Circulating endothelial cells in coronary artery disease and acute coronary syndrome

Schmidt, David E.; Manca, Marco; Hoefer, Imo E.

doi:10.1016/j.tcm.2015.01.013

Cited by 46 publications

(51 citation statements)

References 79 publications

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“…Moreover, circulating endothelial microparticles, released after endothelial cell activation or apoptosis, have been associated with an increased risk of acute coronary syndrome and thrombus formation, but inversely associated with myocardial function (21). In the present work, endothelial circulating MPs stand as a witness of IR-induced endothelial dysfunction.…”

Section: Discussionmentioning

confidence: 57%

An Intravenous Bolus of Epa

Burban

Meyer

Olland

et al. 2016

Shock

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Section: Discussionmentioning

confidence: 57%

An Intravenous Bolus of Epa

Burban

Meyer

Olland

et al. 2016

Shock

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“…CECs have been microscopically described already decades ago and their identity was confirmed by specific staining with endothelium‐specific antibodies . The origin of CECs, their detection methods, and the association with cardiovascular diseases have been reviewed . Already during vascular damage, CECs are released into the bloodstream suggesting that their increase precede that of established tissue‐damage markers like troponins or creatine kinase.…”

Section: Introductionmentioning

confidence: 99%

Circulating endothelial cells as biomarker for cardiovascular diseases

Farinacci

Krahn

Dinh

et al. 2019

Research and Practice in Thrombosis and Haemostasis

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BackgroundEndothelial dysfunction is involved in several cardiovascular diseases. Elevated levels of circulating endothelial cells (CECs) and low levels of endothelial progenitor cells (EPCs) have been described in different cardiovascular conditions, suggesting their potential use as diagnostic biomarkers for endothelial dysfunction. Compared to typical peripheral blood leukocyte subsets, CECs and EPCs occur at very low frequency. The reliable identification and characterization of CECs and EPCs is a prerequisite for their clinical use, however, a validated method to this purpose is still missing but a key for rare cell events.ObjectivesTo establish a validated flow cytometric procedure in order to quantify CECs and EPCs in human whole blood.MethodsIn the establishment phase, the assay sensitivity, robustness, and the sample storage conditions were optimized as prerequisite for clinical use. In a second phase, CECs and EPCs were analyzed in heart failure with preserved (HFpEF) and reduced (HFrEF) ejection fraction, in arterial hypertension (aHT), and in diabetic nephropathy (DN) in comparison to age‐matched healthy controls.ResultsThe quantification procedure for CECs and EPCs showed high sensitivity and reproducibility. CEC values resulted significantly increased in patients with DN and HFpEF in comparison to healthy controls. CEC quantification showed a diagnostic sensitivity of 90% and a sensitivity of 68.0%, 70.4%, and 66.7% for DN, HFpEF, and aHT, respectively.ConclusionA robust and precise assay to quantify CECs and EPCs in pre‐clinical and clinical studies has been established. CEC counts resulted to be a good diagnostic biomarker for DN and HFpEF.

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“…When CEPCs become mature endothelial cells, they lose the CD133 antigen, and this alteration can be used to distinguish CECs from CEPCs. 7,[11][12][13][14] CEPCs are immature precursor cells with proliferative potential that derive from the bone marrow and blood vessel walls. [4][5][6][7][8][9] Many different types of mechanisms can cause endothelial cells to detach from injured blood vessel walls, including apoptosis activation, mechanical injury, an imbalance between proangiogenic and anti-angiogenic factors, weakening of the intercellular connections, endothelial structure injury caused by cytokines/proteases, and the influence of various types of drugs.…”

Section: Introductionmentioning

confidence: 99%