Circulating cell-free DNA is associated with cardiometabolic risk factors: The Health 2000 Survey

Jylhävä, Juulia; Lehtimäki, Terho; Jula, Antti; Moilanen, Leena; Kesäniemi, Y. Antero; Nieminen, Markku S.; Kähönen, Mika; Hurme, Mikko

doi:10.1016/j.atherosclerosis.2013.12.022

Cited by 53 publications

(58 citation statements)

References 24 publications

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“…A higher concentration of ccf‐DNA has been associated with different cancer types and the concentration of ccf‐DNA was found higher in cancer patients than the normal population [Leon et al, ; Papadopoulou et al, ]. In addition, ccf‐DNA has been associated with many non‐malignant diseases [Pathak et al, ] and with various cardiovascular risk factors [Jylhävä et al, ].…”

Section: Discussionmentioning

confidence: 99%

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

Çayır

Çoşkun

Çobanoğlu

2017

Environ and Mol Mutagen

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Pesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis-block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf-DNA) in the blood of pesticide-exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf-DNA levels using a Qubit fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide-exposed individuals were significantly higher than non-exposed individuals. Apart from MNL, BNMN and CBPI, a season-related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf-DNA level in the pesticide-exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf-DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf-DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf-DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161-169, 2018. © 2017 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

Çayır

Çoşkun

Çobanoğlu

2017

Environ and Mol Mutagen

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“…For example, elevated cf-DNA concentrations have been associated with the severity of a variety of chronic conditions including but not limited to cancer 2 , cardiovascular disease 3 , pre-eclampsia 4 , sepsis 5 , sleep apnea 6 and aging 7 . cf-DNA concentrations have also been shown to be related to the degree of injury after a traumatic events such as myocardial infarction 8 , strokes 9 , blunt trauma 10 and burn victims 8 .…”

Section: Introductionmentioning

confidence: 99%

Changes in cell free DNA during a college soccer season

Gentles¹,

Hornsby²,

Gray³

et al. 2015

Journal of Trainology

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Objectives: This study investigated chronic changes in cell free DNA (cf-DNA) throughout a collegiate soccer season. The relationship between cf-DNA, C-reactive protein (CRP), creatine kinase (CK), testosterone (T), cortisol (C), testosterone-cortisol ratio (T:C), body mass and body composition were also examined. Design: Longitudinal study design with repeated measures and group comparisons. Methods: Twenty three NCAA Division I male soccer players were divided into two groups. Starters were placed in Group 1 (G1) and non-starters were placed in Group 2 (G2). cf-DNA, CRP, CK, T, C, T:C, body mass and body composition were taken three times, corresponding to pre-season, approximately mid-season and immediately after the concluding the season. Results: In G1, cf-DNA, CRP, CK, cf-DNA %∆, CRP %∆ and, CK %∆ were all statistically higher at T2 and T3 than T1. In G2, CRP %∆ was statistically higher at T2 than T1. In G2, cf-DNA %∆, CRP %∆ and CK %∆ were higher at T2 and T3 than T1. Conclusions: This suggests that cf-DNA may be a useful marker that can reflect accumulated soccer training and competitive stressors.(Journal of Trainology 2015;4:25-31)

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“…Therefore, this result that cfDNAs are related with SBP in HD patients and diabetic HD patients suggested that cfDNA elevation can be influenced by hypertension like other diseases mentioned before. Recently, the presence of free nucleic acids in the peripheral circulation, referred to as cfDNA, has been proposed as a novel biomarker of CV risk [19] . Amanda et al [20] demonstrated that cfDNA levels are not influenced by renal impairment but do reflect endothelial dysfunction in patients with chronic kidney disease (CKD).…”

Section: Discussionmentioning

confidence: 99%

Effect of blood pressure and glycemic control on the plasma cell-free DNA in hemodialysis patients

Jeong

Moon

Choi

et al. 2015

Kidney Research and Clinical Practice

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BackgroundThe plasma levels of cell-free DNA (cfDNA) are known to be elevated under inflammatory or apoptotic conditions. Increased cfDNA levels have been reported in hemodialysis (HD) patients. The aim of this study was to investigate the clinical significance of cfDNA in HD patients.MethodsA total of 95 patients on HD were enrolled. We measured their predialysis cfDNA levels using real-time EIF2C1 gene sequence amplification and analyzed its association with certain clinical parameters.ResultsThe mean plasma cfDNA level in the HD patients was 3,884 ± 407 GE/mL, and the mean plasma cfDNA level in the control group was 1,420 ± 121 GE/mL (P < 0.05). Diabetic patients showed higher plasma cfDNA levels compared with nondiabetic patients (P < 0.01). Patients with cardiovascular complications also showed higher plasma cfDNA levels compared with those without cardiovascular complication (P < 0.05). In univariable analysis, the cfDNA level was associated with 3-month mean systolic blood pressure (SBP), white blood cell, serum albumin, creatinine (Cr), normalized protein catabolic rate in HD patients. In diabetic patients, it was significantly correlated with SBP, hemoglobin A1c, and serum albumin. In multivariate analysis, SBP was the independent determinant for the cfDNA level. In diabetic patients, cfDNA level was independently associated with hemoglobin A1c and SBP.ConclusionsIn patients with HD, cfDNA is elevated in diabetic patients and patients with cardiovascular diseases. Uncontrolled hypertension and poor glycemic control are independent determinants for the elevated cfDNA. Our data suggest that cfDNA might be a marker of vascular injury rather than proinflammatory condition in HD patients.

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Circulating cell-free DNA is associated with cardiometabolic risk factors: The Health 2000 Survey

Cited by 53 publications

References 24 publications

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

Changes in cell free DNA during a college soccer season

Effect of blood pressure and glycemic control on the plasma cell-free DNA in hemodialysis patients

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