Circulating antibodies against faecal bacteria assessed by immunomorphometry: combining quantitative immunofluorescence and image analysis

Apperloo-Renkema, H. Z.; Wilkinson, Michael H. F.; Waaij, D. van der

doi:10.1017/s0950268800050494

Cited by 11 publications

(13 citation statements)

References 9 publications

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“…An obvious extension to the system was to combine immunofluorescence and morphological information (3,14). By dividing up the bacteria according to their morphological principal component scores into some 100 categories, and computing the median titre within each category, a map of titre as a function of shape could be computed.…”

Section: Combined Morphometry and Immunofluorescencementioning

confidence: 99%

“…It allows measurement of many flora parameters without the need for culturing. Several applications have been developed in Groningen, initially just to perform automated counting and morphometric analysis (21)(22)(23), later adding fluorometric analysis as well, including immunofluorescence measurements (2,3,(12)(13)(14)(15) and more recently fluorescence in situ hybridization (FISH) (4). In this paper we will review the progress made in the microbiological image processing programme in Groningen.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Measurements of Intestinal Ecology by Digital Image Analysed Microscopy

Wilkinson¹,

Schut²

1998

Bioscience Microflora

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Section: Combined Morphometry and Immunofluorescencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Measurements of Intestinal Ecology by Digital Image Analysed Microscopy

Wilkinson¹,

Schut²

1998

Bioscience Microflora

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“…The slides were read by an image analysis system, which divided the bacteria into different groups (morphotypes) on the basis of their micromorphology ( Fig. 1) and measured the antibody titres of the IgA-, IgG-and IgM-isotype against these morphotypes as described previously [16]. In brief, faecal samples were thawed and 0 5 g per sample was suspended by vortexing in 4-5 ml demineralized water with 0-5 % Tween 80 (Merck, Darmstadt, FRG) and some glass beads.…”

Section: Indirect Immunofluorescence (Iif) Offaecal Samplesmentioning

confidence: 99%

Host-microflora interaction in systemic lupus erythematosus (SLE): circulating antibodies to the indigenous bacteria of the intestinal tract

Apperloo-Renkema¹,

Bootsma

Mulder³

et al. 1995

Epidemiol. Infect.

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SUMMARYExperimental data suggest a role for the microflora in the disease expression of systemic lupus erythematosus (SLE). In active SLE anti-ds-DNA antibodies are supposed to be pathogenic by forming immune complexes with DNA. Bacteria might induce the production of anti-ds-DNA antibodies. To explore the relation between the host and his microflora in SLE in comparison with healthy controls we studied the prevalence of systemic antibodies to faecal bacteria that were discriminated by their morphology by indirect immunofluorescence.IgM titres against their own faecal microflora were found to be lower both in active and inactive SLE when compared to healthy individuals. IgG-class antibacterial antibodies were increased in inactive SLE but decreased in active SLE compared to inactive SLE and healthy controls, although plasma levels of total IgG were almost doubled in active SLE. The lower IgG antibacterial antibody titres in active SLE might possibly result from sequestration of these IgG antibodies in immune complexes, indicating a possible role for antibacterial antibodies in exacerbations of SLE.

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“…Indeed these antibodies have been measured in healthy human individuals directed against a facultatively anaerobic E. coli strain that colonized their intestines after oral contamination [24]. Circulating antibodies directed against their own anaerobic faecal bacteria could also be measured in healthy human individuals, indicating that translocation of intestinal bacteria actually had happened [25].…”

Section: Introductionmentioning

confidence: 99%

Host–microflora interaction in Systemic Lupus Erythematosus (SLE): colonization resistance of the indigenous bacteria of the intestinal tract

et al. 1994

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SUMMARYExperimental data suggest a role for the microflora in Systemic Lupus Erythematosus (SLE). Anti-ds-DNA antibodies may be pathogenic in SLE by forming immune complexes with DNA. Foreign bacteria in the intestines could constitute the stimulus for anti-ds-DNA antibody production in SLE. Colonization Resistance (CR) is the defence capacity of the indigenous microflora against colonization of the intestines by foreign bacteria. A low CR implies increase of translocation of bacteria and a higher chance of subsequent, possibly DNA-crossreacting antibacterial antibody production.We measured CR by a comprehensive biotyping technique in healthy individuals and patients with inactive and active SLE. CR tended to be lower in active SLE patients than in healthy individuals (P = 009, Wilcoxon one sided, with correction for ties). This could indicate that in SLE more and different bacteria translocate across the gut wall due to a lower CR. Some of these may serve as polyconal B cell activators or as antigens cross-reacting with DNA.

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Circulating antibodies against faecal bacteria assessed by immunomorphometry: combining quantitative immunofluorescence and image analysis

Cited by 11 publications

References 9 publications

Quantitative Measurements of Intestinal Ecology by Digital Image Analysed Microscopy

Quantitative Measurements of Intestinal Ecology by Digital Image Analysed Microscopy

Host-microflora interaction in systemic lupus erythematosus (SLE): circulating antibodies to the indigenous bacteria of the intestinal tract

Host–microflora interaction in Systemic Lupus Erythematosus (SLE): colonization resistance of the indigenous bacteria of the intestinal tract

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