Circular RNAs are miRNA sponges and can be used as a new class of biomarker

Kulcheski, Franceli Rodrigues; Christoff, Ana Paula; Margis, Rogério

doi:10.1016/j.jbiotec.2016.09.011

Cited by 654 publications

(524 citation statements)

References 97 publications

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“…As reported, circRNAs could function as a miRNA sponge to regulate the gene expression [26, 27] and be involved in cancer processes [28-30]. To dissect the selected circRNAs’ potential functions, the circRNA and miRNA interactions were predicted by using Arraystar’s homemade miRNA target prediction software based on TargetScan and miRanda.…”

Section: Resultsmentioning

confidence: 99%

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

Zhao

Wang

et al. 2017

Cell Physiol Biochem

106

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Background/Aims: Lung adenocarcinoma, a form of non-small cell lung cancer with high lethality at an advanced stage, is becoming more common in women, non- or never-smokers, and even young adults. At present, there are still no effective early diagnosis methods for patients to be cured in a timely manner. Circular RNAs (circRNAs), which are stable and conserved non-coding RNA in mammalian cells, have been reported to be widely involved in the processes of cancer disease. However, it is still a puzzle as to which specific circRNAs are involved in the development of early-stage lung adenocarcinoma. Methods: Tumor samples and paired adjacent normal tissues from 4 patients with early-stage lung adenocarcinoma were selected to investigate the expression profile of circRNAs by using a high-throughput circRNA microarray. Bioinformatic analyses were conducted to screen those differentially expressed circRNAs. qRT-PCR and sequencing were performed to assure the microarray data. Results: A total of 357 circRNAs were dysregulated in the tumor samples, which suggests potential roles in lung cancer. qRT-PCR detection showed that five selected circRNAs were identical to the microarray data, and the potential circRNA-miRNA interactions were predicted. Conclusion: This work illustrates that clusters of circRNAs are aberrantly expressed in early-stage lung adenocarcinoma, which might offer potential targets for the early diagnosis of this disease and new genetic insights into lung cancer.

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Section: Resultsmentioning

confidence: 99%

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

Zhao

Wang

et al. 2017

Cell Physiol Biochem

106

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“…To date, several studies have proved that circRNAs may regulate gene expression by manipulating miRNAs through its miRNA sponges function [36], and circRNAs also compete with mRNAs for miRNA binding in the cytoplasm and interfere in gene regulation [8]. MiRNAs control a large set of biological processes through direct interaction with their target mRNA, so circRNA sponge activity will also affect these pathways [37,38]. Importantly, circRNAs have become the new markers for diseases including cancers [10,39].…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

Zhang¹,

Yan²,

Lei³

et al. 2018

Oncotarget

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ALV-J (subgroup J avian leucosis virus) is a kind of oncogenic exogenousretrovirus and diseases associated with ALV-J have caused severe reproduction problems in the poultry industry worldwide. However, the pathogenesis of ALV-Jinduced tumor formation is still unclear. In recent years, circRNAs are validated to be related to cancer, which prompts us to explore whether aberrant expression of circRNAs exists in ALV-J-infected tumor samples. In this study, a total of 2822 differentially expressed circRNAs were detected by RNA sequencing in the tumor livers of ALV-J-susceptible chickens (n = 3) and the normal liver samples of ALV-J-resistant chickens (n = 3), among which 2808 circRNAs in the tumor liver samples were detected as unregulated compared to those in the normal liver samples. As circRNAs are considered as miRNA sponge, we predicted top 5 miRNA targets for 24 selected upregulated circRNAs in the ALV-J-induced tumor livers. CircRNA-miRNA-mRNA regulatory axes were also constructed to provide clear interaction relationship among them. Furthermore, Gene Ontology and KEGG pathway analyses were performed for miRNA target genes, the result of which implied that metabolism plays a key role in circRNAs alterations in ALV-J-induced tumor livers. In summary, we identified that circRNAs are involved in ALV-J-induced tumor formation in chickens. However, the pathogenicity mechanism still needs to be further explored.

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“…CircRNAs were speculated to serve as miRNA sponges, which may play regulatory roles by competing with mRNAs for shared binding miRNAs [22]. Hence, various studies have used construction of a circRNAmiRNA-mRNA interaction network to explore the functions of circRNAs [23][24][25].…”

Section: Construction Of Decircrna-mirna-demrna Interaction Networkmentioning

confidence: 99%

The Differentially Expressed Circular RNAs in the Substantia Nigra and Corpus Striatum of Nrf2-Knockout Mice

Yang¹,

Zhang²,

Lin³

et al. 2018

Cell Physiol Biochem

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Background/Aims: The nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway plays a protective role in both acute neuronal damage and chronic neurodegeneration-related oxidative stress. Circular RNAs (circRNAs) are involved with various diseases in the central nervous system (CNS). This study aimed to identify the key circRNAs involved in Nrf2-neuroprotection against oxidative stress. Methods: The differentially expressed circRNAs (DEcircRNAs) in the substantia nigra and corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice were identified by microarray analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) was then used to validate the expression of selected DEcircRNAs in the substantia nigra and corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice. Based on our previous microarray analysis of the differentially expressed mRNAs (DEmRNAs) in the substantia nigra and corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice, the DEcircRNA-miRNA-DEmRNA interaction network was constructed. Functional annotation of DEmRNAs that shared the same binding miRNAs with DEcircRNAs was performed using gene ontology (GO) and pathway analyses. Results: A total of 65 and 150 significant DEcircRNAs were obtained in the substantia nigra and corpus striatum of Nrf2 (-/-) mice, respectively, and seventeen shared DEcircRNAs were found in both these two tissues. The qRT-PCR results were generally consistent with the microarray results. The DEcircRNA-miRNA-DEmRNA interaction network and pathway analysis indicated that mmu_circRNA_34132, mmu_circRNA_017077 and mmu-circRNA-015216 might be involved with Nrf2-mediated neuroprotection against oxidative stress. Mmu_circRNA_015216 and mmu_circRNA_017077 might play roles in the Nrf2-related transcriptional misregulation and Nrf2-mediated processes of rheumatoid arthritis, respectively. In addition to these two processes, mmu_circRNA_34132 may be a potential regulator of Nrf2-mediated protection for diabetes mellitus and Nrf2-mediated defence against ROS in hearts. Conclusion: In conclusion, our study identified the key DEcircRNAs in the substantia nigra and corpus striatum of Nrf2 (-/-) mice, which might provide new clues for further exploring the mechanism of Nrf2-mediated neuroprotection against oxidative stress and other Nrf2-mediated processes.

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Circular RNAs are miRNA sponges and can be used as a new class of biomarker

Cited by 654 publications

References 97 publications

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

The Differentially Expressed Circular RNAs in the Substantia Nigra and Corpus Striatum of Nrf2-Knockout Mice

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