Circular RNA Expression and Regulation Profiling in Testicular Tissues of Immature and Mature Wandong Cattle (Bos taurus)

Abstract

Wandong cattle are an autochthonous Chinese breed used extensively for beef production. The breed tolerates extreme weather conditions and raw feed and is resistant to tick-borne diseases. However, the genetic basis of testis development and sperm production as well as breeding management is not well established in local cattle. Therefore, improving the reproductive efficiency of bulls via genetic selection is crucial as a single bull can breed thousands of cows through artificial insemination (AI). Testis development and spermatogenesis are regulated by hundreds of genes and transcriptomes. However, circular RNAs (circRNAs) are the key players in many biological developmental processes that have not been methodically described and compared between immature and mature stages in Bovine testes. In this study, we performed total RNA-seq and comprehensively analyzed the circRNA expression profiling of the testis samples of six bulls at 3 years and 3 months of developmental age. In total, 17,013 circRNAs were identified, of which 681 circRNAs (p-adjust < 0.05) were differentially expressed (DE). Among these DE circRNAs, 579 were upregulated and 103 were downregulated in calf and bull testes. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that the identified target genes were classified into three broad functional categories, including biological process, cellular component, and molecular function, and were enriched in the lysine degradation, cell cycle, and cell adhesion molecule pathways. The binding interactions between DE circRNAs and microRNAs (miRNAs) were subsequently constructed using bioinformatics approaches. The source genes ATM, CCNA1, GSK3B, KMT2C, KMT2E, NSD2, SUCLG2, QKI, HOMER1, and SNAP91 were found to be actively associated with bull sexual maturity and spermatogenesis. In addition, a real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed a strong correlation with the sequencing data. Moreover, the developed model of Bovine testes in the current study provides a suitable framework for understanding the mechanism of circRNAs in the development of testes and spermatogenesis.

Identification and analysis of differentially expressed (DE) circRNA in epididymis of yak and cattleyak

“…In the analysis of circRNA expression characteristics of testicular samples from 6-month-old bulls, a total of 17,013 circRNAs were identified, of which 681 circRNAs were differentially expressed. Of these DE circRNAs, 579 were u p- regulated and 103 were down regulated in calf and bull testis ( 36 ). In consistent with previous reports, we identified that most of the circRNAs were derived from exonic regions, then intergenic and intronic regions, respectively.…”

Circular RNA regulates male spermatogenesis: a narrative review

“…Testing of some artificially synthesized circular RNAs revealed that they contain an internal ribosome entry site. These circular RNAs may contain functions that encode proteins [11].…”

Transcriptome sequencing reveals the effects of circRNA on testicular development and spermatogenesis in Qianbei Ma goats

“…CircRNAs are a prominent new class of closed-loop RNA molecules found in eukaryotes, that are produced by the alternate shearing of precursor mRNAs ( 7 ), and are involved in the regulation of mammalian development ( 8 ), growth ( 9 ), reproduction ( 10 ), and immunity ( 11 ) in animals. For example, circPPARA can act as an endogenous sponge to adsorb mir-429 and mir-200b , thereby promoting intramuscular adipogenesis in pigs ( 12 ), and reduced expression of circZNF609 significantly inhibits myoblast proliferation in mice in vivo ( 13 ).…”