Circulating Antigen of Toxoplasma gondii in Patients with AIDS: Significance of Detection and Structural Properties

Hassl, Andreas; Aspöck, Horst; Flamm, H

doi:10.1016/s0176-6724(88)80167-6

Cited by 11 publications

(7 citation statements)

References 21 publications

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“…However, as there was no pattern that was characteristic of acute infection, 10 presence of these antigens was not used to diagnose reactivated toxoplasmosis. In our study, antibodies were detected against 6,20,22,23,25, and 36 kDa antigens in 15 of 19 patients with reactivated toxoplasmosis, an antigen recognition pattern similar to that seen in acute primary toxoplasmosis. Unlike a previous study, 10 our work included some sera collected before and during the period of illness.…”

Section: Discussionsupporting

confidence: 73%

“…In particular, sera from patient 1, collected before illness, had an antigen recognition pattern characteristic of past infection. Demonstration of bands representing 6,22,23,25, and 36 kDa antigens in sera collected five weeks later coincided with a significant change in dye test titre, detection of specific IgM, and a worsening clinical condition. A similar reversion was observed in sera collected from patient 3, and this also corresponded with clinical deterioration and an increase in dye test titre, but specific IgM was not detected.…”

Section: Discussionmentioning

confidence: 90%

“…The results of patient 2 indicate that some low molecular weight antigens might not be detected in all sera, and some might be detected in sera as a result of past infection. Therefore, detection of four or more of the most intensely stained bands (6,20,22,23,25, and 36 kDa) was considered to be a positive result. Detection of antibodies against the 28, 29, and 31 kDa antigens could not be included because these were common both in acute and past infection (table 1).…”

Section: Resultsmentioning

confidence: 99%

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Improved diagnosis of reactivated toxoplasmosis

Ashburn

Davidson

Joss

et al. 1998

Molecular Pathology

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

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Improved diagnosis of reactivated toxoplasmosis

Ashburn

Davidson

Joss

et al. 1998

Molecular Pathology

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“…Dannemann et al did a prospective study of antigenaemia in 11 serum samples from HIV-positive subjects and did not detect antigens in any patients, although two subjects had acute toxoplasma encephalitis [25]. Hassl et al studied the presence of circulating antigens in serum and CSF samples from patients with stage I11 and IV HIV infection and detected circulating antigens in 32 serum samples from 20 patients by ELISA; antigenaemia correlated well with serological results and clinical symptoms [26]. However, in that study, strict criteria were not used in the diagnosis of toxoplasma encephalitis.…”

Section: Pcr Discussionmentioning

confidence: 99%

Evaluation of different techniques in the diagnosis of Toxoplasma encephalitis

Rodríguez

Martínez

Martı́nez

et al. 1997

Journal of Medical Microbiology

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This study evaluated the detection of antibodies, circulating antigens and parasite DNA by polymerase chain reaction (PCR) in the diagnosis of toxoplasma encephalitis. The detection of antibody classes and IgG avidity were not useful diagnostically. The detection of circulating antigens by the ELISA system described was not sufficiently sensitive. The detection of DNA by PCR was the most useful test especially in untreated patients, with a sensitivity of 62% overall, 8lY0 in untreated patients and only 20% in treated patients. The use of non-isotopic probes makes the use of this technique feasible in routine diagnostic parasitology laboratories.

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“…Antibody titration is not the usual diagnostic procedure for the detection of Toxoplasma infection in AIDS patients. However, an alternative serological procedure, the detection of circulating antigens, is considered to be useful for serodiagnosis (Hassl et al 1988). Huskinson et al (1989) reported the detection of T. gondii antigens in urine samples of AIDS patients by ELISA.…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii Antigenuria in Patients with Acquired Immune Deficiency Syndrome

Fachado

Fonseca²,

Fonte³

et al. 1997

Mem. Inst. Oswaldo Cruz

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A longitudinal study was performed with sera and urine of patients with acquired immune deficiency syndrome (AIDS), taken before, during and after clinicallyToxoplasma gondii is recognized as an important pathogen during pregnancy and the perinatal period (Remington & Desmont 1983). It has recently gained additional interest as a cause of life threatening opportunistic diseases in immunocompromised individuals, including patients with acquired immune deficiency syndrome (AIDS) and transplant recipients (Postman et al. 1988). Toxoplasmosis is a major opportunistic parasitic disease in AIDS patients. Toxoplasmic encephalitis (TE) can only be diagnosed very late since the classical serological tests based on the detection of anti-T. gondii IgG and IgM are inefficient in these patients (Luft & Remington 1988). More sensitive diagnostic tests for the early diagnosis of TE are needed.The detection of antigens or parasites of T. gondii in different biological fluids is an accurate and rapid diagnosis. Recently, some researchers have described the use of T. gondii DNA sequence for the diagnosis of toxoplasmosis using the polymerase chain reaction (PCR) (Dupcuy-Camet et al. 1993). We have reported the use of coagglutination assay to detect T. gondii antigens in the urine of infected mice (Fachado et al. 1990) and also in the urine of AIDS patients (Fachado et al. 1992 We have developed the coagglutination technique using urine of AIDS patients with acute cerebral toxoplasmosis. In order to confirm the toxoplasmic origin of the antigens detected by CoAToxo, the immunoblot was employed. The results also were correlated with clinical symptoms of toxoplasmosis, response to specific chemotherapy and anatomopathologic exam if the patient died. MATERIALS AND METHODSProduction of the soluble antigenic extract -A soluble antigenic extract was prepared from the RH strain of T. gondii. The trophozoites were taken from peritoneal exudates of mice, three days after infection. The exudate was centrifuged at 650xg for 10 min in 2 ml of 0.1M phosphate buffer saline (PBS), pH 7.2-7.4 to eliminate host cell residues. The supernatant was discarded and the pellet resuspended with 2ml PBS and repeatedly passed through a 26 gauge needle in order to break the macrophages. It was again centrifuged at 160xg during 10 min at 4ºC and the supernatant centrifuged at 650xg during 10 min at 4ºC. The pellet was washed three times in 50 ml PBS. The suspension was then sonicated at 4ºC to obtain the parasites antigens, and centrifuged at 650xg during 2 hr at 4ºC . The protein concentration of the T. gondii antigens suspension was determined by the method of Lowry et al. (1951).Production of antiserum -The anti-Toxoplasma antiserum was prepared by dilution of 0.5 ml of the T. gondii antigens (1.3 mg/ml of protein) in the same volume of PBS. The diluted antigenic solu-

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Circulating Antigen of Toxoplasma gondii in Patients with AIDS: Significance of Detection and Structural Properties

Cited by 11 publications

References 21 publications

Improved diagnosis of reactivated toxoplasmosis

Improved diagnosis of reactivated toxoplasmosis

Evaluation of different techniques in the diagnosis of Toxoplasma encephalitis

Toxoplasma gondii Antigenuria in Patients with Acquired Immune Deficiency Syndrome

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