Circulating endothelial stem cells are not decreased in pulmonary emphysema or COPD

Caramori, Gaetano; Rigolin, Gian Matteo; Mazzoni, Federico; Leprotti, S; Campioni, Paolo; Papi, Alberto

doi:10.1136/thx.2009.121640

Cited by 15 publications

(8 citation statements)

References 13 publications

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“…Studies have shown conflicting results with regard to neurologic disorders [35, 36] and asthma/COPD [36, 37]. Some have found that EPC are not decreased in COPD [38], while other investigators have shown that EPC are even influenced by whether patients are currently in a COPD exacerbation versus stable disease [39]. Thus, it is unclear at this time how these various diseases affected circulating EPC numbers in these patients.…”

Section: Discussionmentioning

confidence: 99%

Circulating endothelial progenitor cells inversely associate with organ dysfunction in sepsis

et al. 2012

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Purpose Endothelial dysfunction is a primary contributor to sepsis-related organ dysfunction and death. In sepsis animal models, endothelial progenitor cells (EPC) have contributed to vascular repair. The role of endothelial progenitor cells as a biomarker for organ dysfunction is still unknown. We hypothesized that circulating numbers of endothelial progenitor cells would be associated with improved outcomes in sepsis. Methods Prospective, observational single-center cohort study in adult intensive care units at Grady Memorial Hospital, an affiliate of Emory University, from July 2007 through April 2009. Peripheral blood was obtained from 95 patients with sepsis, 37 intensive care unit controls, and 51 healthy controls, of whom only 86 patients with sepsis were used in the analysis because we were not able to obtain enough blood in 9 sepsis patients. Clinical data were obtained, and organ dysfunction was measured by Sepsis-Related Organ Failure Assessment (SOFA) score. Endothelial progenitor cells were assessed by a colony-forming unit (CFU) assay in which peripheral blood mononuclear cells were isolated using Ficoll density-gradient centrifugation and cultured in growth media. Results The patients with sepsis had significantly lower mean endothelial progenitor cell colony counts compared with intensive care unit controls (p = 0.035) and healthy controls (p = 0.0005). There was no difference in colony counts between ICU controls and healthy controls (p = 0.81). In the sepsis patients, EPC CFU numbers inversely associated with SOFA score, adjusting for mortality (r2 = 0.05, p = 0.04). Conclusion Increased circulating endothelial progenitor cells inversely correlate with organ dysfunction in sepsis patients.

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Section: Discussionmentioning

confidence: 99%

Circulating endothelial progenitor cells inversely associate with organ dysfunction in sepsis

et al. 2012

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“…This led some authors to a misclassify HPCs as EPCs in the past and resulted in conflicting interpretations regarding EPC levels and function in disease states. This is particularly true for COPD, in which levels of circulating EPCs have been reported as being either the same as controls (14) or significantly reduced (15–17). A second limitation regarding studies that have measured EPCs in COPD has been the use of spirometry as the sole means of classifying subjects.…”

Section: Introductionmentioning

confidence: 94%

Circulating Hematopoietic Progenitor Cells are Decreased in COPD

Janssen

Yunt²,

Muldrow

et al. 2013

COPD

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Rationale Bone marrow derived progenitor cells participate in the repair of injured vessels. The lungs of individuals with emphysema have reduced alveolar capillary density and increased endothelial apoptosis. We hypothesized that circulating levels of endothelial and hematopoietic progenitor cells would be reduced in this group of patients. Objectives The goal of this study was to measure circulating levels of endothelial progenitor cells (EPCs) and hematopoietic progenitor cells (HPCs) in subjects with COPD and to determine if progenitor levels correlated with disease severity and the presence of emphysema. Methods Peripheral blood mononuclear cells were isolated from 61 patients with COPD and 32 control subjects. Levels of EPCs (CD45dim CD34+ ) and HPCs (CD45+ CD34+ VEGF-R2+) were quantified using multi-parameter flow cytometry. Progenitor cell function was assessed using cell culture assays. All subjects were evaluated with spirometry and CT scanning. Measurements and Main Results HPC levels were reduced in subjects with COPD compared to controls, whereas circulating EPC levels were similar between the two groups. HPC levels correlated with severity of obstruction and were lowest in subjects with severe emphysema. These associations remained after correction for factors known to affect progenitor cell levels including age, smoking status, the use of statin medications and the presence of coronary artery disease. The ability of mononuclear cells to form endothelial cell colony forming units (EC-CFU) was also reduced in subjects with COPD. Conclusions HPC levels are reduced in subjects with COPD and correlate with emphysema phenotype and severity of obstruction. Reduction of HPCs may disrupt maintenance of the capillary endothelium, thereby contributing to the pathogenesis of COPD.

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“…Their modulation in chronic pulmonary diseases is controversial [18]. EPC might be reduced [19,20], or not [21], in chronic obstructive pulmonary disease (COPD) as well as in hypoxemic patients with severe restrictive lung diseases [19,22]. Experimental and clinical studies have examined the potential contribution of EPC to the pathogenesis of pulmonary hypertension whether idiopathic or secondary, but again, results have been so far inconsistent [16].…”

Section: Introductionmentioning

confidence: 96%

Imbalance of circulating endothelial cells and progenitors in idiopathic pulmonary fibrosis

et al. 2012

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Circulating endothelial stem cells are not decreased in pulmonary emphysema or COPD

Cited by 15 publications

References 13 publications

Circulating endothelial progenitor cells inversely associate with organ dysfunction in sepsis

Circulating endothelial progenitor cells inversely associate with organ dysfunction in sepsis

Circulating Hematopoietic Progenitor Cells are Decreased in COPD

Imbalance of circulating endothelial cells and progenitors in idiopathic pulmonary fibrosis

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