Circular RNA ZNF609 drives tumor progression by regulating the miR-138-5p/SIRT7 axis in melanoma

Abstract

Melanoma serves as a prevailing and lethal skin malignancy with high mortality and a growing number of patients globally. Circular RNAs (circRNAs), as a type of emerging cellular regulator, are involved in the modulation of melanoma. Nevertheless, the function of circZNF609 in melanoma development remains obscure. In this study, we were interested in the effect and the underlying mechanism of circZNF609 on DNA damage during melanoma progression. The circZNF609 depletion significantly suppressed melanoma cell invasion, migration, and proliferation, and stimulated apoptosis. Meanwhile, comet assays showed that the tail length and γH2AX levels were elevated by circZNF609 depletion. Mechanically, circZNF609 sponged miR-138-5p and miR-138-5p targeted SIRT7 in the melanoma cells. The SIRT7 overexpression and miR-138-5p inhibitor could reverse circZNF609 depletion-mediated DNA damage and malignant progression in melanoma cells. Functionally, CircZNF609 promoted cell growth of melanoma in the nude mice. Consequently, we conclude that circZNF609 suppresses DNA damage and potentially enhances melanoma progression at the experimental condition by modulating the miR-138-5p/SIRT7 axis. Our finding provides new insights into the mechanism by which circZNF609 modulates the development of melanoma. CircZNF609 and miR-138-5p may be utilized as therapeutic targets for melanoma.

Isoliquiritigenin regulates the circ_0002860/miR-431-5p/RAB9A axis to function as a tumor inhibitor in melanoma

“…Circ_0020710 was upregulated in melanoma and promoted tumor progression in vitro [ 23 ]. CircRNA_0016418 and circZNF609 accelerated the biological behaviors including migration, invasion and EMT in melanoma cells [ 24 , 25 ]. Herein, circ_0002860 level was shown to be significantly elevated in melanoma samples and cells.…”

Non-coding RNA transcripts, incredible modulators of cisplatin chemo-resistance in bladder cancer through operating a broad spectrum of cellular processes and signaling mechanism

“…CircZNF609 is the other ncRNA, recently established to be related to migration in line with cisplatin resistance. Like many other circRNAs, it can induce tumor expansion by sponging diverse miRNAs [ 219 ]. For instance, by sponging miR-1200, circZNF609 can serve as an oncogene because miR-1200 generally blocks the tumor development and increases cisplatin sensitivity in a group of cancers [ [219] , [220] , [221] ].…”

Sirtuins at the Crossroads between Mitochondrial Quality Control and Neurodegenerative Diseases: Structure, Regulation, Modifications, and Modulators

“…Besides, lncRNA AC007207.2 drove the upregulation of SIRT7 by sponging miRNA-1306-5p in osteosarcoma cells [ 138 ], while lincRNA p21 elevated SIRT7 levels by sponging miRNA-17-5p in vascular smooth muscle cells. SIRT7 expression was regulated by circRNA ZNF609 and circRNA PVT1 via miRNA-138-5p [ 139 ] and miRNA-125b/miRNA-200a sponging [ 140 ], respectively.…”