Circulating Micro-RNAs as Potential Blood-Based Markers for Early Stage Breast Cancer Detection

Schrauder, Michael G.; Strick, Reiner; Schulz‐Wendtland, Rüdiger; Strissel, Pamela L.; Kahmann, Laura; Loehberg, Christian R.; Lux, Michael P.; Jud, Sebastian M.; Hartmann, Arndt; Hein, Alexander; Bayer, Christian; Bani, M. R.; Richter, Swetlana; Adamietz, Boris; Wenkel, Evelyn; Rauh, Claudia; Beckmann, Matthias W.; Fasching, Peter A.

doi:10.1371/journal.pone.0029770

Cited by 227 publications

(159 citation statements)

References 79 publications

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“…More recently, the roles of miRNAs as tumor biomarkers have been investigated and developed (40). miRNA expression profiles could identify the tissue origin of tumors (41,42), classify the subtypes of renal cell carcinomas (42), and facilitate the early-stage detection of breast, gastric, or lung cancers (43)(44)(45).…”

Section: Mir-451 In Cancer Diagnosismentioning

confidence: 99%

The Potential Role of miR-451 in Cancer Diagnosis, Prognosis, and Therapy

Pan

Wang

2013

Molecular Cancer Therapeutics

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MicroRNAs (miRNA) are small noncoding RNAs that converge to maintain an intrinsic balance of various processes, including cell proliferation, differentiation, and apoptosis. Recent research efforts have been devoted to translating these basic discoveries into applications that could improve the early diagnosis and therapeutic outcome of patients with cancer. Early studies have shown that miRNA-451 (miR-451) is widely dysregulated in human cancers and plays a critical role in tumorigenesis and tumor progression. In this review, we summarize the potential use of miR-451 for cancer diagnosis, prognosis, and treatment. In addition, we discuss the possible mechanisms of miR-451 dysregulation and future challenges in development of miR-451 as a noninvasive biomarker and a potential therapeutic target in human cancers.

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Section: Mir-451 In Cancer Diagnosismentioning

confidence: 99%

The Potential Role of miR-451 in Cancer Diagnosis, Prognosis, and Therapy

Pan

Wang

2013

Molecular Cancer Therapeutics

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“…Although numerous studies have focused on improving the treatment of OS in order to enhance patient prognosis, early detection of cancer remains highly recommended for the improvement of survival rates (6).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

Li¹,

Zhang²,

Liu³

et al. 2015

Oncology Letters

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Abstract. MicroRNAs (miRNAs) are a family of small non-protein coding RNAs, which regulate the expression of a wide variety of genes at the post-transcriptional level to control numerous biological and pathological processes. Various circulating miRNAs have been identified as potential diagnostic and prognostic biomarkers in multiple types of cancer and disease. The aim of the present study was to identify potential miRNA biomarkers for the early diagnosis and relapse prediction of osteosarcoma (OS). miRNA profiling was performed on serum from patients with osteosarcoma and healthy controls. All putative miRNAs were verified by reverse transcription-quantitative polymerase chain reaction analysis of 20 pre-therapeutic OS patients and 20 healthy individuals. The expression of miR-106a-5p, miR16-5p, miR-20a-5p, miR-425-5p, miR451a, miR-25-3p and miR139-5p was demonstrated to be downregulated in the serum of OS patients when compared with that of the healthy controls. Receiver-operating characteristic curve analyses indicated that these 7 miRNAs may be used as diagnostic biomarkers with the ability to discriminate between the healthy cohort and patients with OS. These results provide novel insights into the use of miRNAs in early blood screening for OS.

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“…What's more, miR-202, which were known to be involved in self-renewal and tumorigenicity of breast cancer cells, might be a new blood-based biomarkers for early stage breast cancer detection (Schrauder et al 2012). In order to gain insights into the function of these miRNAs, bioinformatics analysis (including prediction of The upper chart composes the GOs targeted by up-regulated miRNAs, and the lower chart composes the GOs targeted by down-regulated miRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…These three miRNAs have been described as crucial regulators in many biological processes and vascular diseases (Martin et al 2007;Chen and Hu 2012;Schrauder et al 2012) and thus they might also play important roles in CVI condition. As expected, the relative fold changes in miRNAs expression as determined by real-time RT-PCR were in agreement with microarrays results, as shown in Fig.…”

Section: Validation Of the Microarray Data By Real-time Rt-pcrmentioning

confidence: 99%

MicroRNA Profiling in Great Saphenous Vein Tissues of Patients with Chronic Venous Insufficiency

Cui

Guang

Huang

et al. 2012

Tohoku J. Exp. Med.

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Chronic venous insufficiency (CVI) is a common disease characterized by structural and functional abnormalities of the venous system. Until recently, the pathogenesis of CVI remains largely unknown. MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs emerged as post-transcriptional gene repressors and play essential roles in diverse pathological processes including vascular disease. However, their roles in CVI have not been elucidated. In this study, we employed oligonucleotide microarrays to perform a genome-wide miRNAs profiling in the great saphenous vein (GSV) tissues of patients with CVI. Our results revealed a total of 14 miRNAs that are expressed differentially in GSV tissues. Among them nine miRNAs were found significantly up-regulated, while five miRNAs were downregulated significantly. Real-time RT-PCR verified statistically consistent expression of three selected miRNAs (miR-34a, miR-155 and miR-202) with microarrays analysis. These three miRNAs, which were described as crucial regulators in many biological processes and vascular diseases, might also play important roles in CVI. Functional annotation of target genes of differentially expressed miRNAs via bioinformatics approaches revealed that these predicted targets were significantly enriched and involved in several key signaling pathways important for CVI, including mitogen-activated protein kinase pathways, pathways in cancer, apoptosis, and cell cycle, and p53 signaling pathways. In summary, miRNAs might involve in multiple signaling pathways contributing to the pathological processes of CVI. These data may provide fundamental insights into the molecular basis of CVI, which may aid in designing novel approaches for prevention and treatment of this complex disease.Keywords: bioinformatics; chronic venous insufficiency; great saphenous vein; microarray; microRNA Tohoku J. Exp. Med., 2012 Dec, 228 (4), 341-350. © 2012 Tohoku University Medical Press Chronic venous insufficiency (CVI) is a complex medical condition characterized by structural and functional abnormalities of the venous system, which is commonly seen in humans. CVI is manifested with a range of signs, including varicose veins, trophic skin changes and venous ulcers. It has a considerable socioeconomic impact in many countries due to its high prevalence, cost of investigations and management, deterioration of the quality of life and loss of working days (Rabe and Pannier 2010). To date, in spite of numerous studies on CVI, the etiology and pathogenesis of CVI remains largely unknown.MicroRNAs (miRNAs) are a family of highly conserved, endogenous small non-coding RNAs (19-24nt) that post-transcriptionally repress gene expression via degradation or translational inhibition of their target mRNAs. They play important roles in nearly all physiological and pathological processes, including cell differentiation, proliferation, apoptosis, cardiovascular disease, and human cancers (Bartel 2004). There is mounting evidence suggesting that miRNAs had distinct expression profiles an...

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Circulating Micro-RNAs as Potential Blood-Based Markers for Early Stage Breast Cancer Detection

Cited by 227 publications

References 79 publications

The Potential Role of miR-451 in Cancer Diagnosis, Prognosis, and Therapy

The Potential Role of miR-451 in Cancer Diagnosis, Prognosis, and Therapy

MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

MicroRNA Profiling in Great Saphenous Vein Tissues of Patients with Chronic Venous Insufficiency

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