Circulating circular RNA hsa_circ_0023179 acts as a diagnostic biomarker for non-small-cell lung cancer detection

Abstract

Background: Lung cancer, the most prevalent cancer-related death worldwide, still lacks the means for early diagnosis. Because of the unique properties of the loop that make it stable in body uids, circular RNAs (circRNAs) as a biomarker becomes a possibility. This research purposed to explore whether hsa_circ_0023179 can be applied as a possible biomarker for the early diagnosis and prognosis of non-small cell lung cancer (NSCLC).Methods: hsa_circ_0023179 was screened by high-throughput sequencing of three pairs of NSCLC tissues and their surrounding tissues. Agarose gel electrophoresis (AGE), Sanger sequencing, exonuclease digestion assay, and actinomycin D were used to a rm the molecular properties of circRNA. Precision determination was performed by placement at room temperature and multiple freezethawing test for methodological evaluation. The expression of hsa_circ_0023179 in tissues, serum, and cells was determined by quantitative real-time polymerase chain reaction (qRT-PCR) to establish the receiver operating characteristic (ROC) curve to assess the diagnostic e cacy of hsa_circ_0023179.Results: hsa_circ_0023179 conforms to the basic properties of circRNA, and the detection method of hsa_circ_0023179 has good stability and repeatability. Its expression was connected to histological type, TNM stage, lymph node metastasis, and distal metastasis in NSCLC tissues, serum, and cells. Compared with traditional tumor markers with higher sensitivity and speci city. A combined diagnosis can signi cantly improve the diagnostic value. The decrease in postoperative expression level suggests some potential for dynamic monitoring.Conclusion: hsa_circ_0023179 might be a promising novel serum marker for the detection and prediction of NSCLC.

Role of circular RNAs in lung cancer

“…A F-circEA, a circRNA generated by EML4-ALK fusion gene, is another potential liquid biopsy biomarker as it specifically exists in the plasma of EML4-ALK-positive NSCLC patients and contributes to tumor development by promoting cell migration and invasion ( Tan et al, 2018 ). An investigation into NSCLC diagnostic potential of a circulating circular RNA hsa_circ_0023179 revealed that its higher expression was connected to histological type, TNM stage, lymph node metastasis, and distal metastasis in NSCLC tissue, serum and cells ( Zhang et al, 2023 ). Moreover, it showed higher sensitivity and specificity when compared to traditional tumor markers.…”

Exploring the Potential of Non-Coding RNAs as Liquid Biopsy Biomarkers for Lung Cancer Screening: A Literature Review

“…Hang D. et al [113] adopted RNA sequencing (RNA-seq) and qRT-PCR approaches to explore cancer-related circRNAs expression, showing that circFARSA was increased in cancerous tissues, and was more abundant in the plasma of LC patients than controls. Other three circRNAs were tested as potential biomarkers for LC early detection with liquid biopsy showing a good diagnostic accuracy: hsa_circ_0023179 [114], hsa_circ_0006423 [115] and circFOXP1 [116].…”