Circulating micrometastases following oncological surgery

Denis, M. G.; Tessier, Marie-Hélène; Dréno, Brigitte; Lustenberger, P

doi:10.1016/s0140-6736(96)91402-6

Cited by 18 publications

(6 citation statements)

References 3 publications

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“…28 Using immunocytochemistry, circulating tumor cells are rarely found in peripheral blood samples from patients with no apparent signs of overt metastasis. 29,30 A large number of studies in minimal residual prostate cancer, the most extensively analyzed tumor entity using RT-PCR, have failed to demonstrate correlations between PSA RT-PCR results and pathological stage. Only one group supported the clinical relevance of PSA RT-PCR evaluating peripheral blood.…”

Section: Sources Of Rna and Rna Isolationmentioning

confidence: 99%

RT‐PCR‐Based Detection of Occult Disseminated Tumor Cells in Peripheral Blood and Bone Marrow of Patients with Solid Tumors: An Overview

Zippelius

Pantel

2000

Annals of the New York Academy of Sciences

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Despite recent progress in early detection and local curative therapy, patients with primary epithelial cancer quite frequently relapse with incurable metastasis. Early disseminated tumor cells that may be seminal for distant failure and are undetectable by current diagnostic methods have been identified by immunocytochemical techniques in bone marrow of cancer patients using monoclonal antibodies against cytokeratins. Recently, promising new molecular approaches, namely, reverse transcriptase-polymerase chain reaction (RT-PCR) assays, have been suggested as a potential technique for the detection of minimal residual tumor burden by targeting mRNA transcribed from epithelial genes in bone marrow, peripheral blood, or lymph nodes. Several studies using RT-PCR thus far indicate a highly sensitive and specific staging tool, although the prognostic value is still controversial. However, limitations may arise from ectopic expression of marker mRNA in hematopoietic cells and deficient expression in circulating tumor cells. The present review focuses on the relevant literature and demonstrates the range of current applications of RT-PCR-based assays for detecting disseminated tumor cells in peripheral blood and bone marrow of patients with solid tumors. We will both summarize technical evaluations of published molecular approaches and discuss the widely disparate results on PCR findings in clinical studies.

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Section: Sources Of Rna and Rna Isolationmentioning

confidence: 99%

RT‐PCR‐Based Detection of Occult Disseminated Tumor Cells in Peripheral Blood and Bone Marrow of Patients with Solid Tumors: An Overview

Zippelius

Pantel

2000

Annals of the New York Academy of Sciences

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“…However, a positive RT-PCR result postoperatively can be explained not only by surgical failure but also by intraoperative hematogenous dissemination of prostate cells. Although there is some evidence that hematogenous dissemination during radical prostatectomy occurs [25,49,69,70], the clinical significance remains uncertain [2,14,36,72]. A preliminary study by Fair et al [27] showed that the use of neoadjuvant hormone treatment before radical prostatectomy substantially reduced the postoperative PSM RT-PCR positivity and thereby the number of potentially "cured" patients.…”

Section: Discussionmentioning

confidence: 99%

Clinical usefulness of RT-PCR detection of hematogenous prostate cancer spread

1997

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Understaging is commonly associated with therapeutic failure of surgical intervention in apparently localized prostate cancers. Methods that specifically detect prostate cancer cells in the circulation may be able to identify metastatic cancers and thus aid in the selection of the most adequate therapy. The high sensitivity and specificity of the reverse transcriptase-polymerase chain reaction (RT-PCR) encouraged various groups to investigate the mRNA expression of prostate-specific markers in the peripheral blood of patients with prostate cancer. However, probably due to methodological differences, many contradictory results have been obtained with the markers studied so far: prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM). For this reason, clinical decisions should not be based yet on RT-PCR results. Future research and long-term follow-up on the patients may point out whether RT-PCR assays, following appropriate standardization, will have an additive value in prostate cancer staging and in prediction of tumor progression.

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“…It is recognized that during surgery cells are released into the circulation [94,95]. The blood is a temporary compartment for tumor cells, some cells survive in the circulation and some of these are able to form metastasis.…”

Section: Clinical Significance Of Circulating Tumor Cellsmentioning

confidence: 99%

Breast cancer circulating tumor cells

Carvalho

Laranjo²,

Abrantes³

et al. 2009

Oncol Rev

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Metastasization of breast cancer involves various mechanisms responsible for progression from invasive lesion to dissemination in distant organs. Regional lymph node metastasization was considered an initial step in this process, but it is now recognized that hematogenous dissemination is a deviation from lymphatic circulation. The detection of circulating tumor cells (CTC) is an aim in several oncology areas. For this purpose, several techniques have been used to detect CTC, including the use of antibodies and techniques with nucleic acids. This study reviews the published studies considering the detection of breast cancer CTC. There are focused the difficulties in identifying a CTC in a heterogeneous population, the handling of the sample, criteria of positivity, analytical techniques, and specific markers. There are systematized various specific markers of breast cancer cells also the problems with false positive results. Finally, we hypothesize clinical applications either as a prognostic marker or as a therapeutic response monitor.

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Circulating micrometastases following oncological surgery

Cited by 18 publications

References 3 publications

RT‐PCR‐Based Detection of Occult Disseminated Tumor Cells in Peripheral Blood and Bone Marrow of Patients with Solid Tumors: An Overview

RT‐PCR‐Based Detection of Occult Disseminated Tumor Cells in Peripheral Blood and Bone Marrow of Patients with Solid Tumors: An Overview

Clinical usefulness of RT-PCR detection of hematogenous prostate cancer spread

Breast cancer circulating tumor cells

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