Circulating microRNA: Myocardium-derived prenatal biomarker of ventricular septal defects

Abstract

Background: Recently, circulating microRNAs (miRNAs) from maternal blood and amniotic fluid have been used as biomarkers for ventricular septal defect (VSD) diagnosis. However, whether circulating miRNAs are associated with fetal myocardium remains unknown.Methods: Dimethadione (DMO) induced a VSD rat model. The miRNA expression profiles of the myocardium, amniotic fluid and maternal serum were analyzed. Differentially expressed microRNAs (DE-microRNAs) were verified by qRT–PCR. The target gene of miR-1-3p was confirmed by dual luciferase reporter assays. Expression of amniotic fluid-derived DE-microRNAs was verified in clinical samples.Results: MiRNAs were differentially expressed in VSD fetal rats and might be involved in cardiomyocyte differentiation and apoptosis. MiR-1-3p, miR-1b and miR-293-5p were downregulated in the myocardium and upregulated in amniotic fluid/maternal serum. The expression of amniotic fluid-derived DE-microRNAs (miR-1-3p, miR-206 and miR-184) was verified in clinical samples. Dual luciferase reporter assays confirmed that miR-1-3p directly targeted SLC8A1/NCX1.Conclusion: MiR-1-3p, miR-1b and miR-293-5p are downregulated in VSD myocardium and upregulated in circulation and may be released into circulation by cardiomyocytes. MiR-1-3p targets SLC8A1/NCX1 and participates in myocardial apoptosis. MiR-1-3p upregulation in circulation is a direct and powerful indicator of fetal VSD and is expected to serve as a prenatal VSD diagnostic marker.

Prediction of miRNA‑mRNA network regulating the migration ability of cytarabine‑resistant HL60 cells

“…The present study, to the best of the authors' knowledge, is the first to comprehensively compare the integrated miRNA-mRNA network between HL60 and R-HL60 cells, and reveal that 16 miRNA-mRNA pairs, including miR-1-3p/ CCL2 , miR-1-3p/ SOX9 , miR-1-3p/ SLC8A1 , miR-155-5p/ CCL2 , miR-155-5p/ ICAM1 , miR-1255b-5p/ ICAM1 , miR-200c-5p/ CXCL10 , miR-3609/ CXCL10 , miR-1285-3p/ SIPR2 , miR-1285-3p/ CXCL10 , miR-124-3p/ FGFR1 , miR-124-3p/ OVOL2 , miR-124-3p/ MITF , miR-146a-5p/ CARD10 , miR-497a-5p/ CARD10 , and miR-3150a-3p/ CARD10 , participate in the regulation of migration ability during the development of cytarabine resistance in HL60 cells ( Table III ). Among them, miR-1-3p targeting CCL2 ( 28 ); miR-1-3p targeting SOX9 ( 29 ); miR-1-3p targeting SLC8A1 ( 30 ); and miR-146-5p targeting CARD10 ( 31 ) have been confirmed by luciferase assay in previous studies. In addition, miR-1-3p ( 32 ), miR-1255-5p ( 33 ), miR-200c-5p ( 34 ), miR-155-5p ( 35 ), miR-1285-3p ( 36 ), miR-124-3p ( 37 ) and miR-146a-5p ( 38 , 39 ) have been also reported to regulate cell migratory behavior in several cell models.…”