Circular RNA expression profiling in dorsal root ganglion of rats with peripheral nerve injury-induced neuropathic pain

Abstract

Background : Circular RNAs (circRNAs) comprise a class of endogenous species of RNA consisting of a covalently closed loop structure that is crucial for genetic and epigenetic regulation. The significance of circRNA in neuropathic pain remains to be investigated. Methods : The sciatic nerve chronic constriction injury (CCI) model was established to induce neuropathic pain. We performed genome-wide circRNA analysis of 4 paired DRG sample from CCI and NC rats via next generation sequencing technology. The differentially expressed circRNAs (DEcircRNAs) were identified by differential expression analysis and the expression profile of circRNAs was validated by quantitative real-time PCR (qPCR). Functional annotation analysis was performed to predict the function of DEcircRNAs. Results : A total of 374 DEcirRNAs were identified between CCI and NC rats using circRNA High-throughput sequencing (HTS). Expression levels of 9 DEcircRNAs were validated by qPCR. Functional annotation analysis showed that DEcircRNAs were mainly enriched in pathways and functions such as ‘dopaminergic synapse’, ‘renin secretion’, ‘MAPK signaling pathway’ and ‘neurogenesis’. Competing endogenous RNAs analysis showed that top 50 circRNAs exhibited interactions with four pain related miRNAs. Circ:chr2:33950934-33955969 is the largest node in the circRNA-miRNA interaction network. Conclusion : DEcircRNAs may advance our understanding of the molecular mechanisms underlying neuropathic pain. Key words : neuropathic pain, circRNA, CCI, differential expression analysis

DNA Methylation and Non-Coding RNAs during Tissue-Injury Associated Pain

“…They offer a higher degree of specificity and sensitivity and have recently become more accessible and cost-effective. With this technique, Xiong and colleagues were able to identify a total of 374 circRNA differentially expressed in the DRG of rats following the induction of neuropathic pain by CCI (preprint here: [ 55 ]). This approach has also been used to identify differentially expressed miRNA, lncRNAs and circRNAs in the spinal cord of a model of diabetic neuropathic pain [ 59 ]; lncRNAs and mRNA in the spinal cord of rats subjected to paclitaxel-induced peripheral neuropathy [ 52 ]; and miRNA and mRNA in the DRG of rats between a CCI group and a CCI-exercise group [ 73 ].…”