Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p

Kong, Zhe; Wan, Xuechao; Lu, Yu‐Chuan; Zhang, Yingyi; Huang, Yan; Xu, Yali; Liu, Yajuan; Zhao, Peiqing; Xiang, Xinxin; Li, Liang; Yao, Li

doi:10.1111/jcmm.14791

Cited by 116 publications

(114 citation statements)

References 31 publications

Supporting

Mentioning

110

Contrasting

Order By: Relevance

“…Circ 000984 promotes cell proliferation and metastasis in NSCLC by modulating Wnt/β-catenin pathway [23]. CircRNA circFOXO3 promotes the prostate cancer progression [24]. The down-regulation of circRNA ciRS-7 [25] or circ 0067934 [26] blocks cell proliferation, migration and invasion of NSCLC cells.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

et al. 2020

View full text Add to dashboard Cite

Objective: Non-small cell lung cancer (NSCLC) continues to top the list of cancer mortalities worldwide. Early diagnosis and therapeutic interventions targeting NSCLC is becoming the world's significant challenge. Circular RNAs (circRNAs) are emerging as a group of potential cancer biomarkers. Materials and methods: Quantitative real-time PCR (qRT-PCR) was employed to examine the expression of circ 0072309 in NSCLC tissues and cell lines. Cell counting kit 8 (CCK-8), wound healing and Transwell assays were used to analyze cell proliferation, migration and invasion in A549 and H1299 cells. The relationship between circ 0072309 and miR-580-3 was analyzed by Luciferase reporter and RNA pull down assays. Results: We screened circ 0072309 from Gene Expression Omnibus and found that circ 0072309 was lowly expressed in NSCLC tissues and cell lines. The transfection of circ 0072309-overexpressing vector significantly suppressed the cell proliferation, migration and invasion in A549 and H1299 cells. We predicted that miR-580-3p is a target of circ 0072309 by using publicly available bioinformatic algorithms Circinteractome tool and confirmed that circ 0072309 directly bound to miR-580-3p. Furthermore, the addition of miR-580-3p mitigated the blockage of cell proliferation, migration and invasion induced by circ 0072309. Conclusions: These data showed that circ 0072309 inhibits the progression of NSCLC progression via blocking the expression of miR-580-3p. These findings revealed the anti-tumor role of circ 0072309 during the development of NSCLC and provided a novel diagnostic biomarker and potential therapy for NSCLC.

show abstract

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Kong et al disclosed that circFOXO3 acted as a sponge of miR-29a-3p to facilitate prostate cancer cell proliferation and repressed apoptosis. 27 In this paper, the influences of circ-ZNF652 silencing on HCC cell growth, metastasis and glycolysis were all weakened by the inhibition of miR-29a-3p, indicating that circ-ZNF652 could sponge miR-29a-3p to modulate HCC progression. In HCC, miR-29a-3p could hamper cell growth and migration by targeting IGF1R.…”

Section: Discussionmentioning

confidence: 67%

Exosomal Circ-ZNF652 Promotes Cell Proliferation, Migration, Invasion and Glycolysis in Hepatocellular Carcinoma via miR-29a-3p/GUCD1 Axis

Li¹,

Zang²,

Zhang³

et al. 2020

CMAR

View full text Add to dashboard Cite

Background: Circular RNAs (circRNAs) play a crucial role in hepatocellular carcinoma (HCC) progression. However, the role of exosomal circRNAs in HCC is still largely unknown. We aimed to explore the function of exosomal circ-ZNF652 in HCC. Methods: The morphology and size of exosomes were examined by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). The expression of circ-ZNF652, ZNF652 mRNA, microRNA-29a-3p (miR-29a-3p) and guanylyl cyclase domain containing 1 (GUCD1) mRNA was determined by quantitative real-time polymerase chain reaction (qRT-PCR). The protein levels of CD63, CD81, hexokinase 2 (HK2) and GUCD1 were examined via Western blot assay. The stability of circ-ZNF652 was examined by RNase R digestion assay. Cell proliferation was analyzed by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Cell migration and invasion were assessed by transwell assay. The glycolysis level was detected via specific kits. The association between miR-29a-3p and circ-ZNF652 or GUCD1 was analyzed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. A murine xenograft model was constructed to explore the effect of circ-ZNF652 in vivo. Results: Exosomal circ-ZNF652 was upregulated in HCC patients' serums and HCC cells. Exosomal circ-ZNF652 could transfer to HCC cells, and circ-ZNF652 silencing suppressed HCC cell proliferation, migration, invasion and glycolysis. Circ-ZNF652 was a sponge of miR-29a-3p, and the inhibitory effect of circ-ZNF652 silencing on HCC cell progression was weakened by miR-29a-3p inhibitor. GUCD1 was a target gene of miR-29a-3p, and GUCD1 overexpression restored the effect of miR-29a-3p on HCC cell development. Moreover, circ-ZNF652 knockdown repressed tumor growth in vivo. Conclusion: Exosomal circ-ZNF652 contributes to HCC cell proliferation, migration, invasion and glycolysis by miR-29a-3p/GUCD1 axis.

