Circular RNA MAT2B promotes migration, invasion and epithelial-mesenchymal transition of non-small cell lung cancer cells by sponging miR-431

Wang, Hui; Li, Feng; Cheng, Deyun; Zheng, Yuqiong; Xie, Yimin; Fu, Bing

doi:10.1080/15384101.2021.1956106

Cited by 9 publications

(5 citation statements)

References 20 publications

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“… 20 Herein, we found that knockdown of circMMP1 in ESCC cells distinctly increased E‐cad level and decreased the levels of N‐cad and vimentin, suggesting that circMMP1 participated in the migration and invasion process of ESCC cells by regulating the EMT process. Furthermore, a number of circRNAs have been shown to promote cancer metastasis through influencing EMT, such as circMAT2B (non‐small cell lung cancer), 21 circ_0092314 (pancreatic cancer) 22 and circPTPN22 (gastric cancer). 23 …”

Section: Discussionmentioning

confidence: 99%

MiR‐671‐5p sponging activity of circMMP1 promotes esophageal squamous cancer progression

Li,

Chai,

Lei

et al. 2023

Thoracic Cancer

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BackgroundThe aim of this study was to explore the function and mechanism of circular RNA (circRNA) matrix metallopeptidase 1 (circMMP1) in the progression of esophageal squamous cell carcinoma (ESCC).MethodsCircMMP1 expression was detected by quantitative real‐time PCR (qRT‐PCR), and its relationship with the prognosis of ESCC patients was evaluated by Kaplan–Meier analysis. Cells were transfected using corresponding plasmids, and the cell proliferation activity, migration and invasion capabilities in vitro were assessed. The protein level in tissues and cells was analyzed using western blotting. RNA pulldown, dual‐luciferase reporter assay and RNA immunoprecipitation assay were performed in ESCC cells to detect the interaction between circMMP1 and miR‐671‐5p, or the correlation between miR‐671‐5p and ANO1. Xenograft tumor experiment was carried out to uncover the function of circMMP1 in vivo.ResultsThe high level of circMMP1 in tumor tissues was associated with poor prognoses of ESCC patients. Knockdown of circMMP1 suppressed ESCC cell proliferation, migration and invasion in vitro. MiR‐671‐5p was the target of circMMP1 and mediated the inhibition effect of circMMP1 on ESCC cells. CircMMP1 targeted miR‐671‐5p to regulate ANO1 expression, which was downstream of miR‐671‐5p. Overexpression of ANO1 weakened tumor‐repressive function of circMMP1 knockdown in ESCC cells. Moreover, silencing of circMMP1 impeded ESCC tumor growth in vivo.ConclusionOur study provided novel evidence that circMMP1 accelerated ESCC progression by acting as a miR‐671‐5p sponge to enhance ANO1 expression.

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Section: Discussionmentioning

confidence: 99%

MiR‐671‐5p sponging activity of circMMP1 promotes esophageal squamous cancer progression

Li,

Chai,

Lei

et al. 2023

Thoracic Cancer

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“…In several types of cancer, most lncRNAs are either up- or downregulated. These lncRNAs favor all stages of tumor development through the promotion of mRNA expression and constancy [ 253 ], by favoring mRNA stability [ 254 ], or by modulating miR [ 254 , 255 , 256 , 257 , 258 , 259 , 260 , 261 , 262 , 263 , 264 , 265 , 266 , 267 , 268 , 269 , 270 , 271 , 272 , 273 , 274 , 275 , 276 , 277 , 278 , 279 , 280 , 281 , 282 , 283 , 284 , 285 , 286 , 287 , 288 , 289 , 290 , 291 , 292 , 293 , 294 , 295 , 296 , 297 , 298 , 299 , 300 ]. The aberrant phenotype is the result of the modulation of typical pathways playing key roles in cell survival [ 255 , 259 , 263 , 265 , 283 ], apoptosis [ <...…”

Section: Analyzing Cernets For Diagnosis and Targeting Them For Thera...mentioning

confidence: 99%

Using ncRNAs as Tools in Cancer Diagnosis and Treatment—The Way towards Personalized Medicine to Improve Patients’ Health

