Circulating Microparticles

Boulanger, Chantal M.; Amabile, Nicolas; Tedgui, Alain

doi:10.1161/01.hyp.0000231507.00962.b5

Cited by 331 publications

(155 citation statements)

References 98 publications

(62 reference statements)

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“…[23][24][25] Detections of circulating EMPs and baPWV are interesting and valuable tools to appreciate the ongoing and progression of hypertensive patients as well. Hypertension is accompanied by the reduced arterial elasticity and impaired endothelial function, which synergistically contribute to the pathogenesis of vascular disease.…”

Section: Discussionmentioning

confidence: 99%

Elevated circulating endothelial microparticles and brachial–ankle pulse wave velocity in well-controlled hypertensive patients

Wang

et al. 2008

J Hum Hypertens

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Vascular dysfunction in hypertensive condition is characterized by impaired endothelial function and reduced artery elasticity. Circulating endothelial microparticles (EMPs) and brachial-ankle pulse wave velocity (baPWV) are novel evaluation parameters for vascular function. However, their changes in patients with well-controlled blood pressure (BP) have not been fully acquired. To address this issue, circulating EMPs, defined as CD31 þ /CD42À MPs and baPWV were detected in 30 healthy subjects, 30 uncontrolled hypertensive (UCHT) patients and 23 well-controlled hypertensive (WCHT) patients. UCHT patients displayed elevated baPWV (Po0.01) and circulating EMPs (Po0.01) compared with healthy subjects. In WCHT patients, vascular damage represented by these two parameters constantly existed (Po0.01). Values of circulating EMPs were positively related to baPWV (Po0.01). Multivariate regression defined circulating EMPs as an independent contributor to the increase of baPWV value (Po0.05). Our study indicated that though BP was controlled, impaired endothelial function and arterial elasticity continued. The optimal therapy for patients with hypertension should include not only lowering BP but also improvement of vascular injury in parallel.

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Section: Discussionmentioning

confidence: 99%

Elevated circulating endothelial microparticles and brachial–ankle pulse wave velocity in well-controlled hypertensive patients

Wang

et al. 2008

J Hum Hypertens

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“…They are formed during apoptosis and range in size from 0.1 to 1.0 m. Their outer leaflets contain phosphatidylserine, which may be labeled with the phospholipid probe annexin V. Microparticles circulate in plasma and have recently emerged as potential inflammatory markers in cardiovascular disease (1).…”

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confidence: 99%

“…It can manifest as stable angina or as acute coronary syndrome (ACS). 1 The latter is a broad term describing a group of clinical symptoms consistent with acute myocardial ischemia. Its clinical spectrum includes unstable angina, non-ST elevation myocardial infarction, and ST elevation myocardial infarction.…”

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confidence: 99%

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Using Antibody Arrays to Detect Microparticles from Acute Coronary Syndrome Patients Based on Cluster of Differentiation (CD) Antigen Expression

Lal

Brown

Nguyen

et al. 2009

Molecular & Cellular Proteomics

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Microparticles circulate in plasma and have recently emerged as potential inflammatory markers in cardiovascular disease. They are fragments of cell membranes that express cluster of differentiation (CD) antigens and are present at elevated levels in patients with acute coronary syndrome. We have developed a novel method for the rapid detection of microparticles in plasma using a fluorescence-based antibody array system. Isolated microparticles are captured on anti-CD antibody spots immobilized on a nitrocellulose membrane. These CD antibodies are directed against extracellular epitopes, whereas the intracellular exposed surface of the microparticles is labeled with a fluorescent anti-annexin antibody. The array is then scanned and quantified. A pilot study was undertaken to compare microparticle CD antigen expression in acute coronary syndrome and healthy subjects. Ten CD antigens (44, 45, 54, 62E, 79, 102, 117, 130, 138, and 154) had significantly increased expression in the disease group relative to the healthy controls. These results were then verified using flow cytometry and scanning electron microscopy. Although we have focused our analysis on changes in microparticle CD antigen expression, this technique is amenable to analyzing other surface markers. Microparticles can be derived from a wide variety of cell types, so selection of the primary antibody can be tailored to the cell origin that is to be investigated. Molecular & Cellular Proteomics 8: 799 -804, 2009.

