Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway

Bai, Ming; Zhang, Mingzi; Long, Fei; Yu, Nanze; Zeng, Ang; Zhao, Ruizhi

doi:10.3892/or.2017.5537

Cited by 26 publications

(24 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…36 Studies have also shown that FOXO3A, as a tumor-suppressor gene, could induce the expressions of pro-apoptotic genes. 37,38 Thus, LY294002 may reverse the effects of HAX-1 on cell proliferation and migration by regulating the phosphorylation of relevant subunits (p110 and p85) or the expressions of downstream regulatory proteins (FOXO3A) in PI3K/Akt. In mammals, FOXO3A is involved in the development and progression of tumors by regulating survivin, p27, p21, and vascular endothelial growth factor (VEGF).…”

Section: Discussionmentioning

confidence: 99%

Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt

et al. 2019

Cancer Biology & Therapy

View full text Add to dashboard Cite

The aim of this study was to investigate the effects and mechanisms of hematopoietic-substrate-1-associated protein X-1 (HAX-1) on liver cancer cells. Information on HAX-1 from liver cancer patients was analyzed by the Cancer Genome Atlas (TCGA) program. Cell migration and invasion abilities were respectively tested by scratch assay and transwell assay. Tube formation assay was applied to detect angiogenesis protein and mRNA was determined using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. We found that the median month survival of HAX-1 overexpressing liver cancer patients was shorter than that of HAX-1 normal liver cancer patients. HAX-1 was overexpressed in liver cancer tissues and cells, and HAX-1 overexpression promoted the liver cancer cells growth, migration, and invasion, whereas silencing HAX-1 produced the opposite results. Inhibition of Akt by LY294002 reversed the migration and invasion abilities of liver cancer cells, and inhibited the ability of cells growth and angiogenesis. Silencing PIK3CA enhanced the inhibitory effects of HAX-1 silencing on the viability, migration, and invasion of liver cancer cells. HAX-1 affected liver cancer cells metastasis and angiogenesis by affecting Akt phosphorylation and FOXO3A expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt

et al. 2019

Cancer Biology & Therapy

View full text Add to dashboard Cite

show abstract

“…Besides, miR-194 also exerted its anti-proliferative effect on osteosarcoma cells via CDH2 and IGF1R [19]. Furthermore, miR-194 exhibited proapoptotic effect on melanoma cells by modulating PI3K/AKT/FoxO3a and p53/p21 signaling pathway [9].…”

Section: Discussionmentioning

confidence: 97%

“…Zhang et al reported that overexpression of miR-194 can inhibit migration and invasion in glioma cells [8]. Moreover, the proliferation of melanoma cells was suppressed by miR-194 overexpression [9]. However, the specific role of miR-194 in NSCLC and further underlying mechanisms have yet not been unveiled.…”

Section: Introductionmentioning

confidence: 99%

Suppression of proliferation, migration and invasion in non-small cell lung cancer cells via profilin 2 inhibition by microRNA-194

Jin-jiao¹,

Gao²,

Zhang³

et al. 2018

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To investigate the anti-tumor effect of microRNA-194 (miR-194) and possible relationship with profilin 2. Methods: The expressions of miR-194 and profilin 2 were investigated via bioinformatic analysis and were further verified using quantitative real-time polymerase chain reaction (qRT-PCR) in both normal and non-small cell lung cancer (NSCLC) cells. Dual luciferase reporter assay, together with transfection, was applied to determine the effect of miR-194 and profilin 2 on proliferation, migration and invasion, and its underlying mechanisms. Results: Relatively low expression of miR-194 and high expression of profilin 2 in cancerous lung tissue were found through bioinformatic analysis and further confirmed by qRT-PCR on normal and NSCLC cell lines. Profilin 2 was the direct target of miR-194. Also, miR-194 overexpression inhibited the proliferation, migration and invasion of NSCLC cells, and this suppressive effect was partially reversed by transfection of pcDNA3.1-PFN2 plasmid. Conclusion: The results show that miR-194 plays a tumor suppressor role in NSCLC development. It inhibits the proliferation, migration and invasion of NSCLC cells via profilin 2 suppression. Interventions targeting the miR-194/profilin 2 axis may therefore serve as a clinical strategy for the prevention and treatment of NSCLC metastases.

show abstract

“…The incidence continues to increase in the majority of the population [1][2][3]. It often shows a poor response to radiotherapy and chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

A phospholipase A2 of Daboia siamensis venom suppressed expression of genes and proteins in MAPK pathway

Khunsap¹,

Khow²,

Suntrarachun³

et al. 2020

ScienceAsia

View full text Add to dashboard Cite

High mortality rate of Melanoma is due to poor response to radiotherapy and chemotherapy. A phospholipase A 2 (PLA 2) of Daboia siamensis venom has been reported to inhibit melanoma cells. The aim of this study is to evaluate the effects of dssPLA 2 on gene and protein expression of MAPKs (Mitrogen-activated protien kinases) in melanoma cells. The mRNA and protein were determined by quantitative real-time PCR and western blotting to detect the expression levels of MEK1, MEK2, BRAF V600E and ERK1/2. Results indicated that 0.25 µg/ml of dssPLA 2 reduced the mRNA expression level of these genes in a time dependent manner. Interestingly, at 48 h, the mRNA expression level of all genes was extremely decreased compared with untreated cells in the same condition. Whereas, the protein level of MEK1, MEK2, BRAF V600E and ERK1/2 were clearly reduced after dssPLA 2 incubation. Our results show that dssPLA 2 is a strong inhibitor to MAPK genes at 48 h.

show abstract

Circulating microRNA-194 regulates human melanoma cells via PI3K/AKT/FoxO3a and p53/p21 signaling pathway

Cited by 26 publications

References 37 publications

Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt

Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt

Suppression of proliferation, migration and invasion in non-small cell lung cancer cells via profilin 2 inhibition by microRNA-194

A phospholipase A2 of Daboia siamensis venom suppressed expression of genes and proteins in MAPK pathway

Contact Info

Product

Resources

About