HeLa Cell Migration via the PI3K/Akt Signaling Pathway

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