Circular RNA Involved in the Protective Effect of Malva sylvestris L. on Myocardial Ischemic/Re-Perfused Injury

Abstract

Ischemic heart disease has become a major health challenge worldwide. Malva sylvestris L. (MS) is a traditional herbal medicine with anti-inflammatory properties and have been used as antioxidant and anti-inflammatory agent in infectious diseases and inflammatory diseases.In this study, we aimed at elucidating the mechanism of MS against ischemia-reperfusion (I/R)-induced injury in vivo and in vitro. The I/R animal model in rats and oxygen glucose deprivation/re-oxygenation (OGD/Re) model in H9c2 cells were used in this study. MS was used to pre-treat the rats and cells. Electrocardiogram, histology staining, qPCR, ELISA, CCK-8, and circRNA microarray were performed. We found that pre-treatment with MS extract attenuate OGD/Re-induced cell apoptosis and cell viability inhibition in H9c2 cells. In addition, pre-treatment with MS protected against I/R injury in vivo. The protective effects of MS pre-treatment were associated with inflammatory genes expression and cytokines release. Further mechanistic investigation revealed that MS protected cardiomyocytes through regulating circular RNA (circRNA). We identified a novel circRNA circ003593 that mediated the protective role of MS in vitro through NLRP3 complex, which was associated with reperfusion injury salvage kinase (RISK) signaling pathway. Conclusion: this study is the first time to demonstrate the protective role of MS on I/R injury. Our findings reveal a novel circRNA circ003593-mediated the protective role of MS through NLRP3 inflammasome. Circ003593 may serve as a potential therapeutic target for ischemic heart diseases.

Top Edible Wild Plants of Eastern Mediterranean Region Part II: Anti-inflammatory Activity

“…Malva sylvestris is one of the most studied, published, and reviewed plants [for example, [112][113][114]. In our presentation, we have cited 19 publications of the anti-inflammatory activity of this plant [16,[54][55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71]. Phenolic compounds are among the top anti-inflammatory NPs contained in M. sylvestris, and malvidin-3,5-glucoside is the most active (Fig.…”

“…[64] The 95% aqueous methanol leaves extract was prepared and used to treat ischemia-reperfusion injury in rats (in vivo) and in H9c2 cells (in vitro). [65] Aerial parts were extracted with ethanol and fractionized with water. This fraction decreased the release of inflammatory biomarkers, especially IL-6 in HIV infected TZM-b1 cells.…”