Circular RNA hsa_circ_0075828 Promotes Bladder Cancer Cell Proliferation Through Activation of CREB1

Abstract

Circular RNAs (circRNAs), one kind of non-coding RNA, have been reported as critical regulators for modulating gene expression in cancer. In this study, microarray analysis was used to screen circRNA expression profiles of bladder cancer (BC) 5637 cells, T24 cells and normal control SV-HUC-1 cells. The data from the microarray showed that hsa_circ_0075828 (named circCASC15) was most highly expressed in 5637 and T24 cells. circCASC15 was highly expressed in BC tissues and cells. Overexpression of circCASC15 was closely associated with BC tumor stage and promoted cell proliferation significantly in vitro and in vivo. Mechanistically, circCASC15 could act as miR-1224-5p sponge to activate the expression of CREB1 to promote cell proliferation in BC. In short, circCASC15 promotes cell proliferation in BC, which might be a new molecular target for BC diagnosis and therapy. [BMB Reports 2020; 53(2): 82-87] BMB Rep. 2020; 53(2): 82-87 www.bmbreports.org hsa_circ_0075828 promotes proliferation in BC Chengle Zhuang, et al.

Progression Risk Assessment of Post-Surgical Papillary Thyroid Carcinoma Based on Circular RNA-Associated Competing Endogenous RNA Mechanisms

“…The luciferase reporter assay con rmed that hsa_circ_0089153 sponged hsa-miR-145 to mediate upregulation of ZEB2 expression, thereby playing a carcinogenic role in PTC. hsa_circ_0089153 was also reported to be upregulated in gastric cancer and bladder cancer [25,26]. Our study indicated that upregulated hsa_circ_0089153 may sponge hsa-miR-139-3p, and upregulated SPC24 and CDKN2A expression to promote PTC progression.…”

<p>Circular RNAs and Bladder Cancer</p>

“…Because clinicopathological characteristics affect the prognosis of patients and circRNAs are closely related to the clinicopathological characteristics of BC, circRNAs may also be related to the prognosis of patients. Cumulative studies have confirmed that circ-BPTF, circTFRC, circZFR and circCASC15 are associated with overall survival in patients with BC; 27,28,30,34 circZFR, circPRMT5 and circCASC15 are associated with diseasefree survival; 30,31,34 and hsa_circ_0137439 is associated with recurrence-free survival and overall survival. 35 In general, higher levels of these circRNAs are associated with less favorable patient survival.…”

“…Further analysis showed that high levels of these circRNAs are closely related to the clinicopathological stage of BC, which describes the tumor status, including the tumor size, presence of local and distant metastasis, and tumor pathological grade, and affect the survival of BC patients. [27][28][29][30][31][32][33][34] Regarding tumor tissue classifications, the levels of circRNAs, including circ-BPTF, circTFRC, circZFR, circPTK2, and circASXL1, were found to be significantly higher in low-grade BC tissues than in high-grade BC tissues. 27,28,30,32,33 Regarding the pathological TNM stage, circRNAs, including circTFRC, circZFR, circPRMT5, circPTK2, circASXL1 and circCASC15, were positively correlated with the T stage when patients were stratified into Ta-1 and T2-4 subgroups.…”

“…27,28,30,32,33 Regarding the pathological TNM stage, circRNAs, including circTFRC, circZFR, circPRMT5, circPTK2, circASXL1 and circCASC15, were positively correlated with the T stage when patients were stratified into Ta-1 and T2-4 subgroups. 28,[30][31][32][33][34] Additionally, circTFRC, circZFR, circPRMT5, circPTK2 and circASXL1 were found to be closely related to lymph node status: BC patients with lymph node metastasis tended to have higher levels of these circRNAs. 28,[30][31][32][33] In addition, circASXL1 is associated with distant metastasis, and patients with BC with distant metastasis have higher levels of circASXL1.…”

“…126,127 CircRNA_0058063 (hsa_circ_0058063) directly interacts with miR-486-3p to regulate the levels of the FOXP4 transcription factor, and circCASC15 promotes the activation of CREB1 by sponging miR-1224-5p. 34,128 Low expression of circFAM114A2 in BC weakens miR-762mediated inhibition of ΔNP63, a major isomer of the transcription factor TP63. 129 High expression of hsa_-circ_0000144 in BC increases its direct binding to miR-217, thereby abrogating its inhibitory effect on the transcription factor RUNX2 and subsequently increasing RNX2 expression.…”