Circulating and Fecal microRNAs as Biomarkers for Inflammatory Bowel Diseases

Schönauen, Katharina; Le, Nha; Arnim, Ulrike von; Schulz, Christian; Malfertheiner, Peter; Link, Alexander

doi:10.1093/ibd/izy046

Cited by 113 publications

(114 citation statements)

References 38 publications

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“…Subsequently, all samples were stored at −80 °C until further analysis. In similar fashion, similar amount of feces (≈20 µL) was homogenized in QIAzolLysis Reagent using TissueRuptor and cel‐miR‐39 was added . Subsequent steps were as described above.…”

Section: Methodsmentioning

confidence: 99%

“…In similar fashion, similar amount of feces (20 µL) was homogenized in QIAzolLysis Reagent using TissueRuptor and cel-miR-39 was added. [9] Subsequent steps were as described above. The miRNA extraction from ascites is similar to serum and is described in detail previously.…”

Section: Rna Isolation From Serum Feces and Ascitesmentioning

confidence: 99%

“…Certain miRNAs, for instance miR‐21, are frequently deregulated in GI tumors and are suggested as potential tumor biomarkers . We and others have shown that miRNAs can be easily detected in feces and differential expression of miRNAs may be useful for diagnosis of CRC and inflammatory bowel diseases …”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6] We and others have shown that miRNAs can be easily detected in feces and differential expression of miRNAs may be useful for diagnosis of CRC [7,8] and inflammatory bowel diseases. [9] It is believed that dietary behavior, which is strongly related to factors such as social life (childhood, family), culture, personal health etc., may have a great impact on human health status including personal risk for metabolic, malignant, and inflammatory diseases. [10,11] Recently, the working group of the International Agency for Research on Cancer (IARC) has classified processed meat as "carcinogenic to humans" (Group 1) and red meat was classified as "probably carcinogenic to humans" (Group 2A).…”

Section: Introductionmentioning

confidence: 99%

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Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

Link

Thon

Schanze

et al. 2018

Molecular Nutrition Food Res

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Scope Diet is amongst the most crucial factors contributing to the multistep process of carcinogenesis. The role of exogenous microRNAs (miRNAs) is still debatable. In this proof‐of‐principle work, the presence of miRNAs in a variety of foods, its stability to processing, and detectability in GI mucosa and feces are studied and the effect of short‐term diet on human‐ or plant‐derived miRNAs in feces and blood is examined. Methods and results Animal and plant miRNAs are detected in all foods irrespective of processing. Animal‐derived foods showed the highest miRNA level and the lowest is found in cheese and milk. The impact of the short‐term vegetarian or meat‐rich diet on blood and feces miRNA is evaluated in healthy subjects using qPCR and Affymetrix profiling. Diet is not associated with changes in ultraconserved miRNAs. However, a vegetarian diet is associated with an increase of miR‐168 in feces but not in blood. Overall, plant miR‐168 is detectable in normal GI mucosa and in colorectal cancer. Conclusions Food provides a great source of miRNAs and diet may be associated with changes in xenomiRs. Plant‐derived miR‐168 is ubiquitously present in feces, normal mucosa, and cancer. Further studies are needed to evaluate the functional interaction between diet‐derived miRNAs and GI tract.

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Section: Methodsmentioning

confidence: 99%

Section: Rna Isolation From Serum Feces and Ascitesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

Link

Thon

Schanze

et al. 2018

Molecular Nutrition Food Res

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“…Serum miRNAs may also be valuable in IBD classification. Schonauen et al reported serum concentrations of miR-16, miR-21, and miR-223 were higher in IBD patients than healthy controls, and levels were higher in CD compared with UC (121). It is noted that increased expressions of miR-21 were also observed in subsets of macrophages and T cells in mucosal tissue of UC patients compared with CD patients (136).…”

Section: Micrornasmentioning

confidence: 98%

Serum Biomarkers for Inflammatory Bowel Disease

et al. 2020

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Background: Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, is a chronic, inflammatory disorder of the gastrointestinal tract. As the novel therapeutic goal and biologicals are widely recognized, accurate assessment of disease and prediction of therapeutic response have become a crucial challenge in clinical practice. Also, because of the continuously rising incidence, convenient and economical methods of diagnosis and clinical assessment are urgently needed. Recently, serum biomarkers have made a great progress and become a focus in IBD study because they are non-invasive, convenient, and relatively inexpensive than are markers in biopsy tissue, stool, breath, and other body fluids. Aims: To review the available data on serological biomarkers for IBD. Methods: We searched PubMed using predefined key words on relevant literatures of serum biomarkers regarding diagnosis, evaluation of therapeutic efficacy, surveillance of disease activity, and assessment of prognosis for IBD. Results: We reviewed serological biomarkers that are well-established and widely used (e.g., C-reactive protein), newly discovered biomarkers (e.g., cytokines, antibodies, and non-coding RNAs), and also recently advancements in serological biomarkers (e.g., metabolomics and proteomics) that are used in different aspects of IBD management. Conclusions: With such a wealth of researches, to date, there are still no ideal serum biomarkers for IBD. Serum profiling and non-coding RNAs are just starting to blossom but reveal great promise for future clinical practice. Combining different biomarkers can be valuable in improving performance of disease evaluation.

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The miR‐223/nuclear factor I‐A axis regulates inflammation and cellular functions in intestinal tissues with necrotizing enterocolitis

Chan

Leung

et al. 2021

FEBS Open Bio

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We previously demonstrated that microRNA(miR)‐223 is overexpressed in intestinal tissue of infants with necrotizing enterocolitis (NEC). The objective of the current study was to identify the target gene of miR‐223 and to investigate the role of the miR‐223/nuclear factor I‐A (NFIA) axis in cellular functions that underpin the pathophysiology of NEC. The target gene of miR‐223 was identified by in silico target prediction bioinformatics, luciferase assay, and western blotting. We investigated downstream signals of miR‐223 and cellular functions by overexpressing the miRNA in Caco‐2 and FHs74 cells stimulated with lipopolysaccharide or lipoteichoic acid (LTA). NFIA was identified as a target gene of miR‐223. Overexpression of miR‐223 significantly induced MYOM1 and inhibited NFIA and RGN in Caco‐2 cells, while costimulation with LTA decreased expression of GNA11, MYLK, and PRKCZ. Expression levels of GNA11, MYLK, IL‐6, and IL‐8 were increased, and levels of NFIA and RGN were decreased in FHs74 cells. These potential downstream genes were significantly correlated with levels of miR‐223 or NFIA in primary NEC tissues. Overexpression of miR‐223 significantly increased apoptosis of Caco‐2 and FHs74 cells, while proliferation of FHs74 was inhibited. These results suggest that upon binding with NFIA, miR‐223 regulates functional effectors in pathways of apoptosis, cell proliferation, G protein signaling, inflammation, and smooth muscle contraction. The miR‐223/NFIA axis may play an important role in the pathophysiology of NEC by enhancing inflammation and tissue damage.

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Circulating and Fecal microRNAs as Biomarkers for Inflammatory Bowel Diseases

Cited by 113 publications

References 38 publications

Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

Food‐Derived Xeno‐microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof‐of‐Principle Study

Serum Biomarkers for Inflammatory Bowel Disease

The miR‐223/nuclear factor I‐A axis regulates inflammation and cellular functions in intestinal tissues with necrotizing enterocolitis

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