Circulating lncRNA BC030099 Increases in Preeclampsia Patients

Sun, Yuhong; Hou, Yan; Lv, Nan; Liu, Qian; Lin, Nan; Zhao, Sheng; Chu, Xiaodan; Chen, Xuan; Cheng, Guobin; Li, Peiling

doi:10.1016/j.omtn.2019.01.011

Cited by 20 publications

(12 citation statements)

References 13 publications

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“…With the development of next-generation sequencing, cell-free noncoding RNAs are attracting much attention as promising molecular biomarkers [34]. miRNAs, long noncoding RNAs, and circular RNAs are the focus of many clinical studies aimed at detecting common pregnancy complications, such as gestational diabetes [35,36] and preeclampsia [37,38]. However, no studies have reported the use of piRNAs as potential prenatal biomarkers.…”

Section: Discussionmentioning

confidence: 99%

PIWI-interacting RNA sequencing profiles in maternal plasma-derived exosomes reveal novel non-invasive prenatal biomarkers for the early diagnosis of nonsyndromic cleft lip and palate

et al. 2021

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Background: Congenital malformations are common birth defects with high neonatal morbidity and mortality. It is essential to find simpler and more efficient biomarkers for early prenatal diagnosis. Therefore, we investigated PIWI-interacting RNAs (piRNAs) as potential prenatal biomarkers in plasma-derived exosomes from pregnant women carrying foetuses with congenital malformations. Methods: Small RNA sequencing was used to screen piRNA biomarkers in plasma-derived exosomes of five pregnant women carrying foetuses with nonsyndromic cleft lip and palate (nsCLP) and five women carrying normal foetuses. Differentially expressed piRNAs were verified in 270 pregnant women, including 111 paired women carrying foetuses with congenital malformations and normal foetuses (at 24 gestational weeks), 10 paired women carrying foetuses with nsCLP and normal foetuses (at 15À19 gestational weeks), and 28 women at different stages of normal pregnancy. piRNA biomarkers were also verified in placentas, umbilical cords, fetal medial calf muscles, and lip tissues of nsCLP and normal foetuses. Findings: We identified a biomarker panel of three pregnancy-associated exosomal piRNAs (hsa-piR-009228, hsa-piR-016659, and hsa-piR-020496) could distinguish nsCLP foetuses from normal foetuses. These three piRNAs had better diagnostic accuracy for nsCLP at the early gestational stage, at which time typical malformations were not detected upon prenatal ultrasound screening, and had diagnostic value for neural tube defects (NTDs) and congenital heart defects (CHDs). Interpretation: Our work revealed the potential clinical applications of piRNAs for predicting nsCLP, NTDs, and CHDs.

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Section: Discussionmentioning

confidence: 99%

PIWI-interacting RNA sequencing profiles in maternal plasma-derived exosomes reveal novel non-invasive prenatal biomarkers for the early diagnosis of nonsyndromic cleft lip and palate

et al. 2021

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“…Sun et al showed that levels of lncRNA BC030099 in whole blood had a good ability to discriminate preeclamptic from healthy pregnant women (AUC = 0.713). Furthermore, lncRNAs NR_026824.1, AK055151.1, NR_027457, and NR_024178 were highly upregulated in PE placentas but did not demonstrate good biomarker potential for PE (AUC < 0.6) [ 145 ]. Luo et al suggested that levels of three lncRNAs in serum AF085938, G36948, and AK002210 can also serve as potential diagnostic biomarkers in preeclampsia (AUC = 0.7673, 0.7956, and 0.7575, respectively) [ 146 ].…”

Section: Long Non-coding Rnasmentioning

confidence: 99%

Non-Coding RNAs in Preeclampsia—Molecular Mechanisms and Diagnostic Potential

Munjas

Sopić

Stefanović

et al. 2021

IJMS

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Preeclampsia (PE) is a leading cause of maternal and neonatal morbidity and mortality worldwide. Defects in trophoblast invasion, differentiation of extravillous trophoblasts and spiral artery remodeling are key factors in PE development. Currently there are no predictive biomarkers clinically available for PE. Recent technological advancements empowered transcriptome exploration and led to the discovery of numerous non-coding RNA species of which microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the most investigated. They are implicated in the regulation of numerous cellular functions, and as such are being extensively explored as potential biomarkers for various diseases. Altered expression of numerous lncRNAs and miRNAs in placenta has been related to pathophysiological processes that occur in preeclampsia. In the following text we offer summary of the latest knowledge of the molecular mechanism by which lnRNAs and miRNAs (focusing on the chromosome 19 miRNA cluster (C19MC)) contribute to pathophysiology of PE development and their potential utility as biomarkers of PE, with special focus on sample selection and techniques for the quantification of lncRNAs and miRNAs in maternal circulation.

