Circulating microRNA profiles predict the severity of COVID-19 in hospitalized patients

Gonzalo‐Calvo, David de; Benítez, Iván D.; Pinilla, Lucía; Carratalá, Amara; Moncusí‐Moix, Anna; Gort‐Paniello, Clara; Molinero, Marta; González, Jessica; Torres, Gerard; Bernal, M. Eguia; Pico, Silvia; Almansa, Raquel; Jorge, Noelia; Ortega, Alicia; Bustamante-Munguira, Elena; Gómez, José Manuel Naranjo; González-Rivera, Milagros; Micheloud, Dariela; Ryan, Pablo; Martínez, Alberto; Tamayo, Luís; Aldecoa, César; Ferrer, Ricard; Ceccato, Adrián; Fernández-Barat, Laia; Motos, Anna; Riera, Jordi; Menéndez, Rosário; Garcia-Gasulla, Darío; Peñuelas, Óscar; Torres, Antoni; Bermejo-Martín, Jesús F.; Barbé, Ferrán

doi:10.1016/j.trsl.2021.05.004

Cited by 118 publications

(132 citation statements)

References 47 publications

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“…In agreement with Garg et al, who investigated circulating microRNAs in critically ill COVID-19 patients, we discovered dysregulated miRNAs involved in inflammatory processes such as miR-21-5p and 126-5p [ 44 ]. In line with de Gonzalo-Calvo et al, who investigated the circulating miRNA profile in hospitalized patients admitted to clinical wards without requiring critical care and patients admitted to the intensive care unit (ICU), we found an upregulation of miR-27a-3p, miR-27b-3p, and miR-148a-5p that distinguished between ICU and ward patients [ 43 ]. Furthermore, like Donyavi et al we looked at cellular miRNAs in the acute and post-acute phase of COVID-19, we found miR-29a-3p and miR-146a-3p upregulated in our severe group of patients [ 42 ].…”

Section: Discussionsupporting

confidence: 85%

“…However, although there is very little or no literature about these miRNAs, they should be kept in mind as possible markers for progressive COVID-19 disease. Other miRNAs that were significantly deregulated in our analysis, such as miR-21-5p, miR-27, 126-5p, miR-146, or miR-142 have already attracted attention in other COVID-19 studies [ 42 , 43 , 44 ]. In agreement with Garg et al, who investigated circulating microRNAs in critically ill COVID-19 patients, we discovered dysregulated miRNAs involved in inflammatory processes such as miR-21-5p and 126-5p [ 44 ].…”

Section: Discussionmentioning

confidence: 87%

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The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

Duecker

Adam

Wirtz

et al. 2021

IJMS

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A high incidence of thromboembolic events associated with high mortality has been reported in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infections with respiratory failure. The present study characterized post-transcriptional gene regulation by global microRNA (miRNA) expression in relation to activated coagulation and inflammation in 21 critically ill SARS-CoV-2 patients. The cohort consisted of patients with moderate respiratory failure (n = 11) and severe respiratory failure (n = 10) at an acute stage (day 0–3) and in the later course of the disease (>7 days). All patients needed supplemental oxygen and severe patients were defined by the requirement of positive pressure ventilation (intubation). Levels of D-dimers, activated partial thromboplastin time (aPTT), C-reactive protein (CRP), and interleukin (IL)-6 were significantly higher in patients with severe compared with moderate respiratory failure. Concurrently, next generation sequencing (NGS) analysis demonstrated increased dysregulation of miRNA expression with progression of disease severity connected to extreme downregulation of miR-320a, miR-320b and miR-320c. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed involvement in the Hippo signaling pathway, the transforming growth factor (TGF)-β signaling pathway and in the regulation of adherens junctions. The expression of all miR-320 family members was significantly correlated with CRP, IL-6, and D-dimer levels. In conclusion, our analysis underlines the importance of thromboembolic processes in patients with respiratory failure and emphasizes miRNA-320s as potential biomarkers for severe progressive SARS-CoV-2 infection.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 87%

