Circulating and synovial fluid hyaluronan levels

Abstract

Objective. To assess the effect of intraarticular (IA) corticosteroid on hyaluronan (HA) concentrations in synovial fluid (SF) and serum and the clearance of '311-labeled albumin from the joints of patients with rheumatoid arthritis (RA), osteoarthritis (OA), and ankylosing spondylitis (AS).Methods. SF and serum were collected before and 2 weeks and 2 months after IA steroid injection. The HA concentration was assessed using an enzyme-linked immunosorbent assay and '3'I-albumin clearance from joints was assessed using an external gamma counter.Results. In RA patients, HA concentrations in the SF were increased following IA steroids, while the serum concentrations were decreased. In OA patients, HA concentrations in SF tended to increase initially (decreasing thereafter), and were associated with increased HA concentrations in serum. There were less marked alterations in the AS patients. Albumin clearance rates were decreased significantly (2 weeks postinjection) only in the RA patients. Estimated HA flux revealed discrepancies between the HA concentration and the rate of flux in RA and AS patients. Conclusions. These findings suggest that IA steroid injection is associated with a restoration in the Supported by the Arthritis and Rheumatism Council of Great Britain.

Hyaluronan in joint cavitation

“…Indeed, soon after its isolation [Z] and chemical analysis [3], it was found that synovial fluid of normal joints contains high-Mr HA at particularly high concentrations [4]. Recent studies show that HA levels are decreased in rheumatoid joints, and that they are partly restored by intra-articular corticosteroid treatment, which concomitantly improves joint function [5,6]. This is consistent with the notion Abbreviations used: AER, apical ectodermalridge; ECM, extracellular matrix; ERM, ezrin, radixin and moesin; GAG, glycosaminoglycan; HA, hyaluronan; HABP, HA-binding protein; HAS-AP, HA-synthaseassociated protein; MAPWERK, mitogenn-activated protein kinase/extracellular-signal-related kinase; NO, nitric oxide; RHAMM, receptor for HA-mediated motility; UDPGD, UDP-glucose dehydrogenase.…”

Inflammatory biomarkers in sera of patients with intervertebral disc degeneration

“…( 16 , 24 , 25 ) Extracellular matrix degradation and turnover result in the release of HA and HA fragments into the systemic circulation. ( 26 ) Different from patients who underwent anti-inflammatory treatment, low levels of HA were found in the circulation. ( 23 , 26 )…”

“…( 26 ) Different from patients who underwent anti-inflammatory treatment, low levels of HA were found in the circulation. ( 23 , 26 )…”

“…( 16 , 24 , 25 ) A degradação e o turnover da MEC resultam na liberação de fragmentos de AH e AH na circulação sistêmica. ( 26 ) Diferentemente, em pacientes após tratamento inflamatório, níveis baixos de AH são encontrados na circulação. ( 23 , 26 )…”

Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint

“…Twelve of the 29 synovial fluid proteins with significant difference between groups are commonly found in plasma, and 10 of these were decreased in the high glucosinolate group. In the inflamed joint, changes in the permeability of the synovium have been described, whereby increases in protein and solute concentration within the joint cavity have been observed 31, 32 . Resolution of aberrant synovial permeability by the high glucosinolate diet may offer one possible explanation for a reduction in the number of plasma proteins observed in this group, though this remains to be confirmed.…”