Circulating microRNA signatures in mice exposed to lipoteichoic acid

Hsieh, Ching‐Hua; Yang, Johnson Chia‐Shen; Jeng, Jonathan Chris; Chen, Yi‐Chun; Lu, Tsu-Hsiang; Tzeng, Siou-Ling; Wu, Yi-Chan; Wu, Chi‐Chuan; Rau, Cheng-Shyuan

doi:10.1186/1423-0127-20-2

Cited by 21 publications

(15 citation statements)

References 38 publications

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“…In this study, we found that none of the up-regulated miRNAs was expressed in the WBCs. Previously, we had demonstrated a dose- and time-dependent up-regulation of 8 (let-7d, miR-15b, miR-16, miR-25, miR-92a, miR-103, miR-107, and miR-451) and 4 (miR-451, miR-668, miR-1902, and miR-1904) circulating miRNA targets in mice following injection of LPS [11] and LTA [12], respectively. However, in the CLP-based model of sepsis, only miR-16 was up-regulated.…”

Section: Discussionmentioning

confidence: 97%

“…Recently, circulating microRNAs (miRNAs) in the serum were found to show specific expression patterns, and these patterns suggested that serum miRNAs contain fingerprints for various diseases; therefore, circulating miRNAs are considered promising markers for the detection of diseases [10]–[12]. miRNAs are approximately 22-nt-long small regulatory RNA molecules that modulate the activity of specific mRNA targets and play important roles in a wide range of physiologic and pathologic processes [13], [14].…”

Section: Introductionmentioning

confidence: 99%

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Profiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture

Yang

Rau

et al. 2013

PLoS ONE

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The levels of circulating microRNAs (miRNAs) in mice with experimental sepsis induced by cecal ligation and puncture (CLP) were determined using whole blood samples obtained from C57BL/6 mice at 4, 8, and 24 h after CLP; miRNA expression analysis was performed in these samples using an miRNA array. Microarray analysis revealed upregulation of 10 miRNA targets (miR-16, miR-17, miR-20a, miR-20b, miR-26a, miR-26b, miR-106a, miR-106b, miR-195, and miR-451). The expression of these miRNA targets in the whole blood, serum, and white blood cells (WBCs) of CLP mice was quantified using quantitative real-time PCR; these values were compared to those in sham-operated C57BL/6 mice, and the results indicated that these miRNA targets were significantly up-regulated in the whole blood and serum but not in the WBCs. In addition, the levels of these 10 miRNA targets in the serum of Tlr2−/−, Tlr4−/−, and NF-κB−/− mice at 8 h after CLP did not decrease significantly., which indicated that the transcription of these miRNAs was not directly mediated by the TLR2/NF-κB or TLR4/NF-κB pathway, and pathways induced by exposure to the gram-positive or gram-negative bacteria. Immunoprecipitation with the Argonaute 2 ribonucleoprotein complex revealed significantly increased expression of the 10 miRNA targets in the serum of mice after CLP, and the levels of 6 (miR-16, miR-17, miR-20a, miR-20b, miR-26a, and miR-26b) of these 10 miRNA targets increased significantly in exosomes isolated using ExoQuick precipitation solution. In this study, we identified circulating miRNAs that were up-regulated after CLP and determined the increase in the levels of these miRNAs, and our results suggest that circulating Ago2 complexes and exosomes may be responsible for the stability of miRNAs in the serum.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Profiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture

Yang

Rau

et al. 2013

PLoS ONE

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“…DENV infection symptoms are not sufficiently specific to allow clinical differentiation from other acute febrile illnesses, especially in areas where multiple tropical diseases such as malaria, yellow fever, West Nile disease and Saint Louis encephalitis are endemic [6] . There are several dengue vaccine candidates under development, but none is licensed and available [7] . Additionally, there is no specific treatment for dengue, and the most effective protective measures are those that lower the risk of mosquito bites.…”

Section: Introductionmentioning

confidence: 99%

Development, Characterization and Application of Monoclonal Antibodies against Brazilian Dengue Virus Isolates

et al. 2014

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Dengue is the most prevalent human arboviral disease. The morbidity related to dengue infection supports the need for an early, quick and effective diagnostic test. Brazil is a hotspot for dengue, but no serological diagnostic test has been produced using Brazilian dengue virus isolates. This study aims to improve the development of immunodiagnostic methods for dengue virus (DENV) detection through the production and characterization of 22 monoclonal antibodies (mAbs) against Brazilian isolates of DENV-1, -2 and -3. The mAbs include IgG2bκ, IgG2aκ and IgG1κ isotypes, and most were raised against the envelope or the pre-membrane proteins of DENV. When the antibodies were tested against the four DENV serotypes, different reactivity patterns were identified: group-specific, subcomplex specific (DENV-1, -3 and -4 and DENV-2 and -3) and dengue serotype-specific (DENV-2 or -3). Additionally, some mAbs cross-reacted with yellow fever virus (YFV), West Nile virus (WNV) and Saint Louis encephalitis virus (SLEV). None of the mAbs recognized the alphavirus Venezuelan equine encephalitis virus (VEEV). Furthermore, mAbs D3 424/8G, D1 606/A12/B9 and D1 695/12C/2H were used to develop a capture enzyme-linked immunosorbent assay (ELISA) for anti-dengue IgM detection in sera from patients with acute dengue. To our knowledge, these are the first monoclonal antibodies raised against Brazilian DENV isolates, and they may be of special interest in the development of diagnostic assays, as well as for basic research.

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“…Specifically, miR-866-5p was increased in human monocyte-derived macrophages in response to several Mycobacterium avium subsp. hominissuis infection [ 23 ], while a significant increase in miR-668 was observed in circulation following lipoteichoic acid injection, a major component of the wall of gram-positive bacteria [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling

Palmieri

Creanza

Bossa

et al. 2017

IJMS

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Crohn’s disease (CD) is a debilitating inflammatory bowel disease (IBD) that emerges due to the influence of genetic and environmental factors. microRNAs (miRNAs) have been identified in the tissue and sera of IBD patients and may play an important role in the induction of IBD. Our study aimed to identify differentially expressed miRNAs and miRNAs with the ability to alter transcriptome activity by comparing inflamed tissue samples with their non-inflamed counterparts. We studied changes in miRNA–mRNA interactions associated with CD by examining their differential co-expression relative to normal mucosa from the same patients. Correlation changes between the two conditions were incorporated into scores of predefined gene sets to identify biological processes with altered miRNA-mediated control. Our study identified 28 miRNAs differentially expressed (p-values < 0.01), of which 14 are up-regulated. Notably, our differential co-expression analysis highlights microRNAs (i.e., miR-4284, miR-3194 and miR-21) that have known functional interactions with key mechanisms implicated in IBD. Most of these miRNAs cannot be detected by differential expression analysis that do not take into account miRNA–mRNA interactions. The identification of differential miRNA–mRNA co-expression patterns will facilitate the investigation of the miRNA-mediated molecular mechanisms underlying CD pathogenesis and could suggest novel drug targets for validation.

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Circulating microRNA signatures in mice exposed to lipoteichoic acid

Cited by 21 publications

References 38 publications

Profiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture

Profiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture

Development, Characterization and Application of Monoclonal Antibodies against Brazilian Dengue Virus Isolates

Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling

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