show abstract

“…Although previous studies (Chen et al, 2017;Meng et al, 2017;Kristensen et al, 2018) have reported circRNA miRNA sponge functions in many types of cancers, little is known about the role of circRNAs in PCa. Kong et al (2020) showed that hsa_circ_0006404 promoted PCa progression through sponging miR-29a-3p, while Cai et al (2019) demonstrated that circHIPK3 overexpression accelerated the proliferation and invasion of PCa cells by regulating miRNA-339-3p. Additionally, Wang et al (2019) revealed that circRNA ITCH suppressed PCa progression by upregulating HOXB13 expression through sponging miR-17-5p.…”

Section: Discussionmentioning

confidence: 99%

Identification of Prostate Cancer-Related Circular RNA Through Bioinformatics Analysis

Lin

Chen

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

Background: Prostate cancer (PCa) is one of the most common malignant tumors worldwide. Accumulating evidence has suggested that circular RNAs (circRNAs) are involved in the development and progression of various cancers, and they show great potential as novel biomarkers. However, the underlying mechanisms and specific functions of most circRNAs in PCa remain unknown. Here, we aimed to identify circRNAs with potential roles in PCa from the PCa expression profile. Methods: We used data downloaded from the Gene Expression Omnibus to identify circRNAs that were differentially expressed between PCa samples and adjacent nontumor samples. Relative expression levels of identified circRNAs were validated by quantitative real-time PCR. Micro (mi)RNA response elements were predicted by the CircInteractome database, and miRNA target genes were predicted by miRDB, miRTarBase, and TargetScan databases. Gene ontology (GO) enrichment analysis and pathway analysis revealed the potential biological and functional roles of these target genes. A circRNA-miRNA-mRNA interaction network was constructed by Cytoscape. The interaction between circRNAs and miRNAs in PCa was thoroughly reviewed in the PubMed. Finally, the mRNA expression of these genes was validated by the Cancer Genome Atlas (TCGA) and Gene Expression Profiling Interactive Analysis (GEPIA) databases. The expression of proteins encoded by these genes was further validated by the Human protein Atlas (HPA) database. Results: A total of 60 circRNAs that were differentially expressed between PCa and healthy samples were screened, of which 15 were annotated. Three circRNAs (hsa_circ_0024353, hsa_circ_0085494, hsa_circ_0031408) certified the criteria were studied. The results of quantitative real-time PCR demonstrated that the expression of hsa_circ_0024353 was significantly downregulated in PC-3 cells when compared with RWPE-1 cells, while the expression of hsa_circ_0031408 and hsa_circ_0085494 was significantly upregulated in PC-3 cells when compared with RWPE-1 cells. GO and Kyoto Encyclopedia of Genes and Genomes analyses found that target genes were mainly enriched in metabolic processes and pathways involving phosphoinositide 3-kinase-Akt signaling, hypoxia-inducible factor-1 signaling, p53 signaling, and the cell cycle. A total of 11 miRNA target genes showing differential expression between

show abstract

Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p

Cited by 116 publications

References 31 publications

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p

<p>Exosomal Circ-ZNF652 Promotes Cell Proliferation, Migration, Invasion and Glycolysis in Hepatocellular Carcinoma via miR-29a-3p/GUCD1 Axis</p>

Identification of Prostate Cancer-Related Circular RNA Through Bioinformatics Analysis

Contact Info

Product

Resources

About