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“…Luo et al screened lncRNA expression profiles in PE patients' placentas, suggesting abnormal lncRNA expression may be one of the causes of PE [8]. Based on this, Sun et al detected 5 known uterusrelated lncRNAs' expression in 48 PE patients and 24 non-PE healthy subjects and found lncRNA BC030099 expression was significantly facilitated, indicating elevated plasma level of lncRNA BC030099 is related to an accelerated risk of PE and can be used as the potential biomarker to predict the occurrence of PE [9], but whether it is involved in the disease progression of PE and its specific mechanism still need to be further explored.…”

Section: Introductionmentioning

confidence: 99%

The Gut Microbiota Dysbiosis in Preeclampsia Contributed to Trophoblast Cell Proliferation, Invasion, and Migration via lncRNA BC030099/NF-κB Pathway

Tang

Xiao

et al. 2022

Mediators of Inflammation

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Background. Preeclampsia (PE) is the main reason of maternal and perinatal morbidity and mortality. Gut microbiota imbalance in PE patients is accompanied by elevated serum lipopolysaccharide (LPS) levels, but whether it affects the occurrence and development of PE, the underlying mechanism is not clear. This paper intends to investigate the relationship between lncRNA BC030099, inflammation, and gut microbiota in PE. Methods. The feces of the patients were collected, and gut microbiota changes were assessed by 16S rRNA sequencing and pathway analysis by PICRUSt. Next, we examined LPS and lncRNA BC030099 levels in feces or placenta of PE patients. Then, we knocked down lncRNA BC030099 in HTR-8/SVneo cells and added the NF-κB pathway inhibitor JSH-23. CCK-8 and Transwell assays were performed to determine cell proliferation, migration, and invasion. Western blot was utilized to evaluate MMP2, MMP9, snail, and E-cadherin, p-IκBα, IκBα, and nuclear NF-κB p65 levels. IL-6, IL-1β, and TNF-α levels were examined by ELISA. Results. Gut microbiota was altered in PE patients, and microbial genes associated with LPS biosynthesis were significantly elevated in gut microbiota in the PE group. LPS level in feces and placenta of PE group was significantly elevated. lncRNA BC030099 level in placenta of PE group was also notably promoted. Knockdown of lncRNA BC030099 promoted HTR-8/SVneo cell proliferation, migration, and invasion. Knockdown of lncRNA BC030099 also elevated MMP2, MMP9, and snail levels and repressed E-cadherin level. In addition, lncRNA BC030099 affected NF-κB pathway. Furthermore, NF-κB inhibitor reversed HTR-8/SVneo cell proliferation, invasion, and migration induced by LPS. Conclusions. The gut microbiota dysbiosis in PE contributed to HTR-8/SVneo cell proliferation, invasion, and migration via lncRNA BC030099/NF-κB pathway.

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Circulating lncRNA BC030099 Increases in Preeclampsia Patients

Cited by 20 publications

References 13 publications

PIWI-interacting RNA sequencing profiles in maternal plasma-derived exosomes reveal novel non-invasive prenatal biomarkers for the early diagnosis of nonsyndromic cleft lip and palate

PIWI-interacting RNA sequencing profiles in maternal plasma-derived exosomes reveal novel non-invasive prenatal biomarkers for the early diagnosis of nonsyndromic cleft lip and palate

Non-Coding RNAs in Preeclampsia—Molecular Mechanisms and Diagnostic Potential

The Gut Microbiota Dysbiosis in Preeclampsia Contributed to Trophoblast Cell Proliferation, Invasion, and Migration via lncRNA BC030099/NF-κB Pathway

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