Piergentili

Basile

Nocella

et al. 2022

IJMS

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Although the first discovery of a non-coding RNA (ncRNA) dates back to 1958, only in recent years has the complexity of the transcriptome started to be elucidated. However, its components are still under investigation and their identification is one of the challenges that scientists are presently facing. In addition, their function is still far from being fully understood. The non-coding portion of the genome is indeed the largest, both quantitatively and qualitatively. A large fraction of these ncRNAs have a regulatory role either in coding mRNAs or in other ncRNAs, creating an intracellular network of crossed interactions (competing endogenous RNA networks, or ceRNET) that fine-tune the gene expression in both health and disease. The alteration of the equilibrium among such interactions can be enough to cause a transition from health to disease, but the opposite is equally true, leading to the possibility of intervening based on these mechanisms to cure human conditions. In this review, we summarize the present knowledge on these mechanisms, illustrating how they can be used for disease treatment, the current challenges and pitfalls, and the roles of environmental and lifestyle-related contributing factors, in addition to the ethical, legal, and social issues arising from their (improper) use.

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“…Subsequently, blots were washed with Tris-based saline-Tween 20 for 30-40 minutes, then further probed with secondary antibody at RT for 1 hour and subjected to reaction with enhanced chemiluminescence solution and visualized using ChemiDoc XRS+ system (BioRad Laboratories Inc., Hercules, CA, USA). For quanti cation of speci c protein bands, lms were scanned and analyzed using Labworks software (Mission Viejo, CA, USA) (30,31).…”

Section: Western Blot Analysismentioning

confidence: 99%

Development of a Prognostic Model Based on Differentially Expressed Immune Genes of Lung Adenocarcinoma

Zhai¹,

Li²,

Wang³

et al. 2021

Preprint

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Due to the enormous heterogeneity and molecular complexity, the efficacy of existing lung adenocarcinoma risk prediction models were less than satisfactory. In this study, we downloaded the immune-related genes were from InnateDB, and the differentially expressed immune genes (DEIGs) were analyzed with edgeR and DESeq2 algorithm. In total, 1359 DEIGs were identified. Cytoscape was employed to build a mRNA-miRNA-lncRNA network which was consists of 8 lncRNAs, 7 miRNAs and 117 DEIGs. Functional enrichment analysis indicated that the 117 DEIGs were involved in immune and inflammatory responses and actively involved in a MAPK signaling pathway. A prognostic signature based on ten DEIGs including ASPH, CAV1, FKBP4, GRIK2, FURIN, SLC6A8, FSCN1, CKAP4, HAPLN2 and IL22RA2 which performed well was correlated with tumor burden, tumor stage and metastasis. The similar result was obtained in the validation dataset GSE72094 (p<0.0001). The prognostic index reflected infiltration by B cell, CD4+T cell, CD8+T cell and dendritic cell. We also found that ASPH and FSCN1 were over-expression in A549, H1299, PC-9 cells, and the positive expression of ASPH, FSCN1, MS4A1 and CD40LG had a correlation with the TNM stage, cellular differentiation and the lymphnode metastasis(p<0.05). High levels of ASPH and FSCN1, low levels of MS4A1 and CD40LG expression are all associated with poor overall survival in LUAD (p<0.05). In conclusion, our results identified DEIGs of clinical significance, verified the effect of the DEIGs-based, personalized prediction model for lung adenocarcinoma prognosis. Together, our results revealed that ASPH and FSCN1 could be a prognostic marker for lung adenocarcinoma.

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Circular RNA MAT2B promotes migration, invasion and epithelial-mesenchymal transition of non-small cell lung cancer cells by sponging miR-431

Cited by 9 publications

References 20 publications

MiR‐671‐5p sponging activity of circMMP1 promotes esophageal squamous cancer progression

MiR‐671‐5p sponging activity of circMMP1 promotes esophageal squamous cancer progression

Using ncRNAs as Tools in Cancer Diagnosis and Treatment—The Way towards Personalized Medicine to Improve Patients’ Health

Development of a Prognostic Model Based on Differentially Expressed Immune Genes of Lung Adenocarcinoma

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