Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients

Cabral, Bianca Catarina Azeredo; Hoffmann, Luísa; Bottaro, Thayane; Costa, Peter; Ramos, Alberto; Coelho, Henrique Sérgio Moraes; Villela-Nogueira, Cristiane Alves; Urmenyi, Turan P.; Faffe, Débora S.; Silva, Rosane

doi:10.1016/j.bbrep.2020.100814

Cited by 20 publications

(11 citation statements)

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“…Similarly, other authors have described an increase in miR34 expression after HCV infection, particularly in serum of patients with chronic hepatitis C ( 38 ). Pairwise, miR34 overexpression has been also correlated with the grade of HCV-dependent cirrhosis, where patients with mild or moderate fibrosis display lower miR34 level expression in the circulating fraction ( 39 ). It has been suggested that the Flaviviridae infection can enhance the expression of miRNAs in target cells to counteract the viral replication ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

HCV Interplay With Mir34a: Implications in Hepatocellular Carcinoma

et al. 2022

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Since its identification, HCV has been considered one of the main causes of hepatitis and liver cancer. Currently, the molecular mechanisms of HCC development induced by HCV infection have not been sufficiently clarified. The recent discovery of novel treatments that inhibit HCV replication gave rise to new questions concerning HCC mechanisms. In particular, the HCV eradication mediated by new direct-acting antiviral (DAAs) drugs does not exclude the possibility of de novo HCC development; this finding opened more questions on the interplay between liver cells and the virus. Different groups have investigated the pathways leading to cancer recurrence in patients treated with DAAs. For this reason, we tried to gain molecular insights into the changes induced by HCV infection in the target liver cells. In particular, we observed an increase in microRNA34a (miR34a) expression following HCV infection of HCC cell line Huh7.5. In addition, Huh7.5 treated with extracellular vesicles (EVs) from the previously HCV-infected Huh7.5 underwent apoptosis. Since miR34 expression was increased in Huh7.5 EVs, we hypothesized a paracrine mechanism of viral infection mediated by miR34a cargo of EVs. The balance between viral infection and cell transformation may raise some questions on the possible use of antiviral drugs in association with antineoplastic treatment.

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Section: Discussionmentioning

confidence: 99%

HCV Interplay With Mir34a: Implications in Hepatocellular Carcinoma

et al. 2022

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“…However, multiple recent studies relied on the serum miR-215 expression levels to differentiate between patients with fibrosis and those with LC and between HCC patients and other hepatic disease patients. It was also observed that miR-215 expression was positively correlated with HCV viral load [54,55], suggesting that miR-215 might act as a potential prognostic biomarker for liver disease.…”

Section: Discussionmentioning

confidence: 94%

Prognostic MicroRNA Panel for HCV-Associated HCC: Integrating Computational Biology and Clinical Validation

Dabbish

Abdelzaher

Abohawya

et al. 2022

Cancers

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Early detection of hepatocellular carcinoma (HCC) will reduce morbidity and mortality rates of this widely spread disease. Dysregulation in microRNA (miRNA) expression is associated with HCC progression. The objective is to identify a panel of differentially expressed miRNAs (DE-miRNAs) to enhance HCC early prediction in hepatitis C virus (HCV) infected patients. Candidate miRNAs were selected using a bioinformatic analysis of microarray and RNA-sequencing datasets, resulting in nine DE-miRNAs (miR-142, miR-150, miR-183, miR-199a, miR-215, miR-217, miR-224, miR-424, and miR-3607). Their expressions were validated in the serum of 44 healthy individuals, 62 non-cirrhotic HCV patients, 67 cirrhotic-HCV, and 72 HCV-associated-HCC patients using real-time PCR (qPCR). There was a significant increase in serum concentrations of the nine-candidate miRNAs in HCC and HCV patients relative to healthy individuals. MiR-424, miR-199a, miR-142, and miR-224 expressions were significantly altered in HCC compared to non-cirrhotic patients. A panel of five miRNAs improved sensitivity and specificity of HCC detection to 100% and 95.12% relative to healthy controls. Distinguishing HCC from HCV-treated patients was achieved by 70.8% sensitivity and 61.9% specificity using the combined panel, compared to alpha-fetoprotein (51.4% sensitivity and 60.67% specificity). These preliminary data show that the novel miRNAs panel (miR-150, miR-199a, miR-224, miR-424, and miR-3607) could serve as a potential non-invasive biomarker for HCC early prediction in chronic HCV patients. Further prospective studies on a larger cohort of patients should be conducted to assess the potential prognostic ability of the miRNAs panel.

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“…Also, it is is negatively correlated with fibrosis in HCV-infected patients [36,37]. miR-885 was found to correlate with different liver pathologies [39][40][41]. Bilirubin is the lipophilic end product of heme breakdown [42].…”

Section: Discussionmentioning

confidence: 99%

Cairo University fibrosis index (CUFI): A score based on microRNA for diagnosis of significant hepatic fibrosis

Abdel-Haleem

Zekri

Moniem

et al. 2022

ijhs

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Introduction: Identification of HCV- induced liver fibrosis is mandatory for tailoring therapy, and management of complications. The current study evaluated the accuracy of circulating miRNAs; in diagnosis of hepatic fibrosis. Patients and methods: Seventy HCV patients were subjected to routine laboratory investigations, HCV-RNA, serum miRNA-122, 221, 192, 224 , 375, and 885 by PCR, liver biopsy and calculation of the following scores: aspartate aminotransferase (AST)/alanine aminotransferase (ALT) ratio (AAR), aspartate to platelet ratio index (APRI), FIB-4 score, Hui index, Fibrosis Index (FI), Fibro-Q , Fibro-Alfa Biotechnology Research Center (BRC) score and Gotebörg University Cirrhosis Index (GUCI). Results: Patients with significant and advanced fibrosis have significantly lower miR-122 (P < 0.0001 and P= 0.007, respectively). miR-122, bilirubin and miR-855 were found to be independent predictors of significant fibrosis in univariate analysis. A novel score; Cairo University Fibrosis Index (CUFI) based on microRNA 122, bilirubin and microRNA 855 were formulated for predicting significant liver fibrosis. The AUC of this score, for predicting significant and advanced hepatic fibrosis was 0.83 and 0.80 respectively. This AUC was higher than those of other fibrosis scores. Conclusion: Cairo University Fibrosis Index is better than the existing scores in assessing fibrosis in chronic HCV patients.

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Circulating microRNAs associated with liver fibrosis in chronic hepatitis C patients

Cited by 20 publications

References 50 publications

HCV Interplay With Mir34a: Implications in Hepatocellular Carcinoma

HCV Interplay With Mir34a: Implications in Hepatocellular Carcinoma

Prognostic MicroRNA Panel for HCV-Associated HCC: Integrating Computational Biology and Clinical Validation

Cairo University fibrosis index (CUFI): A score based on microRNA for diagnosis of significant hepatic fibrosis

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