Circular RNA KIF4A promotes cell migration, invasion and inhibits apoptosis through miR-152/ZEB1 axis in breast cancer

Abstract

Background Circular RNAs (circRNAs) have been demonstrated to exert crucial mediators in tumor initiation and development. Nevertheless, the roles of circKIF4A in breast cancer (BC) are still not very clear. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to determine the expression of circKIF4A, miR-152, zinc finger E-box binding homeobox 1 (ZEB1) mRNA and caspase-3. Western blot assay was utilized to examine the protein level of ZEB1. Transwell assay and flow-cytometric analysis were adopted for the evaluation of cell migration, invasion and apoptosis, respectively. The associations among circKIF4A, miR-152 and ZEB1 were predicted by online websites and verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Results CircKIF4A and ZEB1 were conspicuously upregulated and miR-152 was markedly reduced in BC tissues and cells. Deficiency of circKIF4A repressed migration, invasion and induced apoptosis of BC cells. Moreover, circKIF4A was confirmed to be a sponge of miR-152 and miR-152 could bind to ZEB1. MiR-152 inhibition or ZEB1 overexpression abolished the impacts of circKIF4A knockdown on cell migration, invasion and apoptosis in BC. Conclusion Silencing of circKIF4A hampered cell metastasis and promoted apoptosis by regulating ZEB1 via sponging miR-152 in BC.

CircRNA DNA methyltransferase 1 silence inhibits breast cancer development by regulating micoRNA‐485‐3p/zinc finger E‐box binding homeobox 1 axis

“…Liang et al and Fang et al suggested ZEB1 promoted breast cancer cell proliferation and invasion 16,27 . Moreover, Jin et al suggested that ZEB1 could facilitate breast cancer cell migration and invasion and restrain apoptosis 17 . In addition, ZEB1 could contribute to breast cancer cell proliferation, migration, and invasion by activating the protein kinase B (Akt) pathway 28 .…”

“…ZEB1 is a main member of ZEB family, which contributes to tumorigenic progression 15 . Furthermore, ZEB1 acts as a target of miR‐1236‐3p and miR‐152 to increase cell proliferation, migration, and invasion and inhibit apoptosis in breast cancer 16,17 . However, whether ZEB1 is interacted with miR‐485‐3p is unclear.…”

CircKIF4A Enhances Osteosarcoma Proliferation And Metastasis By Sponging MiR-515-5p And Upregulating SLC7A11

“…CircRNAs are highly expressed in cells and tissues, and are potential miRNA sponges for regulating the expressions of various key genes closely relatively to malignant tumor [22]. circKIF4A is signi cant in malignant progression of a variety of tumors [8][9][10][11][12][13]. We tested circKIF4A levels in OS cells and tissues.…”

Characterization of transcriptome diversity and in vitro behavior of primary human high-risk breast cells

“…Because our study combined transcriptional profiling with biological behavior in primary cell culture, we utilized Gene Set Enrichment Analysis (GSEA) 31 and the Molecular Signatures Database 32 , 33 for associations and patterns between gene expression and behavior. Previous research shows that individual gene expression patterns can be linked to specific cell behaviors including viability 34 – 39 and estrogen response 40 , 41 .…”