Circulating immune complexes of Hodgkin's disease contain an antigen that is present in Hodgkin and reed-sternberg cells

Abstract

Circulating immune complexes (CIC), isolated from the serum of a patient with Hodgkin's disease (HD) and from control serum (CS) of healthy adults, were used to generate heterologous antisera in rabbits. The antiserum directed against CIC from HD (AS-HD) and the antiserum directed against CIC from CS (AS-CS) were used to identify immunoglobulins, complement factors and alpha2-macroglobulin as immune complex components. After adsorbing both antisera with normal human sera, we found that the adsorbed AS-HD was immunoreactive with radio-labelled CIC from HD serum but not with radiolabelled CIC from CS. Sera of patients with different diseases and sera of healthy adults were assessed for the occurrence of this Hodgkin immune complex-associated antigen (HIC-Ag). The HIC-Ag was present in 37% (12/33) of sera from patients with HD, 8% (8/101) of sera from patients with nonmalignant diseases, and 0% (0/6) of sera from healthy adults. This antigen was equally distributed among HD patients with and without symptoms, but its occurrence correlated with an advanced clinical stage of the disease. Using the adsorbed AS-HD in the immunoperoxidase technique, we identified the HIC-Ag as a cytoplasmic antigen in Hodgkin and Reed-Sternberg cells; whereas, the adsorbed AS-CS did not reveal any staining. These data indicate the presence of an HIC-Ag in the sera of patients with HD and suggest that the adsorbed AS-HD might be useful for isolation and characterization of this antigen for future use as a tumour marker.

Molecular Definition of a Novel Human Galectin Which Is Immunogenic in Patients with Hodgkin's Disease

“…Aberrations of the humoral immunity include the presence of immune complexes in sera of patients with Hodgkin's disease. Previous investigations revealed that these immune complexes contain antigens that are present in the cytoplasm of H&RS cells (37). However, the nature of these antigens has remained undefined.…”

Characterization of a lung‐cancer‐associated auto‐antigen

“…1975). Elevated levels of immune complexes are also associated with several types of human cancers, including melanoma (Jerry et al, 1976), GI malignancy (Ristow et a/., 1979), Hodgkin's disease (Bepler et al, 1985), and breast (Krieger et al, 1983) and lung cancer (Garcia et al, 1987;Bukh eral., 1988;Lowe et al, 1981). Circulating immune complexes have been found, by a variety of techniques, in the sera of patients with cancer, and levels have tended generally to correlate with stage of disease in most series (Ruiz-Arguelles et a/., 1982;Theofilopoulos et al, 1977).…”