Circular RNA expression in human hematopoietic cells is widespread and cell-type specific

Nicolet, Benoît P; Engels, Sander; Aglialoro, Francesca; Akker, Emile van den; Lindern, Marieke von; Wolkers, Monika C.

doi:10.1093/nar/gky721

Cited by 149 publications

(104 citation statements)

References 59 publications

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“…For example, approximately 15,000 circRNAs are expressed in both human and mouse orthologous loci, representing approximately 15% and 40% of the total cir-cRNAs in humans and mice, respectively [40]. (4) Specificity: in terms of cell type, tissue or developmental stage, circRNAs often show specific expression [41][42][43][44].…”

Section: Circrnasmentioning

confidence: 99%

Roles of circRNAs in the tumour microenvironment

et al. 2020

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The tumour microenvironment (TME) constitutes the area surrounding the tumour during its development and has been demonstrated to play roles in cancer-related diseases through crosstalk with tumour cells. Circular RNAs (circRNAs) are a subpopulation of endogenous noncoding RNAs (ncRNAs) that are ubiquitously expressed in eukaryotes and have multiple biological functions in the regulation of cancer onset and progression. An increasing number of studies have shown that circRNAs participate in the multifaceted biological regulation of the TME. However, details on the mechanisms involved have remained elusive until now. In this review, we analyse the effects of circRNAs on the TME from various perspectives, including immune surveillance, angiogenesis, hypoxia, matrix remodelling, exo-circRNAs and chemoradiation resistance. Currently, the enormous potential for circRNA use in targeted therapy and as noninvasive biomarkers have drawn our attention. We emphasize the prospect of targeting circRNAs as an essential strategy to regulate TME, overcome cancer resistance and improve therapeutic outcomes.

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Section: Circrnasmentioning

confidence: 99%

Roles of circRNAs in the tumour microenvironment

et al. 2020

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“…Circular RNAs (circRNAs) are a class of covalently closed single-stranded RNA molecules whose 3′ and 5′ ends are linked by a back-splice event ( 6 ). Some of them have been proven to be structurally stable ( 7 ), tissue and cell specific ( 8 ), abundantly expressed in various tissues and cells ( 9 ), and evolutionarily conserved ( 10 ). Emerging evidence has shown that some circRNAs are involved in many biological processes and a variety of human diseases, including innate immunity ( 11 ), inflammation ( 12 ), tumorigenesis ( 13 , 14 ), aging ( 15 ), diabetes mellitus ( 16 ), neurological disorders ( 17 ), and cardiovascular diseases ( 18 ) by acting as microRNA (miRNA) sponges ( 19 – 21 ), regulating splicing and transcription ( 22 , 23 ), interacting with RNA-binding proteins ( 24 , 25 ), or serving as protein translation templates ( 26 – 28 ).…”

Section: Introductionmentioning

confidence: 99%

Hsa_circ_0012152 and Hsa_circ_0001857 Accurately Discriminate Acute Lymphoblastic Leukemia From Acute Myeloid Leukemia

Guo

Chen

et al. 2020

Front. Oncol.

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Acute leukemia (AL) is a group of highly heterogeneous hematological malignancies. Circular RNAs (circRNAs) are covalently closed circRNA molecules implicated in the development of many diseases. However, the role of circRNAs in AL remains largely unknown. Therefore, this study aimed to identify new classification diagnostic biomarkers for subgroups of AL. The circRNA expression signatures discriminating acute lymphoblastic leukemia (ALL) from acute myeloid leukemia (AML) were identified by microarray, followed by reverse transcription quantitative polymerase chain reaction (RT-qPCR) validation. Receiver operating characteristic curve analysis was used to evaluate the diagnostic efficiencies of hsa_circ_0001857 and hsa_circ_0012152, and hsa_circ_0012152 was selected for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. The results showed that the circRNA expression profiles, hsa_circ_0001857, and hsa_circ_0012152 could clearly discriminate ALL from AML. The target genes of hsa_circ_0012152 might be involved in biological processes, such as myeloid cell differentiation, covalent chromatin modification, histone modification, and rat sarcoma (Ras) protein signal transduction, and participate in pathways such as mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3′-kinase (PI3K)-Akt signaling pathway. Hsa_circ_0012152 might be involved in the initiation and development of AML through miR-491-5p/epidermal growth factor receptor (EGFR)/MAPK1 or miR-512-3p/EGFR/MAPK1 axis. Our results showed that circRNA expression profiles and specifically expressed circRNAs were promising classification biomarkers to designate AL into ALL or AML.

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“…Next Generation Sequencing (NGS) recently showed a conspicuous circRNA expression in human hematopoietic progenitors, and in differentiated lymphoid and myeloid cells (Nicolet et al, 2018). In the hematopoietic compartment, circRNAs are significantly enriched and secreted in vesicles named exosomes derived from platelets, where circRNAs resulted to be more abundant and widely expressed compared with other cell types (Preußer et al, 2018).…”

Section: Circrnas In Hematopoiesismentioning

confidence: 99%

“…In the hematopoietic system, circRNA expression is cell-type specific, and it increases upon cell maturation. CircRNA splicing variants can also be cell-type specific (Nicolet et al, 2018). In the bone marrow, the hematopoietic stem cells (HSCs) differentiate into various progenitor cells, which in turn generate many types of myeloid and lymphoid cells requiring a tight regulation of gene expression of transcription factors and non-coding RNAs (Goode et al, 2016).…”

Section: Circrnas In Hematopoiesismentioning

confidence: 99%

Circular RNAs in Embryogenesis and Cell Differentiation With a Focus on Cancer Development

Agostino¹,

Riccioli

Cesaris

et al. 2020

Front. Cell Dev. Biol.

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In the recent years thousands of non-coding RNAs have been identified, also thanks to highthroughput sequencing technologies. Among them, circular RNAs (circRNAs) are a well-represented class characterized by the high sequence conservation and cell type specific expression in eukaryotes. They are covalently closed loops formed through back-splicing. Recently, circRNAs were shown to regulate a variety of cellular processes functioning as miRNA sponges, RBP binding molecules, transcriptional regulators, scaffold for protein translation, as well as immune regulators. A growing number of studies are showing that deregulated expression of circRNAs plays important and decisive actions during the development of several human diseases, including cancer. The research on their biogenesis and on the various molecular mechanisms in which they are involved is going very fast, however, there are still few studies that address their involvement in embryogenesis and eukaryotic development. This review has the intent to describe the most recent progress in the study of the biogenesis and molecular activities of circRNAs providing insightful information in the field of embryogenesis and cell differentiation. In addition, we describe the latest research on circRNAs as novel promising biomarkers in diverse types of tumors.

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Circular RNA expression in human hematopoietic cells is widespread and cell-type specific

Cited by 149 publications

References 59 publications

Roles of circRNAs in the tumour microenvironment

Roles of circRNAs in the tumour microenvironment

Hsa_circ_0012152 and Hsa_circ_0001857 Accurately Discriminate Acute Lymphoblastic Leukemia From Acute Myeloid Leukemia

Circular RNAs in Embryogenesis and Cell Differentiation With a Focus on Cancer Development

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