Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo

Dong, Li; Zhou, Ting; She, Qiuyun; Nie, Xiaoqi; Liu, Zhong; Pan, Ronghua; Wei, Yujia; Deng, Yunhua

doi:10.1016/j.jid.2022.05.1086

Cited by 11 publications

(7 citation statements)

References 48 publications

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“…For instance, circulating exosomal miR-493-3p was significantly increased in segmental vitiligo (SV) patients. MiR-493-3p-overexpressing keratinocyte induced melanocyte apoptosis and decreased melanin synthesis through inhibiting HNRNPU expression and increasing dopamine secretion (46). Circulating exosomes from vitiligo patients, containing miR-21-5p, inhibited melanin content, tyrosinase activity, and melanogenesis-related protein expression in melanocytes via inhibiting SATB1 expression (47).…”

Section: Exosomes In Vitiligo Pathogenesismentioning

confidence: 99%

Exosomes: The emerging mechanisms and potential clinical applications in dermatology

Yu,

Feng,

Zeng

et al. 2024

Int. J. Biol. Sci.

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Skin tissue, composed of epidermis, dermis, and subcutaneous tissue, is the largest organ of the human body. It serves as a protective barrier against pathogens and physical trauma and plays a crucial role in maintaining homeostasis. Skin diseases, such as psoriasis, dermatitis, and vitiligo, are prevalent and can seriously impact the quality of patient life. Exosomes are lipid bilayer vesicles derived from multiple cells with conserved biomarkers and are important mediators of intercellular communication. Exosomes from skin cells, blood, and stem cells, are the main types of exosomes that are involved in modulating the skin microenvironment. The dysregulation of exosome occurrence and transmission, as well as alterations in their cargoes, are crucial in the complex pathogenesis of inflammatory and autoimmune skin diseases. Therefore, exosomes are promising diagnostic and therapeutic targets for skin diseases. Importantly, exogenous exosomes, derived from skin cells or stem cells, play a role in improving the skin environment and repairing damaged tissues by carrying various specific active substances and involving a variety of pathways. In the domain of clinical practice, exosomes have garnered attention as diagnostic biomarkers and prospective therapeutic agents for skin diseases, including psoriasis and vitiligo. Furthermore, clinical investigations have substantiated the regenerative efficacy of stem cell-derived exosomes in skin repair. In this review, we mainly summarize the latest studies about the mechanisms and applications of exosomes in dermatology, including psoriasis, atopic dermatitis, vitiligo, systemic lupus erythematosus, systemic sclerosis, diabetic wound healing, hypertrophic scar and keloid, and skin aging. This will provide a novel perspective of exosomes in the diagnosis and treatment of dermatosis.

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Section: Exosomes In Vitiligo Pathogenesismentioning

confidence: 99%

Exosomes: The emerging mechanisms and potential clinical applications in dermatology

Yu,

Feng,

Zeng

et al. 2024

Int. J. Biol. Sci.

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“…1,25(OH) 2 D 3 was freshly diluted with 0.9% NaCl before each experiment. Primary cultures of normal human epidermal melanocytes (NHEMs) were isolated and cultured as our previous experiments described [18]. Activated CD8+ T cells (2 × 105/well) were cultured with NHEMs for 12 h in 96-well flat-bottom plates after being pretreated with 1,25(OH) 2 D 3 at concentrations of 0 nM, 10 nM and 100 nM for 72 h, respectively.…”

Section: Procedures 221 Cell Culture and Treatmentmentioning

confidence: 99%

1,25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function

Wei,

Wang,

Nie

et al. 2023

Nutrients

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Vitiligo is a common autoimmune skin disease caused by autoreactive CD8+ T cells. The diverse effects of 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃] on immune cell metabolism and proliferation have made it an interesting candidate as a supporting therapeutic option in various autoimmune diseases. This study aimed to elucidate the immunomodulatory effects of 1,25(OH)₂D₃ in vitiligo. Cross-sectional relationships between serum 1,25(OH)₂D₃ levels and disease characteristics were investigated in 327 patients with vitiligo. The immunomodulatory and therapeutic effects of 1,25(OH)₂D₃ were then investigated in vivo and in vitro, respectively. We found that 1,25(OH)₂D₃ deficiency was associated with hyperactivity of CD8+ T cells in the vitiligo cohort. In addition, 1,25(OH)₂D₃ suppressed glycolysis by activating the AMP-activated protein kinase (AMPK) signaling pathway, thereby inhibiting the proliferation, cytotoxicity and aberrant activation of CD8+ T cells. Finally, the in vivo administration of 1,25(OH)₂D₃ to melanocyte-associated vitiligo (MAV) mice reduced the infiltration and function of CD8+ T cells and promoted repigmentation. In conclusion, 1,25(OH)₂D₃ may serve as an essential biomarker of the progression and severity of vitiligo. The modulation of autoreactive CD8+ T cell function and glycolysis by 1,25(OH)₂D₃ may be a novel approach for treating vitiligo.

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“…Furthermore, they discovered that miR-200C, downregulated in these exosomes, could promote melanogenesis, potentially mediated by the inhibition of SOX1 to activate the Wnt pathway. Li et al (16) performed high-throughput sequencing and correlation analysis of circulating exosomal miRNAs from segmental vitiligo patients and healthy controls with disease progression and staging, screened and expanded the specimens to verify that miR-493-3p, which was highly expressed in circulating exosomes of the patients as well as keratinocytes of the lesions. They subsequently demonstrated in vitro the miR-493-3p-hnRNPU-COMT-DA axis on the initial damage of melanocytes.…”

Section: Exosomal Mirnas In Vitiligomentioning

confidence: 99%

Exosomal miRNAs in autoimmune skin diseases

Zhang,

Wei,

Wang

et al. 2023

Front. Immunol.

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Exosomes, bilaterally phospholipid-coated small vesicles, are produced and released by nearly all cells, which comprise diverse biological macromolecules, including proteins, DNA, RNA, and others, that participate in the regulation of their biological functions. An increasing number of studies have revealed that the contents of exosomes, particularly microRNA(miRNA), play a significant role in the pathogenesis of various diseases, including autoimmune skin diseases. MiRNA is a class of single-stranded non-coding RNA molecules that possess approximately 22 nucleotides in length with the capability of binding to the untranslated as well as coding regions of target mRNA to regulate gene expression precisely at the post-transcriptional level. Various exosomal miRNAs have been found to be significantly expressed in some autoimmune skin diseases and involved in the pathogenesis of conditions via regulating the secretion of crucial pathogenic cytokines and the direction of immune cell differentiation. Thus, exosomal miRNAs might be promising biomarkers for monitoring disease progression, relapse and reflection to treatment based on their functions and changes. This review summarized the current studies on exosomal miRNAs in several common autoimmune skin diseases, aiming to dissect the underlying mechanism from a new perspective, seek novel biomarkers for disease monitoring and lay the foundation for developing innovative target therapy in the future.

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Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo

Cited by 11 publications

References 48 publications

Exosomes: The emerging mechanisms and potential clinical applications in dermatology

Exosomes: The emerging mechanisms and potential clinical applications in dermatology

1,25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function

Exosomal miRNAs in autoimmune skin diseases

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