Circulating microRNA-944 and its target gene EPHA7 as a potential biomarker for colorectal cancer

Shaker, Olfat; Ayeldeen, Ghada; Abdelhamid, Amr M.

doi:10.1080/13813455.2020.1762658

Cited by 15 publications

(7 citation statements)

References 29 publications

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“…MicroRNAs are short RNA sequences that regulate gene expression by destabilising mRNA and inhibiting mRNA translation 10 . Specific microRNAs have been recognised to play a role in the activation of HSCs through a variety of signalling pathways 11 , 12 . HSC activation involves many signalling pathways such as TGFβ/smads signalling and Wnt/β-catenin pathway 13 .…”

Section: Introductionmentioning

confidence: 99%

Dasatinib ameliorates thioacetamide-induced liver fibrosis: modulation of miR-378 and miR-17 and their linked Wnt/β-catenin and TGF-β/smads pathways

Zaafan

Abdelhamid

2021

Journal of Enzyme Inhibition and Medicinal Chemistry

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Hepatic stellate cells activation (HSCs) plays a crucial role in the pathogenesis of liver fibrosis. Specific microRNAs have been suggested to affect the activation of HSCs via various signalling pathways including TGF-β/smads and Wnt/β-catenin pathways. Dasatinib is a multitarget inhibitor of many tyrosine kinases has recently studied for its anti-fibrotic effects in a variety of fibrous diseases. This study investigated the role of modulation of miRNA-378 and miRNA-17 in the pathogenesis of liver fibrosis through altering Wnt/β-catenin and TGF-β/smads pathways and evaluated the beneficial effect of the tyrosine kinase inhibitor, dasatinib, in thioacetamide-induced liver fibrosis model in mice. Treatment with dasatinib down-regulated miRNA-17 expression, leading to the restoration of WiF-1 and smad-7 which cause the inhibition of both Wnt/β-catenin and TGF-β/smads signalling. In addition, it upregulated miRNA-378 leading to the decrease of Wnt-10 which contributes to the suppression of activated HSCs.

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Section: Introductionmentioning

confidence: 99%

Dasatinib ameliorates thioacetamide-induced liver fibrosis: modulation of miR-378 and miR-17 and their linked Wnt/β-catenin and TGF-β/smads pathways

Zaafan

Abdelhamid

2021

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Cancer detection now has a low sensitivity since many cancers are not detected early enough, delaying therapy until it is too late ( Fiala and Diamandis, 2018 ). MiRNA expression is typically dysregulated in cancer ( Shaker et al, 2020 ), resulting in a distinct expression profile that aids in early cancer detection. MiRNAs linked to tumor growth are overexpressed, while suppressors are under-expressed ( Shaker et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Implication of miR-122, miR-483, and miR-335 Expression Levels as Potential Signatures in HCV-Related Hepatocellular Carcinoma (HCC) in Egyptian Patients

Elfert

Salem

Abdelhamid

et al. 2022

Front. Mol. Biosci.

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Hepatocellular carcinoma (HCC) is the second most common cause of cancer-related deaths worldwide with chronic hepatitis C virus (HCV) infection as a major risk factor of HCC. Circulating microRNAs are deregulated in HCC and are candidate biomarkers. The aim of this study was to explore the expression profile of miRNA-122, miR-483, and miR-335 in the serum of HCV-related hepatocellular carcinoma (HCC). 90 HCV-related hepatocellular carcinoma (HCC) patients, 90 non-malignant HCV patients, and 60 healthy controls were included. Serum microRNAs were measured by a qRT-PCR custom array. The expression levels of miR-122 and miR-483 were upregulated in HCC patients, while the miR-335 expression level was downregulated versus controls and HCV groups. Receiver-operating characteristic (ROC) curve analysis was created to examine miRNAs. miR-483 presented the best diagnostic potential because it showed the highest diagnostic accuracy for distinguishing HCV-related HCC patients from controls (AUC = 0.98) with 100% sensitivity. Moreover, there was obvious prognostic power in distinguishing HCV from HCC (AUC = 0.95) with 88% sensitivity. In conclusion, studied microRNAs (miR-122, miR-483, and miR-335) could serve as potential non-invasive early diagnostic biomarkers for HCC, and we identified a panel of three serum microRNAs with high accuracy in HCC diagnosis. Additional studies are required to confirm this panel and test its prognostic significance.

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“…Interestingly, Eph receptors show both tumor promoter and suppressor roles in human cancers. Indeed, EphA7 has been shown to play both tumor-suppressive and oncogenic roles in colorectal cancer, prostate cancer, and lung cancer [31,[37][38][39][40][41][42]. Upregulation of EphA7 in gallbladder adenocarcinoma and glioblastoma has also been shown to be related to metastasis and poor survival [33,36].…”

Section: Discussionmentioning

confidence: 99%

Loss of EphA7 Expression in Basal Cell Carcinoma by Hypermethylation of CpG Islands in the Promoter Region

Liu

Xiao

et al. 2022

Analytical Cellular Pathology

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Basal cell carcinoma (BCC) is the most common malignancy worldwide, with increasing incidence. BCCs present low mortality but high morbidity, and its pathogenesis remains unclear. Eph receptors have been implicated in tumorigenesis. EphA7 plays a role as a tumor suppressor in certain cancers. We checked EphA7 expression levels and methylation status in a set of BCCs, benign skin diseases, and compound nevus tissue samples using immunohistochemistry. EphA7 protein was positively expressed in normal basal cells, benign skin diseases, and compound nevus cells, but lost in areas of BCC tissues. We detected hypermethylation in BCC tissue samples with reduced expression of EphA7. There is a significant relationship between the expression level of EphA7 receptor protein and the methylation status of CpG islands in the EphA7 promoter region ( P < 0.001 ). To our knowledge, this is the first study to report the EphA7 expression profile and hypermethylation of EphA7 in BCC. The role of the EphA7 gene and the status of hypermethylation in tumorigenesis and treatment of BCC warrant further investigation.

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Circulating microRNA-944 and its target gene EPHA7 as a potential biomarker for colorectal cancer

Cited by 15 publications

References 29 publications

Dasatinib ameliorates thioacetamide-induced liver fibrosis: modulation of miR-378 and miR-17 and their linked Wnt/β-catenin and TGF-β/smads pathways

Dasatinib ameliorates thioacetamide-induced liver fibrosis: modulation of miR-378 and miR-17 and their linked Wnt/β-catenin and TGF-β/smads pathways

Implication of miR-122, miR-483, and miR-335 Expression Levels as Potential Signatures in HCV-Related Hepatocellular Carcinoma (HCC) in Egyptian Patients

Loss of EphA7 Expression in Basal Cell Carcinoma by Hypermethylation of CpG Islands in the Promoter Region

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