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

Duecker

Adam

Wirtz

et al. 2021

IJMS

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“…The profile of circulating miRNAs in hospitalized COVID-19 patients and the evaluation of their potential as biomarkers have also been studied by Gonzalo-Calvo et al [235]. Plasma miRNA profiling related to disease severity and mortality in ICU patients was performed using RT-qPCR.…”

Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 99%

“…The levels of those miRNAs were correlated with the duration of stay in the ICU. Therefore, plasma miRNAs might be considered new tools to help clinicians predict early deterioration in life among patients [235].…”

Section: Sensitivity and Specificity Of Microrna Profiling In Covid-19 Patientsmentioning

confidence: 99%

Anti-SARS-CoV-2 Strategies and the Potential Role of miRNA in the Assessment of COVID-19 Morbidity, Recurrence, and Therapy

Narożna

Rubiś

2021

IJMS

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Recently, we have experienced a serious pandemic. Despite significant technological advances in molecular technologies, it is very challenging to slow down the infection spread. It appeared that due to globalization, SARS-CoV-2 spread easily and adapted to new environments or geographical or weather zones. Additionally, new variants are emerging that show different infection potential and clinical outcomes. On the other hand, we have some experience with other pandemics and some solutions in virus elimination that could be adapted. This is of high importance since, as the latest reports demonstrate, vaccine technology might not follow the new, mutated virus outbreaks. Thus, identification of novel strategies and markers or diagnostic methods is highly necessary. For this reason, we present some of the latest views on SARS-CoV-2/COVID-19 therapeutic strategies and raise a solution based on miRNA. We believe that in the face of the rapidly increasing global situation and based on analogical studies of other viruses, the possibility of using the biological potential of miRNA technology is very promising. It could be used as a promising diagnostic and prognostic factor, as well as a therapeutic target and tool.

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“…Recent research has suggested that a unique non-coding RNA signature can aid in identifying the likelihood of developing specific disease outcomes [2]. Alterations of miRNA levels in the blood have been described in multiple inflammatory and infectious diseases, including SARS-related coronaviruses [3][4][5][6][7][8][9]. MiRNAs are endogenous small non-coding RNAs, around 22 nucleotides, that bind specific messenger RNAs (mRNAs) through complementary base-pairing [10].…”

Section: Introductionmentioning

confidence: 99%

SnoRNAs and miRNAs Networks Underlying COVID-19 Disease Severity

et al. 2021

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There is a lack of predictive markers for early and rapid identification of disease progression in COVID-19 patients. Our study aims at identifying microRNAs (miRNAs)/small nucleolar RNAs (snoRNAs) as potential biomarkers of COVID-19 severity. Using differential expression analysis of microarray data (n = 29), we identified hsa-miR-1246, ACA40, hsa-miR-4532, hsa-miR-145-5p, and ACA18 as the top five differentially expressed transcripts in severe versus asymptomatic, and ACA40, hsa-miR-3609, ENSG00000212378 (SNORD78), hsa-miR-1231, hsa-miR-885-3p as the most significant five in severe versus mild cases. Moreover, we found that white blood cell (WBC) count, absolute neutrophil count (ANC), neutrophil (%), lymphocyte (%), red blood cell (RBC) count, hemoglobin, hematocrit, D-Dimer, and albumin are significantly correlated with the identified differentially expressed miRNAs and snoRNAs. We report a unique miRNA and snoRNA profile that is associated with a higher risk of severity in a cohort of SARS-CoV-2 infected patients. Altogether, we present a differential expression analysis of COVID-19-associated microRNA (miRNA)/small nucleolar RNA (snoRNA) signature, highlighting their importance in SARS-CoV-2 infection.

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Circulating microRNA profiles predict the severity of COVID-19 in hospitalized patients

Cited by 118 publications

References 47 publications

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

The MiR-320 Family Is Strongly Downregulated in Patients with COVID-19 Induced Severe Respiratory Failure

Anti-SARS-CoV-2 Strategies and the Potential Role of miRNA in the Assessment of COVID-19 Morbidity, Recurrence, and Therapy

SnoRNAs and miRNAs Networks Underlying COVID-19 Disease Severity

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