Circulating levels of C1q/TNF‐α‐related protein 6 (CTRP6) in polycystic ovary syndrome

Abstract

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting females of reproductive age. It has been associated with cardiometabolic disorders including diabetes mellitus and cardiovascular disorders, and increases the risk of developing fecundity pathologies including recurrent pregnancy loss (RPL) and infertility. C1q/tumor necrosis factor‐α‐related protein‐6 (CTRP6) is a novel adipokine involved in glucose and lipid metabolism, host inflammation, and organogenesis. In the present study, we aimed to determine the association of serum CTRP6 levels with some components of metabolic syndrome in PCOS patients (infertile PCOS [inf‐PCOS] and PCOS‐RPL). This case–control study included 120 PCOS patients (60 inf‐PCOS and 60 PCOS‐RPL) and 60 healthy controls. Serum high‐sensitivity C‐reactive protein (hs‐CRP) and homocysteine were measured using commercial kits, while adiponectin and CTRP6 levels were assessed using ELISA technique. Inf‐PCOS and PCOS‐RPL individuals had higher levels of serum CTRP6 than controls (546.15 ± 125.02 ng/ml and 534.04 ± 144.19 ng/ml vs. 440.16 ± 159.24 ng/ml; both p < .001). Moreover, serum adiponectin levels were significantly reduced, while fasting insulin, homeostasis model assessment of insulin resistance, free testosterone, and hs‐CRP levels were significantly elevated in PCOS group, when compared with controls. Furthermore, serum CTRP6 positively associated with body mass index in all subjects. It showed an inverse correlation with adiponectin in PCOS group and subgroups. However, it had a direct association with hs‐CRP in PCOS group and inf‐PCOS subgroup, but not PCOS‐RPL subgroup. These findings unravel a probable role of CTRP6 in PCOS pathogenesis, which poses a possibility to be a good diagnostic target. However, further investigation is needed.

Circulating levels of Meteorin-like protein in polycystic ovary syndrome: A case-control study

“…Subjects were selected from the Obstetrics and Gynecology Department of Ibn Sina Infertility Center, Tehran, Iran, and controls were recruited from those performing routine checkups in the laboratory at the same center, from May 2017 to Jan 2018. The subjects were previously involved in our studies on PCOS and adipokines, and some of their data have been utilized in a recent publication [29].…”

“…This case-control study included 60 PCOS-RPL, 60 infertile PCOS (PCOS-Inf) and 60 control aged between 20-40 years, as described previously (published data) [29]. The inclusion criterion included PCOS diagnosis according to the 2003 Rotterdam Criteria [30], which dictates that two out of the following three characteristics should be met, PCOM on ultrasound, clinical and/or biochemical hyperandrogenism, and oligo-or ano-vulation (oligo-amenorrhea), with differential diagnosis of conditions such as hyperprolactinemia, thyroid diseases, premature ovarian failure, congenital adrenal hyperplasia, Cushing's syndrome, and adrenal tumors.…”

“…Five milliliters of venous blood were collected from each subject after overnight fasting at the follicular phase of their menstrual cycle. FBG, lipid profile (serum triglyceride (TG), total cholesterol (TC), LDL-C, HDL-C), homocysteine, hs-CRP, fasting insulin, free testosterone, FSH, and LH were measured as previously described [29]. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the following equation: [(FBG (mg/dL)] × [fasting blood insulin (μU/mL)] / 405 [33].…”

“…Measuring adipokines. Serum levels of adiponectin were measured using ELISA technique, as previously described [29]. Commercial ELISA kits were utilized to measure serum levels of Metrnl protein (Aviscera Biosciences, U.S.A.).…”

Evaluation of Lipid Ratios and Triglyceride-Glucose (TyG) Index as Risk Markers of Insulin Resistance in Iranian Polycystic Ovary Syndrome (PCOS) Woman

“…Folliclestimulating hormone (FSH), luteinizing hormone (LH), free T4, and homocysteine levels were evaluated using ELISA kits (Pishtaz Teb, Iran), according to manufacturer's instructions. Details about the intraassay coe cients of variation (CV) for biochemical parameters have been previously reported [20].…”