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“…31 The data of the literature shown that EMPs inversely correlate with the IIEF score in both diabetic and nondiabetic patients and multivariate analysis in the study of Boulanger, corrected for confounding factors, demonstrate that EMPs are the only independent predictor of the IIEF score. 30 In addition, ED patients with or without diabetes mellitus have significantly higher EMPs (CD62 pos ) than non-diabetic men without ED. The EMPs (CD62 pos )/EMPs (CD31 pos ) ratio, an index of endothelial activation (high ratio) or apoptosis (low ratio), is lowest in ED diabetic patients, therefore, these findings suggest that the EMP increase in ED diabetic patients is consistent with an increased apoptotic activity.…”

Section: Discussionmentioning

confidence: 96%

Original evaluation of endothelial dysfunction in men with erectile dysfunction and metabolic syndrome

et al. 2012

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We have recently demonstrated the diagnostic value of a new immunophenotype of blood endothelial progenitor cells (EPCs ¼ CD45neg/CD34pos/CD144pos) and endothelial microparticles (EMPs ¼ CD45neg/CD144pos/AnnexinVpos) in patients with arterial erectile dysfunction (ED) according to severity of cavernous arterial insufficiency evaluated through penile Doppler. The aim of this study was to evaluate both EPCs and EMPs in patients with arterial ED and metabolic syndrome (MetS), comparing these patients with another group of patients without MetS and ED and a third group with MetS but without ED. For this study 50 patients with arterial ED and MetS were selected (age: 55.0±3.0 years; range: 47--60). A group of age-matched (age: 54.0 ± 2.0 years; range: 44--60) patients without arterial ED and MetS INTRODUCTIONWe have recently demonstrated the diagnostic value of a new immunophenotype of blood endothelial progenitor cells (EPCs ¼ CD45neg/CD34pos/CD144pos) and endothelial microparticles (EMPs ¼ CD45neg/CD144pos/AnnexinVpos) in patients with arterial erectile dysfunction (ED) according to severity of cavernous arterial insufficiency evaluated through penile Doppler, showing that serum concentrations of these immunophenotypes are significantly higher compared with patients with psychogenic ED and among patients with arterial ED, those with severe arterial insufficiency showed significantly higher serum concentrations of EPCs and EMPs. 1 On this basis, the present study was undertaken to evaluate the number of EPCs and EMPs in patients with metabolic syndrome (MetS) and ED of arterial origin, which is a very common category among patients with sexual dysfunction in clinical practice. This clinical model seemed to us suitable to gain more insight into the balance between bone marrow endothelial repair mechanism and endothelial dysfunction in the manifestation of the clinical symptoms. To accomplish this, 50 patients with arterial dysfunctional-based ED and MetS, established by the Adult Treatment Panel (ATP) III criteria (NCEP ATPIII, 2002), were selected and the number of circulating EPCs and EMPs was determined. Two different groups of patients were enrolled as control: (a) patients without arterial ED and MetS; and (b) patients with MetS but without ED.

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Circulating Microparticles

Cited by 331 publications

References 98 publications

Elevated circulating endothelial microparticles and brachial–ankle pulse wave velocity in well-controlled hypertensive patients

Elevated circulating endothelial microparticles and brachial–ankle pulse wave velocity in well-controlled hypertensive patients

Using Antibody Arrays to Detect Microparticles from Acute Coronary Syndrome Patients Based on Cluster of Differentiation (CD) Antigen Expression

Original evaluation of endothelial dysfunction in men with erectile dysfunction and metabolic syndrome

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