Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma

Uotani, Koji; Fujiwara, Tomohiro; Yoshida, Aki; Iwata, Shintaro; Morita, Takuya; Kiyono, Masahiro; Yokoo, Suguru; Kunisada, Toshiyuki; Takeda, Ken; Hasei, Joe; Numoto, Kunihiko; Nezu, Yutaka; Yonemoto, Tsukasa; Ishii, Takeshi; Kawai, Akira; Ochiya, Takahiro; Ozaki, Toshifumi

doi:10.1038/s41598-017-12660-5

Cited by 56 publications

(49 citation statements)

References 43 publications

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“…7 Although considerable previous research on determining the usefulness of miRNA as biomarkers has focused on analysis of tissue specimens, other researchers have focused on circulating miRNA as a potential diagnostic and prognostic biomarker. [8][9][10] Recently, miR-1246 and miR-1290 were identified as tumor-initiating, cell-specific miRNAs in non-small cell lung cancer. 11,12 Moreover, serum miR-1246 and miR-1290 levels were correlated with the clinical response of patients with lung cancer who were receiving chemotherapies.…”

Section: Introductionmentioning

confidence: 99%

Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study

et al. 2019

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MicroRNAs are a class of small, endogenous, noncoding 18-to 24-nucleotide-long RNAs that can regulate multiple processes related to cancer progression. However, their clinical value in patients with oral squamous cell carcinoma has not yet been fully explored. Therefore, the aim of this study was to investigate the clinical significance of circulating microRNAs in oral squamous cell carcinoma patients. The expression levels of circulating miR-1246 and miR-1290 in healthy volunteers and oral squamous cell carcinoma patients were examined by quantitative real-time polymerase chain reaction. The expression levels of both microRNAs in the radioresistant oral squamous cell carcinoma cell line (SAS-R) and the parent cell line (SAS) and in the conditioned medium obtained from these cell lines were also examined by quantitative real-time polymerase chain reaction. In addition, the correlations between circulating microRNA status and various clinicopathological features in 55 oral squamous cell carcinoma patients with locally advanced oral squamous cell carcinoma who underwent surgery following 5-fluorouracil-based chemoradiotherapy were examined. The expression level of miR-1290 was significantly lower in the plasma of oral squamous cell carcinoma patients than in that of healthy volunteers (p \ 0.01). The expression levels of microRNAs in the conditioned medium and in the cells varied from cell to cell. In the clinicopathological analyses, the frequency of patients with low miR-1290 levels was significantly higher among cases with lower pathological differentiation and among those with a poor pathological response for preoperative chemoradiotherapy (p = 0.030 each). Furthermore, Cox regression analysis based on the 5-year overall survival and disease-free survival revealed that miR-1290 status was a significant prognostic factor for patients with oral squamous cell carcinoma (hazard ratio = 0.169, p = 0.008, and hazard ratio = 0.186, p = 0.008, respectively). Circulating miR-1290 status could be a valuable biomarker for predicting the clinical response to chemoradiotherapy as well as overall survival in patients with oral squamous cell carcinoma.

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Section: Introductionmentioning

confidence: 99%

Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study

et al. 2019

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“…Members of the miR-200 family and miR-9/miR-9* are associated with SS and myxoid liposarcomas, respectively. Microarray profiling analysis performed on nine pairs of serum samples obtained from SS patients and healthy individuals identified 49 serum miRNAs that were significantly upregulated in SS patients compared with controls, reaffirming the potential clinical significance of serum miR-92b-3p for identifying SS tumors [42].…”

Section: Discussionmentioning

confidence: 67%

Signatures of circulating microRNA in four sarcoma subtypes

Koseła-Paterczyk¹,

Paziewska²,

Kulecka³

et al. 2020

J. Cancer

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Background: Sarcomas are rare malignant tumors of mesenchymal origin. The discovery of circulating biomarkers with high diagnostic value could supplement diagnosis of this heterogenous group of tumors. The aim of this study was to identify the profiles of circulating miRNA (c-miRNAs) in four groups of common bone and soft tissue sarcomas. Methods: At the time of diagnosis, blood samples were collected from 86 patients: 36 with locally advanced/unresectable/metastatic gastrointestinal stromal tumor (GIST) who received first-line treatment with imatinib; 16 with locally advanced osteosarcoma (OS); 26 with locally advanced synovial sarcoma (SS); and eight with locally advanced Ewing sarcoma (ES). In addition, samples were collected from 30 healthy controls. C-miRNAs were isolated using a miRCURY RNA Isolation Kit, followed by preparation of cDNA libraries and sequencing on the Ion Proton platform. Results: Pair-wise comparisons identified 156 unique c-miRNAs (adjusted P-value < 0.05) showing significant dysregulation between controls and patients; of these, 24, 36, 42, and 99 differentiated controls from pretherapeutic OS, SS, ES, and GIST, respectively. Ten c-miRNAs were commonly altered in at least three sarcoma types. Receiver operating characteristic curves and area under the curve (ROC-AUC) analyses revealed that a four-miRNA diagnostic classifier was able to differentiate controls from ES, GIST, OS, and SS, with AUC-ROC values of 1, 0.97, 0.95, and 0.94, respectively. Conclusions: Aberrant miRNA expression signatures were identified in serum from patients with four different sarcoma subtypes. Differences in miRNA expression profiles between sarcoma patients and healthy volunteers suggest that miRNAs may play a role in sarcoma development.

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“…There are three predicted sites in the Dkk3 3′UTR (Fig. 8I); two studies report Dkk3 is a direct target of miR-25 for other cell types (Huo et al, 2016;Yoshida et al, 2018) and two studies reporting that the miR-92 targets Dkk3 in neural tissue (De Brouwer et al, 2012;Haug et al, 2011). miR-92 has the same seed sequence as miR-25 ( Fig.…”

Section: Single Cell Rna-seq Analysis Of Reprogrammed Mgmentioning

confidence: 99%

MicroRNAs miR-25, let-7 and miR-124 regulate the neurogenic potential of Müller glia in mice

2019

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Müller glial cells (MG) generate retinal progenitor (RPC)-like cells after injury in non-mammalian species, although this does not occur in the mammalian retina. Studies have profiled gene expression in these cells to define genes that may be relevant to their differences in neurogenic potential. However, less is known about differences in micro-RNA (miRNA) expression. In this study, we compared miRNAs from RPCs and MG to identify miRNAs more highly expressed in RPCs, and others more highly expressed in MG. To determine whether these miRNAs are relevant to the difference in neurogenic potential between these two cell types, we tested them in dissociated cultures of MG using either mimics or antagomiRs to increase or reduce expression, respectively. Among the miRNAs tested, miR-25 and miR-124 overexpression, or let-7 antagonism, induced Ascl1 expression and conversion of ∼40% of mature MG into a neuronal/ RPC phenotype. Our results suggest that the differences in miRNA expression between MG and RPCs contribute to their difference in neurogenic potential, and that manipulations in miRNAs provide a new tool with which to reprogram MG for retinal regeneration.

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Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma

Cited by 56 publications

References 43 publications

Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study

Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study

Signatures of circulating microRNA in four sarcoma subtypes

MicroRNAs miR-25, let-7 and miR-124 regulate the neurogenic potential of Müller glia in mice

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