Circular RNA MYLK as a competing endogenous RNA promotes bladder cancer progression through modulating VEGFA/VEGFR2 signaling pathway

Zhong, Zhenyu; Huang, Mengge; Lv, Mengxin; He, Yong; Duan, Changzhu; Zhang, Luyu; Chen, Junxia

doi:10.1016/j.canlet.2017.06.027

Cited by 396 publications

(366 citation statements)

References 29 publications

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“…Additionally, previous researches revealed that circRNA‐MYLK functioned as an endogenous sponge for miR‐29a in bladder cancer. Whereas circRNA‐MYLK knockdown decreased cell proliferation, motility and induced apoptosis . Herein, circCEP128 served as a sponge for miR‐145‐5p, further increased cell proliferation, motility and inhibited apoptosis.…”

Section: Discussionmentioning

confidence: 94%

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Sun

Zhao

Chen

et al. 2019

Intl Journal of Cancer

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The present experiment was designed for exploring the regulatory mechanism of circ‐CEP128/miR‐145‐5p/MYD88 axis in bladder cancer. MiRNAs and circRNAs expression data were derived from Gene Expression Omnibus database with bladder tumor tissues and paracarcinoma tissue samples. Differentially expressed genes in tumor were analyzed via R software. Interaction network of differently expressed miRNAs and differently expressed mRNA was established by means of Cytoscape software. CircCEP128 and miR‐145‐5p expression levels were determined using qRT‐PCR. The expression of MAPK signaling‐related proteins MYD88, p38, ERK and JNK was examined by western blot. The relationship between circCEP128 and miR‐145‐5p was validated using RNA immunoprecipitation. The level of cell propagation and migration was determined by CCK8 and wound healing assay, 5‐bromo‐2′‐deoxyuridine assay and migration assay. Cell apoptosis rate and cell cycle were detected via flow cytometry. Tumor xenograft assay was implemented to investigate the function of circCEP128 in vivo. CircCEP128 and MYD88 were overexpressed in bladder cancer based on microarray analysis and miR‐145‐5p was a potential targeting factor in bladder cancer. CircCEP128 targeted miR‐145‐5p and miR‐145‐5p targeted MYD88. Expression of miR‐145‐5p was decreased in cancer samples. Knockdown of circCEP128 induced the inhibition of cell viability and mobility and cell cycle arrest. Overexpression of miR‐145‐5p or knockdown of circCEP128 promoted MAKP signaling pathway and related proteins expression. In addition, knockdown of circCEP128 suppressed the growth of bladder cancer tumor tissues in vivo. Overexpression of circCEP128 promoted bladder cancer progression through modulating miR‐145‐5p and MYD88 via MAKP signaling pathway.

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Section: Discussionmentioning

confidence: 94%

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Sun

Zhao

Chen

et al. 2019

Intl Journal of Cancer

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“…VEGFA is a member of the growth factor family and mediates angiogenesis . Several studies have also reported that VEGFA can activate bladder cancer progression .…”

Section: Resultsmentioning

confidence: 99%

Mutations in gliclazide‐associated genes may predict poor bladder cancer prognosis

Wen

Gong

et al. 2019

FEBS Open Bio

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In recent years, an increasing number of patients have had diabetes and cancer simultaneously; thus, it is crucial for physicians to select hypoglycemic drugs with the lowest risk of inducing cancer. Gliclazide is a widely used sulfonylurea hypoglycemic drug, but its cancer risk remains controversial. Here, we explored the primary targets of gliclazide and its associated genes by querying an available database to construct a biological network. By using DrugBank and STRING , we found two primary targets of gliclazide and 50 gliclazide‐associated genes, which were then enrolled for Kyoto Encyclopedia of Genes and Genomes ( KEGG ) enrichment analysis using WebGestalt. From this analysis, we obtained the top 15 KEGG pathways. Accurate analysis of these KEGG pathways revealed that two pathways, one linked to bladder cancer and the other linked to the phosphoinositide 3‐kinase– AKT signaling pathway, are functionally associated with gliclazide, and from these we identified four overlapping genes. Finally, genomic analysis using cB ioPortal showed that genomic alterations of these four overlapping genes predict poor prognosis for patients with bladder cancer. In conclusion, gliclazide should be used with caution as a hypoglycemic drug for diabetic patients with cancer, especially bladder cancer. In addition, this study provides a functional network analysis to flexibly explore drug interaction systems and estimate their safety.

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“…[35] circRNACCDC66 was shown to accelerate the proliferation and metastasis of colon cancer, [36] circLARP4 regulated LARP4 expression by competitively binding to miR-424-5p in gastric cancer, [37] and circLMO7 was involved in the regulation of myogenic differentiation in cattle. circRNAs have been shown to play important roles in various cancers.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1‐Mediated Transcriptional Regulation of the Humanin Polypeptide Family

et al. 2018

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AbstractcircNOL10 is a circular RNA expressed at low levels in lung cancer, though its functions in lung cancer remain unknown. Here, the function and molecular mechanism of circNOL10 in lung cancer development are investigated using in vitro and in vivo studies, and it is shown that circNOL10 significantly inhibits the development of lung cancer and that circNOL10 expression is co‐regulated by methylation of its parental gene Pre‐NOL10 and by splicing factor epithelial splicing regulatory protein 1 (ESRP1). circNOL10 promotes the expression of transcription factor sex comb on midleg‐like 1 (SCML1) by inhibiting transcription factor ubiquitination and thus also affects regulation of the humanin (HN) polypeptide family by SCML1. circNOL10 also affects mitochondrial function through regulating the humanin polypeptide family and affecting multiple signaling pathways, ultimately inhibiting cell proliferation and cell cycle progression, and promoting the apoptosis of lung cancer cells, thereby inhibiting lung cancer development. This study investigates the functions and molecular mechanisms of circNOL10 in the development of lung cancer and reveals its involvement in the transcriptional regulation of the HN polypeptide family by SCML1. The results also demonstrate the inhibitory effect of HN on lung cancer cells growth. These findings may identify novel targets for the molecular therapy of lung cancer.

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Circular RNA MYLK as a competing endogenous RNA promotes bladder cancer progression through modulating VEGFA/VEGFR2 signaling pathway

Cited by 396 publications

References 29 publications

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Mutations in gliclazide‐associated genes may predict poor bladder cancer prognosis

Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1‐Mediated Transcriptional Regulation of the Humanin Polypeptide Family

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