Circulating microRNAs as potential biomarkers for coronary plaque rupture

Abstract

Coronary plaque rupture is the most common cause of acute coronary syndrome. However, the timely biomarker-based diagnosis of plaque rupture remains a major unmet clinical challenge. Balloon dilatation and stent implantation during percutaneous coronary intervention (PCI) could cause plaque injury and rupture. Here we aimed to assess the possibility of circulating microRNAs (miRNAs) as biomarkers of acute coronary plaque rupture by virtue of the natural model of PCI-induced plaque rupture. Stable coronary artery disease patients underwent PCI with single stent implantation were recruited and a three-phase approach was conducted in the present study: (i) profiling of plasma miRNAs in a group of patients before (0 h) and after balloon dilatation for 1 h (1 h vs. 0 h), (ii) replication of significant miRNAs in the second group of patients (1 h vs. 0 h), (iii) validation of a multi-miRNAs panel in the third group of patients (0.5 h, 1 h vs. 0 h). Out of 24 miRNAs selected for replication, 6 miRNAs remained significantly associated with plaque rupture. In the validation phase, combinations of miR-483-5p and miR-451a showed the highest area under the receiver-operating-characteristic curve (AUC) (0.982; CI: 0.907-0.999) in patients with plaque rupture for 0.5 h; combinations of miR-483-5p and miR-155-5p showed the highest AUC (0.898; CI: 0.790-0.962) after plaque rupture for 1 h. In conclusion, using a profiling-replication-validation model, we identified 3 miRNAs including miR-155-5p, miR-483-5p and miR-451a, which may be biomarkers for the early identification of plaque rupture.

Effective microRNAs in Ischemia/Reperfusion Before and After Bypass Graft Surgery in Coronary Artery Patients

“…The diagnostic value of miR-21 in the patient group in our study could be considered as moderate. From this point of view, our results are consistent with the literature [24] and the nomination of miR-21 for diagnosis can be con rmed by repeated studies.…”

miR-27b Suppresses Endothelial Cell Proliferation and Migration by Targeting Smad7 in Kawasaki Disease

“…In addition, using DNA-gold nanoparticle probes, miRNA could be better applied in clinical praxis as biomarkers [7]. One research group showed that miR-155-5p, miR-483-5p, and miR-451a may support the early diagnostic value of coronary plaque rupture [8]. In addition to the reports described above, extensive research has indicated that miRNAs play important roles in KD development.…”

MicroRNA‐351‐5p aggravates intestinal ischaemia/reperfusion injury through the targeting of MAPK13 and Sirtuin‐6

“…In order to identify the miRNAs relevant to a pathological condition, such II/R, it is important to find the miRNAs that are differentially expressed in biological samples taken from that condition. An effective method of such analysis is the microarrays assay, a high‐throughput screening method, has been widely used for investigating drug targets and mechanisms of diseases (Li et al ., ; Yuan et al ., ). Thus, the aim of the present work was to find the differentially expressed miRNAs associated with II/R injury by microarrays assay and then to investigate the molecular mechanisms and drug targets for the prevention and treatment of II/R injury.…”