Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis

Abstract

Circular RNAs (circRNAs) are known to act as key regulators in a variety of malignancies. However, the role of circRNAs in cervical cancer (CCa) remains largely unknown. Herein, we demonstrated that a circRNA derived from the TADA2A gene (hsa_circ_0043280) was significantly downregulated in CCa and that this reduction in expression was correlated with a poor prognosis. Furthermore, our results demonstrated that hsa_circ_0043280 functions as a tumor suppressor to inhibit tumor growth and metastasis in CCa. Mechanistically, hsa_circ_0043280 competitively sponges miR-203a-3p and prevents miR-203a-3p from reducing the levels of PAQR3. Collectively, our results demonstrate that hsa_circ_0043280 plays a pivotal role in the development and metastasis of CCa, thus suggesting that hsa_circ_0043280 has significant potential as a prognostic biomarker and a therapeutic target for CCa.

Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2)

“…Circ_0003204 overexpression resulted in CC cell growth and invasion promotion [ 35 ]. Differently, circSmarca5 and circ_0043280 served as cancer inhibitors for CC development [ 36 , 37 ]. In this study, we detected the significant upregulation of circ_0008460 in CC.…”

CircVPRBP Inhibits Nodal Metastasis of Cervical Cancer by Impeding RACK1 O-GlcNAcylation and Stability

“…Our previous study has identified circVPRBP (hsa_circ_0065898) as a 367-base circRNA down-regulated in CCa [27], as sanger sequence showed the back splicing site of the circVPRBP (Fig. 1A).…”

“…qRT-PCR, RT-PCR, gel electrophoresis, IHC, western blotting, and HE staining, were performed as described previously [5,27,42]. The primary antibodies used in this study are given in Supplementary Table S4.…”

“…Accumulating evidence indicates that circRNAs may involve in the progression of many cancers, including breast cancer [24], glioblastoma [25], cervical cancer [26], et.al. We have previously reported that circular RNA hsa_circ_0043280 could inhibit cervical cancer LN metastasis through miR-203a-3p/PAQR3 axis [27]. Circular RNAs could function as miRNAs sponge or protein scaffold which led to the regulation of genes expression involved in CCa progression [28][29][30].…”

Research Progress of circRNAs in Glioblastoma

“…CircRNAs are rich in miRNAs binding sites and competitively repress transcriptional regulation of miRNAs, a new class of highly expressed and stable ceRNAs ( Shi et al, 2013 ). The cyclic RNA hsa_circ_0043280 can reduce PAQR3 levels by competitively absorbing miR-203a-3p and blocking miR-203a-3p, and can function as a tumor suppressor to inhibit tumor growth and metastasis in cervical cancer ( Zhang et al, 2021b ). Hsa_circ_0006349 promotes MKP1 expression through uptake of miR-98, which enhances proliferation and glycolysis of non-small-cell lung cancer (NSCLC) cells and promotes malignant progression of tumors ( Qin et al, 2021 ).…”